The current study was performed to develop biodegradable films with matrix composed from gum Arabic (GA) and different concentrations from Alpinia officinarum phenolic extract (AOE). The color indices, physical properties, surface shape, crystallinity, mechanical properties and thermal stability of produced films were investigated. The incorporation of AOE extract (0.25 g/100 mL) inside GA films increased dark yellow color, reduced moisture, swelling, solubility and water vapor permeability (WVP) of films up to 10.29%, 21.05%, 14.15% and 4.20 × 10−10 g H2O/m s p.a., respectively. Furthermore, the scanning electron micrographs showed uniform structure with rough surface and turmoil spots in the structure by increasing AOE concentration inside films. The FT-IR analysis showed typically bands that proved physical interaction between AOE extract and GA polymer. The fabricated films showed satisfied thermal stability manners with declining trend by incorporation of AOE inside GA films. Furthermore, the developed films showed significant effects on reducing the changes of browning index from 17.20 to 24.54 and firmness from 33.98 to 22.65 N of Agaricus bisporus caps during storage days compared with commercial packaging materials.