Quality evaluation of Lonicerae Japonicae Flos from different origins based on high‐performance liquid chromatography (HPLC) fingerprinting and multicomponent quantitative analysis combined with chemical pattern recognition

Zhang, Jie; Yu, Xiao; Yang, Ran; Zheng, Bingqing; Zhang, Yongqing; Zhang, Fang

doi:10.1002/pca.3319

Cited by 6 publications

(1 citation statement)

References 33 publications

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“…The chromatographic conditions were optimized with reference to the work of Liu et al [38] and Zhang et al [39]. Chromatographic analysis was performed on a highperformance liquid chromatography system (Prominence LC-20A, Shimadzu Corporation, Kyoto, Japan) equipped with a UV detector and a quadruple pump.…”

Section: Hplc Analysismentioning

confidence: 99%

Enhanced Antioxidant Extraction from Lonicerae japonicae Flos Based on a Novel Optimization Strategy with Tailored Deep Eutectic Solvents

Deng,

Sun,

Yang

et al. 2024

Separations

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Lonicerae japonicae Flos (LJF) is a natural plant containing abundant antioxidant ingredients. In order to extract more antioxidants from LJF, in this study, a novel strategy was proposed for optimizing the extraction factor level by response surface methodology with a tailored deep eutectic solvent (DES) as the extraction solvent and antioxidant ability as the evaluation index. After optimizing the composition of DES and the extraction condition, the extracts obtained by our proposed method yielded better antioxidant ability (229.1–249.1 μmol TE/g DW) and higher antioxidant contents (34.2–36.5 mg GAE/g DW for total phenolics and 119.6–123.0 mg RE/g DW for total flavonoids) from LJF in 5 min without organic solvent consumption that were significantly superior to the Chinese Pharmacopoeia extraction method. The K-T solvation parameter and a scanning electron microscope were adopted to explore the extraction mechanism, and the results showed that the polarity and damage effect on plant cells of DES were crucial for the extraction of antioxidants. In addition, after combining the HPLC fingerprint and partial least squares model, chlorogenic acid, rutin, and 3,5-O-Dicaffeoylquinic acid were screened as the antioxidant Q-markers of LJF. This work demonstrates that an optimization strategy based on antioxidant ability and tailored DES has the potential to extract more antioxidants from natural plants.

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Section: Hplc Analysismentioning

confidence: 99%

Enhanced Antioxidant Extraction from Lonicerae japonicae Flos Based on a Novel Optimization Strategy with Tailored Deep Eutectic Solvents

Deng,

Sun,

Yang

et al. 2024

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Quality markers of Polygala fallax Hemsl decoction based on qualitative and quantitative analysis combined with network pharmacology and chemometric analysis

Li,

Cheng,

Zhang

et al. 2024

Phytochemical Analysis

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IntroductionPolygala fallax Hemsl (PFH) is a widely used herbal medicine in Guangxi, China. At present, research on PFH mainly focuses on extraction technology and cultivation, lacking quality control standards for systematic evaluation.ObjectivesThe study aimed to assess the quality of PFH from different sources and to predict markers that would help assess quality.MethodsFingerprinting of 15 batches of PFH samples was performed by ultra‐high performance liquid chromatography (UPLC) and similarity was assessed using hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal partial least squares discrimination (OPLS‐DA). Differential components were screened by mathematical analysis, and a “component‐target‐pathway” network map was constructed in combination with network pharmacology, quality markers (Q‐markers) of PFH were predicted, and quantitative analysis was performed.ResultsFifteen batches were fingerprinted for PFH, with 11 common peaks, and peak 5 was identified as 4‐hydroxybenzoic acid, which was generally consistent with the results of HCA, PCA, and OPLS‐DA. Network pharmacology screened 18 potential compounds, 45 core targets, and 20 key pathways, integrating fingerprinting, pattern recognition, and network pharmacology methods. One of the potential Q‐markers that can identify the principle of testability, efficacy, and specificity is 4‐hydroxybenzoic acid, whose content ranges from 0.0188 to 1.4517 mg/g.ConclusionThe potential Q‐markers of PFH were predicted by integrating fingerprinting, pattern recognition, and network pharmacological analysis, which provided a scientific basis for the overall control and evaluation of the quality of PFH and a theoretical reference for the study of the quality standard of multi‐base traditional Chinese medicine.

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Detection and quantification of lactoferrin: Innovations, applications, and challenges

Abedi-Firoozjah,

Behnam,

Balouch Zehi

et al. 2025

Food Chemistry

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Quality evaluation of Lonicerae Japonicae Flos from different origins based on high‐performance liquid chromatography (HPLC) fingerprinting and multicomponent quantitative analysis combined with chemical pattern recognition

Cited by 6 publications

References 33 publications

Enhanced Antioxidant Extraction from Lonicerae japonicae Flos Based on a Novel Optimization Strategy with Tailored Deep Eutectic Solvents

Enhanced Antioxidant Extraction from Lonicerae japonicae Flos Based on a Novel Optimization Strategy with Tailored Deep Eutectic Solvents

Quality markers of Polygala fallax Hemsl decoction based on qualitative and quantitative analysis combined with network pharmacology and chemometric analysis

Detection and quantification of lactoferrin: Innovations, applications, and challenges

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