Quality Control of Psoralea corylifolia L. Based on High-Speed Countercurrent Chromatographic Fingerprinting

Abstract

Traditional Chinese medicine (TCM)has played an important role in promoting the health of Chinese people. The TCM Psoralea corylifolia L. has been used in the treatment of various kinds of diseases including enuresis, vitiligo, and calvities. However, therapeutic effects of P. corylifolia L. have often influenced by the quality of plants. So, it is very important to control the quality of P. corylifolia L. In this study, analytical high-speed countercurrent chromatography (HSCCC) was successfully used to fingerprint P. corylifolia L. Samples of P. corylifolia L. were extracted by ultrasonic extraction. n-hexane-ethyl acetate–methanol–water at a ratio of 5:5.5:6.5:5 (v/v) was selected as a two-phase solvent system and the condition of HSCCC were optimized in order to good separation. And the method of HSCCC was verified (reproducibility, precision, and stability). HSCCC chromatograms exhibited six common peaks, which were selected as indicator compounds for the quality control of P. corylifolia L. Within 20 types of medicinal materials, chemical components are similar, but the levels of components are quite different in HSCCC fingerprint. The present results demonstrate that the HSCCC method provides a reliable basis for the quality control of P. corylifolia L. and can also be applied to confirm the authenticity of plant materials.

Psoralea corylifolia L.: a comprehensive review of its botany, traditional uses, phytochemistry, pharmacology, toxicology, quality control and pharmacokinetics

“…A study used HSCCC fingerprint to control the quality of PCL, it was found that the chemical components of 20 batches of Chinese herbal medicines were similar, but their contents were quite different. At the same time, it was confirmed that the HSCCC approach had good reproducibility, precision, and stability, providing a solid foundation for the quality control of PCL and having the potential to be utilized to identify plant medicinal materials [ 177 ]. By combining the HPLC fingerprint and multi-component analysis, the quality of PCL was assessed.…”

Combined Ultrahigh Pressure Extraction and High-Speed Counter-Current Chromatography for Separation and Purification of Three Glycoside Compounds from Dendrobium officinale Protocorm

“…Generally, the K D value for the target component is usually expected to be between 0.5 and 2.0. If the K D value is under 0.5, the solute will be eluted near the solvent front, which may result in peak resolution loss and poor separation performance; if the K D value is much greater than 2.0, the solute will elute in an excessively broad peak and may lead to a longer elution time, reduced column efficiency, and wasted solvent [ 36 , 37 , 38 ]. Meanwhile, the stationary phase retention can reflect the exaction rate during HSCCC separation.…”

Integrative Review on the Chemical Components, Pharmacology and Toxicology of Psoralea Corylifolia L. (Bu Gu Zhi)

“…3c). [3][4][5] Other components in PCL mainly include trace elements such as potassium, manganese, calcium and selenium, lipids such as monoglyceride, diglyceride, triglyceride and free fatty acid, glycosides such as daucosterol, methylglycoside and PCL polysaccharide, as well as fatty acids in volatile oils and non-volatile terpenoid oils such as palmitic acid, oleic acid, linoleic acid and stearic acid.…”