Quality Evaluation of Ophiopogonis Radix from Two Different Producing Areas

Tan, Mengxi; Chen, Jiali; Wang, Yuming; Zou, Lisi; Chen, Shuyu; Shi, Jingjing; Mei, Yuqi; Wei, Lifang; Liu, Xunhong

doi:10.3390/molecules24183220

Cited by 26 publications

(22 citation statements)

References 16 publications

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“…In order to evaluate the content difference of 22 constituents in xylem and phloem, t-test analysis was used and the values of p less than 0.05 were considered remarkably different [20,21]. As illustrated in Figure 6, more than half of the bioactive constituents investigated in this study showed significant difference between xylem and phloem.…”

Section: T-test Of Samplesmentioning

confidence: 94%

“…The standards of procyanidin B2 (5), daidzin (7), dihydroquercetin (8), genistin (9), liquiritigenin (12), calycosin (14), naringenin (15), genistein (16), medicarpin (20), prunetin (21) and biochanin A (22) were purchased from Nanjing Liangwei biotechnology Co., Ltd. (Nanjing, China). Epicatechin (6), rutin (10), kaempferol (17) and formononetin (19) were purchased from the Chinese National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China).…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…The samples were analyzed by orthogonal partial least squares discriminant analysis (OPLS-DA) to find out the differential constituents which were associated with the sample classifications. T-tests were performed to show the difference of each compound between the two sections of SC [17][18][19][20][21]. The proposed investigation could bring to light the distribution patterns of metabolites in xylem and phloem of SC and provide the basis for exploring the accumulation rule of metabolites in SC.…”

Section: Introductionmentioning

confidence: 99%

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A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

et al. 2019

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Spatholobi Caulis (SC), the vine stem of Spatholobus suberectus Dunn, is a widely used traditional Chinese medicine (TCM) for the treatment of blood stasis syndrome and related diseases. Xylem and phloem are the main structures of SC and the color of xylem in SC is red brown or brown while the phloem with resin secretions is reddish brown to dark brown. They are alternately arranged in a plurality of concentric or eccentric rings. In order to investigate the distribution patterns of metabolites in xylem and phloem of SC, an analytical method based on UFLC–QTRAP–MS/MS was established for simultaneous determination of 22 constituents including four flavanols, nine isoflavones, two flavonols, two dihydroflavones, one flavanonol, one chalcone, one pterocarpan, one anthocyanidin and one phenolic acid in the samples (xylem and phloem) from Laos. Furthermore, according to the contents of 22 constituents, heat map, principal components analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS–DA) and t–test were used to evaluate the samples and discover the differences between xylem and phloem of SC. The results indicated that the measured ingredients in xylem and phloem were significantly different. To be specific, the contents of flavonoids in xylem were higher than that in phloem, while the content of protocatechuic acid showed a contrary tendency. This study will not only reveal the distribution patterns of metabolites in xylem and phloem of SC but also facilitate further study on their quality formation.

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Section: T-test Of Samplesmentioning

confidence: 94%

Section: Chemicals and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

et al. 2019

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“…It also enables the simultaneous determination of multiple compounds, which greatly shortens analysis time [27]. In summary, this technique possesses remarkable superiority in selectivity, sensitivity, and analysis capability, so it has been widely used for the separation and analysis of metabolites in medicinal materials [28][29][30]. Herein, the UFLC-QTRAP-MS/MS technology was adopted for the simultaneous determination of twenty-one analytes in FF.…”

