Quality control method of sterols in fermented Cordyceps sinensis based on combined fingerprint and quantitative analysis of multicomponents by single marker

Abstract

In this study, we established a new pattern for differentiating and comprehensively evaluating the quality of fermented Cordyceps sinensis based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), principal component analysis (PCA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). These methods indicated that fermented Cordyceps sinensis samples could be categorized into one class by PCA and HCA. The fingerprints of fermented Cordyceps sinensis were established, and four HPLC peaks were identified as ergosterol, daucosterol, stigmasterol, and β-sitosterol in Jinshuibao capsules and tablets (two products of fermented Cordyceps sinensis). Ergosterol was chosen as the internal reference substance, and the relative correction factors (RCFs) between ergosterol and the other three sterols were calculated using the QAMS method. Moreover, the accuracy of the QAMS method was confirmed by comparing the relative error between the results of the method used with those of an external standard method (ESM). No significant difference between the two methods was observed. The total sterols content in Jinshuibao products were calculated by the QAMS method, and the total sterols content of the two products were similar. This study showed that the method established herein was efficient and successful in the identification fermented Cordyceps sinensis and may further act to facilitate systematic quality control of fermented Cordyceps sinensis products.

Unsaturated fatty-acid based HPLC fingerprints in combination with quantitative analysis of multi-components by single-marker for the classification of Rana chensinensis ovum

“…In this study, ALA was designated as an internal reference, and the RCFs of UFAs to be measured were calculated according to eqn (1) at the injection volumes of 2, 6, 10, 14, 18 μL. 17,33 where F s/i indicates the RCF of the internal reference ALA relative to the UFA to be measured, f s and f i represent absolute correction factors of the internal reference ALA and UFAs to be measured, respectively, C i and A i are the concentration (mg mL −1 ) and peak area (mAU) of UFAs to be measured, C s and A s are the concentration (mg mL −1 ) and peak area (mAU) of the internal reference ALA.…”

Chemical components in cultivated Cordyceps sinensis and their effects on fibrosis

“…The previous studies showed that nucleosides suppressed inflammation in the progress of chronic obstructive pulmonary disease ( Sun et al, 2018b ), inhibited the epithelial mesenchymal transition and protected against renal fibrosis in diabetic nephropathy ( Dong, Sun, Wei, Li, & Zhao, 2019b ). Sterols are also very important bio-functional components in fungi, and were separated or identified from C. sinensis by many researchers ( Chen et al, 2021 , Siwulski et al, 2019 , Sun et al, 2017 , Zhao et al, 2020 ). Ergosterol, a principal sterol, has been used as a useful chemical marker for evaluating the quality of C. sinensis ( Yuan, Wang, Liu, Kuang, & Zhao, 2007 ).…”

Evaluation of Cordyceps sinensis Quality in 15 Production Areas Using Metabolomics and the Membership Function Method

“…In the past 20 years, C. sinensis, as a good functional food and drug development raw material, has been the subject of published studies, with a lot of good comments, but its edible value and medicinal value depend on its origin [17]. Different sources of C. sinensis have different sensory qualities, metabolite compositions, and flavors [18].…”