Quality evaluation of Flos Lonicerae through a simultaneous determination of seven saponins by HPLC with ELSD

Chai, Xingyun; Li, Songlin; Li, Ping

doi:10.1016/j.chroma.2005.02.031

Cited by 106 publications

(63 citation statements)

References 3 publications

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“…Chlorogenic acid, an ester formed between caffeic acid and quinic acid (Fig.3), is one of the most naturally existing phenolic compounds found in numerous plant species and also a major component of Flos Lonicerae that has long been regarded as one of the most important chemical standards for quality evaluation of Flos Lonicerae (Chai et al, 2005). It was widely reported that chlorogenic acid and related compounds are well known to be antioxidants (Kono et al, 1997;Ohnishi et al, 1994).…”

Section: Contribution Of Chlorogenic Acid To Antioxidant Activity Of mentioning

confidence: 99%

“…In many previous reports, chlorogenic acid, one of the major components in Flos Lonicerae, has been widely adopted to control the quality of Flos Lonicerae owing to its high content and antibiotic property. Several kinds of the other components, such as flavonoids, iridoid glucosides and saponins have been also discovered and characterized using advanced analytical techniques due to their effective activities (Chai et al, 2005;Chang and But, 1986;Chen et al, 2007;Li et al, 2003;Song et al, 2006;Zhang et al, 2006). Therefore great progress has been made in the studies and clinical applications of Flos Lonicerae in the last couple of years (Chang and But, 1986;MPHPRC, 2000;Shi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts

Lan

2007

J. Zhejiang Univ. - Sci. B

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Abstract:Flos Lonicerae is a medically useful traditional Chinese medicine herb. However, little is known about the antioxidant properties of Flos Lonicerae extracts. Here the antioxidant capacity of water, methanolic and ethanolic extracts prepared from Flos Lonicerae to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and reduce Fe 3+ to Fe 2+ is examined. Chlorogenic acid, a major component of Flos Lonicerae, is identified and further purified from 70% ethanolic extract with high performance liquid chromatography (HPLC) and its antioxidant capacity is characterized. The total phenolic compounds and chlorogenic acid contents in Flos Lonicerae are determined. The present results demonstrate that the Flos Lonicerae extracts exhibit antioxidant activity and chlorogenic acid is a major contributor to this activity.

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Section: Contribution Of Chlorogenic Acid To Antioxidant Activity Of mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts

Lan

2007

J. Zhejiang Univ. - Sci. B

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“…15,16) Bilirubin is another structural type of bioactive component in Calculus Bovis, whereas, by ELSD detection, it has weak response and very poor sensitivity, which might be due to its instability under light and heating conditions. [17][18][19] Therefore, bilirubin is often analyzed by UV detection, and to evaluate the quality of Calculus Bovis, bile acids and bilirubin have to be determined by different detection techniques, and thus duplicated analyses are often required. To the best of our known, no approach has been developed to simultaneously determine these two kinds of components.…”

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confidence: 99%

Comparative Study on Major Bioactive Components in Natural, Artificial and in-Vitro Cultured Calculus Bovis

Yan

Liu

et al. 2007

Chem. Pharm. Bull.

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Major bioactive components in various Calculus Bovis, including natural, artificial and in-vitro cultured Calculus Bovis, were comparatively studied. An approach of high-performance liquid chromatography coupled with ultraviolet and evaporative light scattering detections (HPLC/UV/ELSD) was established to simultaneously determinate six bioactive components thereof, including five bile acids (cholic acid, deoxycholic acid, ursodeoxycholic, chenodeoxycholic acid, hyodeoxycholic acid) and bilirubin. ELSD and UV detector were applied to detect bile acids and bilirubin respectively. The assay was performed on a C 18 column with water-acetonitrile gradient elution and the investigated constituents were authenticated by comparing retention times and mass spectra with those of reference compounds. The proposed method was applied to analyze twenty-one Calculus Bovis extraction samples, and produced data with acceptable linearity, precision, repeatability and accuracy. The result indicated the variations among Calculus Bovis samples under different developmental conditions. Artificial and invitro cultured Calculus Bovis, especially in-vitro cultured ones, which contain total bioactive constituents no less than natural products and have the best batch-to-batch uniformity, suffice to be used as substitutes of natural Calculus Bovis.

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“…9 For saponins analysis, the literature provides several types of methods, among including spectrophotometric methods, 10 TLC, 11,12 HPLC (with different detection methods: UV, MS, ELSD), [13][14][15][16][17] gas chromatography 18 and capillary electrophoresis 19 .…”

Section: Introductionmentioning

confidence: 99%

Comparative Quantification of Escin From Different Products

Corciovă¹,

Ivănescu²

2017

ECB

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The paper aimed to quantify the saponins expressed in escin, the major bioactive constituent of Aesculus hippocastanum, from five different pharmaceutical formulations: two types of tablets, and one type of gel, tincture, and glycerol-hydroalcoholic extract. The products are part of over the counter drugs and dietary supplements categories. Two spectrophotometric methods were used to quantify the escin, after separation from the other active ingredients and excipients. The first method, calibration curve method, is based on the reaction between the oxidized triterpenoid saponins and vanillin and records the absorbance at 560 nm. The limit of detection, limit of quantification, and the RSD values were calculated. The second method is based on the molar absorptivity of escin. Both methods have proved to be suitable for the determination of escin in different products.

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Quality evaluation of Flos Lonicerae through a simultaneous determination of seven saponins by HPLC with ELSD

Cited by 106 publications

References 3 publications

Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts

Effect of chlorogenic acid on antioxidant activity of Flos Lonicerae extracts

Comparative Study on Major Bioactive Components in Natural, Artificial and in-Vitro Cultured Calculus Bovis

Comparative Quantification of Escin From Different Products

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