The article presents the results of training motivation study in the system of labor motivation among the fishing industry employees on the example of fishing trawler employees from LLC "Russian Fish Industry Company" (Vladivostok). The fishing industry was selected due to the high importance of this area for the regional economy development, the large number of employees involved, and because of rapid changes in production technologies that require timely personnel training. The company development strategy is to modernize production and increase labor productivity, which requires the provision of the industry with the personnel motivated to acquire new knowledge and master modern technologies. The study was carried out to identify the features of motivation to train employees as a mechanism for the professional development implementation. The methodological basis of the study was the psychological and sociological approach to the study of the work motive structure and the construction of motivational profiles based on a comparative analysis of the results in the context of age groups and various job categories. The main research method used was the survey of employees, as well as the statistical methods of data processing. Thus, significant differences were established between employees during evaluation the generally accepted motives of work, and their significance was determined; the level of motivation for training and the characteristics of the motivational sphere of the ship personnel were revealed; according to the degree of importance of motives and their severity, the interviewed employees formed motivational profiles, obtained data on the subjective assessment of their qualification level; they studied the factors contributing to the desire for professional development increase. In order to optimize the processes of employee motivation management, it is proposed to use individual approaches to motivate training, and improve training methods. The conclusion is made about the positive influence of changes on the training effectiveness and the activities of employees in general in the motivation system.