The effects of different washing techniques, including the combined use of heated
water and microbubbles water, on fresh-cut lettuce leaves (Lactuca
sative var, capitata) were examined. The outer leaves were removed
from the lettuce and cut into squares with dimensions of approximately 1–2 cm.
The lettuce leaf samples were then washed with either tap water (Tap),
microbubbles water (MB, Contains 70% or more of microbubbles with a
diameter of 10μm or less), heated water (HWT, 40°C, 45°C or
50°C), or heated microbubbles water (HWT+MB, 40°C,
45°C or 50°C) for 1 min. After washing, the microbiological
properties (i.e., total viable bacteria, coliform and fungi) and quality of the
samples were measured. The total viable bacteria level of the HWT-50°C
sample was <1 log CFU/g, representing the most effective washing
technique, although the freshness decreased rapidly during storage. In addition,
HWT+MB-40°C treatment was less effective in reducing the
microbiological levels, although the decay rate was maintained at 0% for
the initial 2 d of storage, and showed the lowest value of 11% after 6 d.
Furthermore, the decay rate for the sample treated with MB was
<30% after 10 d, and a similar result was obtained for the
HWT+MB-40°C sample. It was apparent that a combined heat and
microbubbles treatment, and in particular at a temperature of 40°C, was
effective for maintaining the freshness and quality of fresh-cut lettuce during
storage.