Quality Control in Flow Cytometry

Barnett, David; Reilly, John T.

doi:10.1007/978-1-59745-451-3_4

Cited by 4 publications

(3 citation statements)

References 59 publications

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“…The inconclusive agreement regarding the use of a daily quality control check of the flow cytometer using standardized fluorescent calibration beads may be related to the fact that this statement referred not only to the need to run a quality control, but also that this should be done daily and using calibration beads. Certain instruments or flow cytometry applications (e.g., in hematology) require stabilized blood and not calibrated beads as quality control 45 . This may have contributed to the uncertain rating of this statement, and should not be taken as an uncertainty around the need for calibration.…”

Section: Discussionmentioning

confidence: 99%

“…Certain instruments or flow cytometry applications (e.g., in hematology) require stabilized blood and not calibrated beads as quality control. 45 This may have contributed to the uncertain rating of this statement, and should not be taken as an uncertainty around the need for calibration. Guidance on this topic as well as panel design may be drawn from recent reviews.…”

Section: Discussionmentioning

confidence: 99%

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Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function: Communication from the ISTH SSC Subcommittee on Platelet Physiology

Frelinger

Rivera

Connor

et al. 2021

Journal of Thrombosis and Haemostasis

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Flow cytometry is increasingly used in the study of platelets in inherited and acquired disorders of platelet number and function. However, wide variation exists in specific reagents, methods, and equipment used, making interpretation and comparison of results difficult. The goal of the present study was to provide expert consensus guidance on the use of flow cytometry for the evaluation of platelet disorders. A modified RAND/UCLA survey method was used to obtain a consensus among 11 experts from 10 countries across four continents, on the appropriateness of statements relating to clinical utility, pre‐analytical variables, instrument and reagent standardization, methods, reporting, and quality control for platelet flow cytometry. Feedback from the initial survey revealed that uncertainty was sometimes due to lack of expertise with a particular test condition rather than unavailable or ambiguous data. To address this, the RAND method was modified to allow experts to self‐identify statements for which they could not provide expert input. There was uniform agreement among experts in the areas of instrument and reagent standardization, methods, reporting, and quality control and this agreement is used to suggest best practices in these areas. However, 25.9% and 50% of statements related to pre‐analytical variables and clinical utility, respectively, were rated as uncertain. Thus, while citrate is the preferred anticoagulant for many flow cytometric platelet tests, expert opinions differed on the acceptability of other anticoagulants, particularly heparin. Lack of expert consensus on the clinical utility of many flow cytometric platelet tests indicates the need for rigorous multicenter clinical outcome studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function: Communication from the ISTH SSC Subcommittee on Platelet Physiology

Frelinger

Rivera

Connor

et al. 2021

Journal of Thrombosis and Haemostasis

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show abstract

“…The second step verifies the stability of the signal acquired over time. A common practice to verify the quality of signal acquisition is to use Levy-Jennings-type graphs, where fluorescence is plotted against time (Barnett and Reilly, 2007). A stable signal acquisition should produce intensity values whose distribution is consistent throughout the course of the entire experiment.…”

Section: Signal Acquisition Checkmentioning

confidence: 99%

flowAI: automatic and interactive anomaly discerning tools for flow cytometry data

et al. 2016

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Recommendations for the development and validation of flow cytometry‐based receptor occupancy assays

Green

Stewart

Högerkorp

et al. 2016

Cytometry Part B Clinical

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Receptor occupancy measurements demonstrate the binding of a biotherapeutic agent to its extracellular target and represent an integral component of the pharmacodynamic (PD) portfolio utilized to advance the development and commercialization of a therapeutic agent. Coupled with traditional pharmacokinetic (PK) assessments derived from serum drug concentration, receptor occupancy data can be used to model PK/PD relationships and validate dose selection decisions throughout the drug development lifecycle. Receptor occupancy assays can be even more challenging to develop than other flow cytometric methods (e.g. surface immunophenotyping). In addition to typical considerations regarding stability of the cell type of interest, stability of the target-bound therapeutic agent and stability of the target receptor must be taken into account. Reagent selection is also challenging as reagents need to be evaluated for the potential to compete with the therapeutic agent and bind with comparable affinity. This article provides technical guidance for the development and validation of cytometry-based receptor occupancy assays. V C 2016 International Clinical Cytometry Society

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Quality Control in Flow Cytometry

Cited by 4 publications

References 59 publications

Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function: Communication from the ISTH SSC Subcommittee on Platelet Physiology

Consensus recommendations on flow cytometry for the assessment of inherited and acquired disorders of platelet number and function: Communication from the ISTH SSC Subcommittee on Platelet Physiology

flowAI: automatic and interactive anomaly discerning tools for flow cytometry data

Recommendations for the development and validation of flow cytometry‐based receptor occupancy assays

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