Quality by design based development and validation of HPLC method for simultaneous estimation of paclitaxel and vinorelbine tartrate in dual drug loaded liposomes

Bonde, Smita; Bonde, Chandrakant; Prabhakar, Bala

doi:10.1016/j.microc.2019.103982

Cited by 41 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, there are a large number of methods to quantify PAC, including HPLC, HPLC-MS, and UPLC-MS ( Bonde et al, 2019 ; Christner et al, 2019 ; Attia et al, 2020 ), which take a long time and cost a lot. On the contrary, there are very few reports on the quantization of paclitaxel by electrochemical methods ( Gowda and Nandibewoor, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior and Direct Quantitative Determination of Paclitaxel

et al. 2022

View full text Add to dashboard Cite

The electrochemical behavior and direct quantitative determination of paclitaxel, a poorly soluble drug made into microemulsion, were researched by cyclic voltammetry in acetate buffer solutions (pH = 4.0) at a glassy carbon electrode. The results show that the oxidation process is irreversible and controlled by diffusion. Moreover, the effects of anodic peak current (Ipa), anodic peak potential, scan rate, pH, and the electrochemical redox mechanism have been studied. The anodic peak current varied linearly with paclitaxel concentration in the range of 5 × 10−5 mol/L to 5 × 10−4 mol/L, and the detection limit was 9.15 × 10−8 mol/L. The results of RSD (0.90%) and recovery (99.22%–101.69%) were obtained. Additionally, it has been proved that one electron and one proton are involved in the electrochemical redox process. The present research has been successfully used to determine paclitaxel in pure and real samples, which further supported the electrochemical behavior investigation of paclitaxel and direct determination of micro-emulsion.

show abstract

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior and Direct Quantitative Determination of Paclitaxel

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The mobile phase was a mixture of acetonitrile and water (30 mM ammonium acetate adjusted to pH 3.2 using ortho-phosphoric acid, v/v = 55:45), and the flow rate was set at 1 mL·min −1 , at ambient temperature. 31 Each measurement was carried out in triplicate. EE and DL were calculated according to the following equations.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Antitumor Efficacy Mediated by Liposomal Co-Delivery of Polymeric Micelles of Vinorelbine and Cisplatin in Non-Small Cell Lung Cancer

Wang¹,

Gou²,

Wang³

et al. 2021

IJN

View full text Add to dashboard Cite

Purpose Non-small cell lung cancer (NSCLC) is an aggressive tumor with high mortality and poor prognosis. In this study, we designed a liposome encapsulating polymeric micelles (PMs) loaded with vinorelbine (NVB) and cis-diamminedichloroplatinum (II) (cisplatin or CDDP) for the treatment of NSCLC. Materials and Methods Sodium poly(α- l -glutamic acid)-graft-methoxy-polyethylene glycol (PLG-G-PEG 5K ) was used to prepare NVB-loaded NVB-PMs and CDDP-loaded CDDP-PMs that were co-encapsulated into liposomes by a reverse evaporation method, yielding NVB and CDDP co-delivery liposomes (CoNP-lips) composed of egg phosphatidyl lipid-80/cholesterol/DPPG/DSPE-mPEG 2000 at a molar ratio of 52:32:14:2. The CoNP-lips were characterized in terms of particle size, zeta potential, drug content, encapsulation efficiency, and structural properties. Drug release by the CoNP-lips as well as their stability and cytotoxicity was evaluated in vitro, and their antitumor efficacy was assessed in a mouse xenograft model of Lewis lung carcinoma cell-derived tumors. Results CoNP-lips had a spherical shape with uniform size distribution; the average particle size was 162.97±9.06 nm, and the average zeta potential was −13.02±0.22 mV. In vitro cytotoxicity analysis and the combination index demonstrated that the CoNP-lips achieved a synergistic cytotoxic effect at an NVB:CDDP weight ratio of 2:1 in an NSCLC cell line. There was sustained release of both drugs from CoNP-lips. The pharmacokinetic analysis showed that CoNP-lips had a higher plasma half-life than NP solution, with 6.52- and 8.03-fold larger areas under the receiver operating characteristic curves of NVB and CDDP. CoNP-lips showed antitumor efficacy in tumor-bearing C57BL/6 mice and drug accumulation in tumors via the enhanced permeability and retention effect. Conclusion CoNP-lips are a promising formulation for targeted therapy in NSCLC.

show abstract

“…The multifactor optimization by QbD improves the suitability of the method by decreasing the need for revalidation. 28…”

Section: Analytical Target Profilementioning

confidence: 99%

Response Surface Optimization for Simultanous Estimation of Sofosbuvir and Velpatasvir in Human Plasma Sample

2021

View full text Add to dashboard Cite

An efficient column friendly, buffer free, highly sensitive, cost effective RP-HPLC method was developed by considering the criticality of different method parameters on analytical attributes like tailing factor, resolution and retention time in preliminary risk analysis and screening designs. The Pareto analysis of screening design highlighted the need for optimization of resolution and its influencers (capacity factor and theoretical plates) for both the drugs to imbibe quality in the method. The suggested method of optimization design was developed using ZODIAC C18 ODS (250 mm × 4.6 mm, 5 μm) column in isocratic mode using mobile phase acetonitrile : methanol : water in the ratio of 60:10:30 at a flow rate of 0.8 mL/min and UV detection wavelength of 262 nm. The retention times of drugs were found to be 3.488 minutes for sofosbuvir, 5.387 minutes for velpatasvir. The linear regression analysis data for the calibration plots showed good linear relationship with r2=0.997 for sofosbuvir, r2=0.988 for velpatasvir, in the working concentration range of 1000-5000 ng/mL, 250-1250 ng/mL respectively. The AQbD devised method was applied for quantification of drugs in plasma and validated as suggested in ICH M10 guidelines.

show abstract

Quality by design based development and validation of HPLC method for simultaneous estimation of paclitaxel and vinorelbine tartrate in dual drug loaded liposomes

Cited by 41 publications

References 24 publications

Electrochemical Behavior and Direct Quantitative Determination of Paclitaxel

Electrochemical Behavior and Direct Quantitative Determination of Paclitaxel

Synergistic Antitumor Efficacy Mediated by Liposomal Co-Delivery of Polymeric Micelles of Vinorelbine and Cisplatin in Non-Small Cell Lung Cancer

Response Surface Optimization for Simultanous Estimation of Sofosbuvir and Velpatasvir in Human Plasma Sample

Contact Info

Product

Resources

About