Quality by Design in optimizing the extraction of (poly)phenolic compounds from Vaccinium myrtillus berries

Marzullo, Luca; Ochkur, Oleksandr; Orlandini, Serena; Renai, Lapo; Gotti, Roberto; Коshovyi, Oleh; Furlanetto, Sandra; Bubba, Massimo Del

doi:10.1016/j.chroma.2022.463329

Cited by 19 publications

(17 citation statements)

References 43 publications

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“…Both American cranberries and European cranberries accumulate a high level of valuable phenolics. European cranberries contain higher amounts of caffeic acid, quercetin, and flavonols than American cranberries [ 28 , 29 , 30 ]. The differences in the composition of polyphenolics in cranberries may be affected by various conditions such as weather, maturity stage, cultivation, harvesting, region, etc.…”

Section: Discussionmentioning

confidence: 99%

American Cranberry (Oxycoccus macrocarpus (Ait.) Pursh) Leaves Extract and Its Amino-Acids Preparation: The Phytochemical and Pharmacological Study

Коshovyi

Власова

Jakštas

et al. 2023

Plants

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The liver is an organ with several important biofunctions, for which there are very few effective and safe preparations that promote the functioning, protection, and regeneration of cells. Sufficiently safe preparations with hepatoprotective activity can be found in plants. The aim of our study was to investigate the chemical composition of an extract made from American cranberry (Oxycoccus macrocarpus (Ait.) Pursh) leaves and its amino-acids preparations as well as their possible hepatoprotective activity. Using the UPLC-MS/MS method, we identified 19 phenolic compounds (8 flavonoids (flavones and flavonols), 4 anthocyanins, 3 hydroxycinnamic acids, and 2 catechins). The prophylactic and therapeutic administration of the American cranberry-leaves extracts led to a decrease in the lipid-peroxidation process during a study of tetrachloromethane acute toxic damage in the liver of rats. As a result of animal studies, the most effective hepatoprotective activity was found in the extract preparations with valine and arginine.

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Section: Discussionmentioning

confidence: 99%

American Cranberry (Oxycoccus macrocarpus (Ait.) Pursh) Leaves Extract and Its Amino-Acids Preparation: The Phytochemical and Pharmacological Study

Коshovyi

Власова

Jakštas

et al. 2023

Plants

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“…There are a limited number of studies focused on the design of phytocompounds extraction, mainly on non-edible residues, such as sea buckthorn leaves [11][12][13], winery by-products [14,15], apples [16] and potato peels [17][18][19]. The polyphenols extraction from bilberry pomace was studied through central composite design and response surface methodology (RSM) [20], while bilberry fruit extraction using the Box-Behnken design [7] or RSM [21] was also reported. A study of bilberry leaf extract after LED irradiation was also performed [22].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Polyphenols Extraction from Wild Bilberry Leaves—Antimicrobial Properties and Stability Studies

et al. 2023

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Polyphenolic extracts from natural sources have received great interest due to their beneficial properties for human health. A method to reduce their variability is to use the design of experiments which allows a limited number of experiments to be performed while exploring the experimental space. Firstly, a 23-full factorial model was used to investigate the polyphenols extraction from wild bilberry leaves. Spectrophotometric data (the content of polyphenols, flavonoids, chlorophyll and radical scavenger activity) and extraction yield were used as responses, and six statistical models were determined depending on the two numerical factors (temperature and alcohol % of ethanol–water mixture) being significant (p < 0.05) in all cases. Numerical optimisation performed by Design Expert 13 software correlates well with the chemical profile determined by high-performance liquid chromatography and the amount of the polyphenol. Afterwards, under the optimised conditions, an extract was prepared in three extraction steps for which composition, chemical stability and antimicrobial properties were evaluated. The antimicrobial potential of the extract was compared with that of the standard compounds (rutin and chlorogenic acid), and the results supported a synergistic effect of the extract components.

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“…Using these results ( Table 3 ), the optimization of the extraction process was carried out using each stage’s mass yield coefficient (m i BAS /V i extractant ) of the different compounds [ 32 , 33 ]. The diagram of these compounds’ dependence on the extraction rate is shown in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of Matricaria discoidea DC. with Analgesic and Soporific Activities

Sepp,

Koshovyi,

Jakštas

et al. 2024

Biomolecules

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Pineapple weed (Matricaria discoidea DC.) is a widespread plant in Europe and North America. In ethnomedicine, it is well-known for its anti-inflammatory and spasmolytic activities. The aim of this research was to develop novel methods of M. discoidea processing to obtain essential oil and dry extracts and to investigate their phytochemical compositions. Moreover, the molecular docking of the main substances and the in vivo studies on their soporific and analgesic activities were conducted. The essential oil and two dry extracts from M. discoidea were prepared. A total of 16 phenolic compounds (seven flavonoids, seven hydroxycinnamic acids, and two phenolic acids) in the dry extracts were identified by means of UPLC-MS/MS. In the essential oil, nine main terpenoids were identified by gas chromatography (GC). It was shown that phenolic extraction from the herb was successful when using 70% ethanol in a triple extraction method and at a ratio of 1:14–1:16. The in vivo studies with rodents demonstrated the analgesic activity of the M. discoidea extracts and improvements in the sleep of animals. The dry extracts of M. discoidea did not show any toxicity. The molecular docking analysis showed a high probability of COX-1,2 inhibition and NMDA receptor antagonism by the extracts.

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Quality by Design in optimizing the extraction of (poly)phenolic compounds from Vaccinium myrtillus berries

Cited by 19 publications

References 43 publications

American Cranberry (Oxycoccus macrocarpus (Ait.) Pursh) Leaves Extract and Its Amino-Acids Preparation: The Phytochemical and Pharmacological Study

American Cranberry (Oxycoccus macrocarpus (Ait.) Pursh) Leaves Extract and Its Amino-Acids Preparation: The Phytochemical and Pharmacological Study

Optimisation of Polyphenols Extraction from Wild Bilberry Leaves—Antimicrobial Properties and Stability Studies

Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of Matricaria discoidea DC. with Analgesic and Soporific Activities

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