2021
DOI: 10.3791/62785
|View full text |Cite
|
Sign up to set email alerts
|

Quality-Controlled Sputum Analysis by Flow Cytometry

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

0
5
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
3

Relationship

2
1

Authors

Journals

citations
Cited by 3 publications
(5 citation statements)
references
References 0 publications
0
5
0
Order By: Relevance
“…2 ). The FCM component of the test consists of two assay tubes, one labelled with blood cell markers and one with epithelial cell markers [ 25 ]. Cells in both tubes were also labeled with anti-CD45 antibodies which selectively bind leukocytes, a viability dye to eliminate dead cells, including squamous epithelial cells (SECs) [ 27 ], and TCPP to identify cancer or cancer-associated cells [ 28 ].…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…2 ). The FCM component of the test consists of two assay tubes, one labelled with blood cell markers and one with epithelial cell markers [ 25 ]. Cells in both tubes were also labeled with anti-CD45 antibodies which selectively bind leukocytes, a viability dye to eliminate dead cells, including squamous epithelial cells (SECs) [ 27 ], and TCPP to identify cancer or cancer-associated cells [ 28 ].…”
Section: Resultsmentioning
confidence: 99%
“…Sputum was dissociated and labeled using recently published protocols [ 25 , 26 ]. Briefly, sputum samples were incubated with a mixture of 0.1% dithiothreitol and 0.5% N -acetyl- l -cysteine for 15 min at room temperature and neutralized with Hank’s Balanced Salt Solution (HBSS).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Sputum was liquefied using pre-warmed 0.1% dithiothreitol (DTT) at a 1:4 ratio with sputum weight (w/v) and pre-warmed 0.5% N-acetyl-L-cysteine (NAC) at a ratio of 1:1 (w/v), as described previously [ 43 ]. The resulting cell suspension was filtered through 100 μm nylon cell strainers (Falcon, Corning Inc.) to eliminate larger debris while minimizing cell loss ( S1 Fig ).…”
Section: Methodsmentioning
confidence: 99%
“…Cell labeling was performed as described previously [ 43 ]. Briefly, the majority of cells were split into two tubes: one including markers to interrogate the leukocyte (CD45 + ) cell compartment and one for the epithelial (CD45 - ) cell compartment.…”
Section: Methodsmentioning
confidence: 99%