Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

Abstract

Sutchi Catfish is one of the important fish commodities in Indonesia. Unfortunately, its seasonal spawning pattern causes limited supply. Cryopreservation is a solution to solve limited supply since it can store the spermatozoa in low temperature so that physiological, biological and morphological functions still remain. Improving the quality of cryopreservation is important to increase the success of Sutchi Catfish aquaculture. Adding honey in cryopreservation process is expected to increase the quality of spermatozoa since it contains with sugars as a source of spermatozoa's energy. This study tried to compare the effectivity of honey in cryopreservation process with no addition. The treatments used in this study were T1 (0% honey), T2 (0.2% of honey), T3 (0.4% of honey), T4 (0.6% of honey) and T5 (0.8% of honey). 30 days after stored, the spermatozoa were checked their motility, viability, abnormality, fertility and hatching rate. This study showed that honey addition could increase the motility significantly (P<0.01) to 23.14% better than control. The viability increased significantly (P<0.01) to 23.17% better than control. The abnormality test did not show significant difference between honey addition and control although the abnormality value in control was the highest (10.75%). The fertilization rate increased significantly (P<0.01) to 28.85% better than control. The hatching rate increased significantly (P<0.01) to 29.78% better than control. The success of all test indicated that the addition of honey in cryopreservation process of spermatozoa could be performed on Sutchi Catfish to increase its production even though the limited spawning pattern.

Creation of a Biobank of the Sperm of the Honey Bee Drones of Different Subspecies of Apis mellifera L.

“…For example, Malik et al (2017) [51], experimenting with the replacement of glycerol with honey at a concentration of 8%, revealed a significant increase in abnormalities in sperm morphology (8.35 ± 0.16%), while the viability (82.19 ± 1.41%) and motility (76.63 ± 3.21%) did not differ significantly from those of fresh sperm. Shikh Maidina et al (2018) and Fanni et al (2018) [53,57], on the contrary, did not find major changes in sperm morphology but noted frequent changes in the head and tail of the flagellum. Other authors [54] reported a lower percentage of dead and abnormal sperm when using honey.…”

“…Honey has proven antimicrobial (antibacterial, antimycotic, antimycobacterial) properties, the interest in which has recently been growing [49]. The addition of honey to an extender for cryopreservation significantly improves sperm motility after thawing and the integrity of membranes and acrosomes and also reduces the number of abnormalities in sperm morphology in horses [50], bulls [51], buffalo [52], goats [53], sheep [54], rats and mice [55,56], fish [57,58], and humans [59].…”