Section: Selection Of Determination Methodsmentioning

confidence: 99%

Distribution Patterns for Bioactive Constituents in Pericarp, Stalk and Seed of Forsythiae Fructus

et al. 2020

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Forsythiae Fructus (FF) is a widely used folk medicine in China, Japan, and Korea. The distribution of bioactive constituents throughout the fruit segments has rarely been addressed, although mounting evidence suggests that plant secondary metabolites are synthesized and distributed regularly. The phytochemical profiles of three segments of FF (pericarp, stalk and seed) were firstly revealed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitative analysis of twenty-one bioactive constituents, including three phenylethanoid glycosides, five lignans, eight flavonoids, and five phenolic acids to explore the spatial distribution of bioactive constituents. Furthermore, the hierarchical clustering analysis (HCA) and one-way analysis of variance (one-way ANOVA) were conducted to visualize and verify the distribution regularity of twenty-one analytes among three segments. The results showed that phytochemical profiles of the three segments were similar, i.e., phenylethanoid glycosides covering the most part were the predominant compounds, followed by lignans, flavonoids and phenolic acids. Nevertheless, the abundance of twenty-one bioactive constituents among three segments was different. Specifically, phenylethanoid glycosides were highly expressed in the seed; lignans were primarily enriched in the stalk; flavonoids were largely concentrated in the pericarp, while the contents of phenolic acids showed no much difference among various segments. The research improves our understanding of distribution patterns for bioactive constituents in FF, and also complements some scientific data for further exploring the quality formation mechanism of FF.

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“…Tan M.X. et al[29] applied ultra-fast performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) to measure 36 components of Ophiopogonis Radix and found the bioactive constituents in Ophiopogonis Radix from different regions were signi cantly different; Shu Z. et al[30] employed reversed-phase high-performance liquid chromatography coupled with hierarchical clustering analysis to evaluate the quality of Vitex negundo seeds, and observed signi cant differences in the content of lignans of Vitex negundo seeds from different origins; Cao Y. et al[31] in order to evaluate the quality consistency of Chansu, established HPLC ngerprints of Chansu and discovered that the ngerprint pro le of Chansu from different geographical origins were different.…”

mentioning

confidence: 99%

A Combined Quality Evaluation Method That Integrated Chemical Constituents, Appearance Traits and Origins of Raw Rehmanniae Radix Pieces

Yuan²,

Qin³

et al. 2020

Preprint

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Background The quality control of traditional Chinese medicine (TCM) is a challenge for internationalization of TCM. Modern quality evaluation methods ignore origins and appearance traits, and traditional quality evaluation methods lack quantitative analysis. Therefore, an integrated quality evaluation method is urgent in need. Raw Rehmanniae Radix (RRR) was a widely used TCM. Its quality has caught much attention, and the existing quality evaluation methods have certain limitations. The aim of this study was to establish a comprehensive and practical method for the quality evaluation and control of RRR pieces based on its chemical components, appearance traits and origins.Methods 33 batches of RRR pieces were collected from 6 provinces, high performance liquid chromatography (HPLC) was applied to determine 5 constituents including catalpol, rehmannioside A, rehmannioside D, leonuride and verbascoside in RRR pieces. The appearance traits were qualitatively observed. Furthermore, correlation analysis, principal components analysis (PCA), cluster analysis and t-test were performed to discriminate and evaluate different quality of RRR pieces. Results 33 batches of RRR pieces could be divided into three categories, the samples of Henan province were in a group, the samples from Shandong province and Shanxi province were classified in a group, and the samples from other provinces were divided in a group. Furthermore, the constituents and appearance traits of RRR pieces were significantly different in diverse origins. Conclusions The combined method of chemical components, appearance traits and origins could classify and distinguish different quality of RRR pieces, which could provide a basic reference to quality control of TCM.

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Quality Evaluation of Ophiopogonis Radix from Two Different Producing Areas

Cited by 26 publications

References 16 publications

A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

A Method to Study the Distribution Patterns for Metabolites in Xylem and Phloem of Spatholobi Caulis

Distribution Patterns for Bioactive Constituents in Pericarp, Stalk and Seed of Forsythiae Fructus

A Combined Quality Evaluation Method That Integrated Chemical Constituents, Appearance Traits and Origins of Raw Rehmanniae Radix Pieces

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