Quality Evaluation of Oviductus Ranae Based on PUFAs Using HPLC Fingerprint Techniques Combined with Chemometric Methods

Abstract

Oviductus Ranae is a nutritional product for both medicine and food. Its quality is uneven due to multiple factors. An efficient method was established to evaluate the quality of Oviductus Ranae using fingerprint techniques and chemometric methods based on the analysis of polyunsaturated fatty acids (PUFAs) in petroleum ether extract by high performance liquid chromatography (HPLC). During this process, 27 batches of Oviductus Ranae were analyzed by HPLC and two types of chromatographic fingerprints were established. The fingerprint that was obtained by matching six known peaks was used for the quantification of six PUFAs. Another fingerprint was obtained by matching sixteen peaks with a peak area ratio greater than 0.5% and it was used to classify the different qualities of Oviductus Ranae by further combining three different chemometric models. The 27 batches of Oviductus Ranae were divided into four categories, which was consistent with the analysis results of six PUFAs contents. The two high-quality samples with significantly higher contents were classified into one category, and samples with medium contents were divided into two categories, including eight and thirteen samples, respectively. The four inferior samples with lower contents were classified into one category. The results indicated that the newly developed method has potential application prospects for the quality evaluation of Oviductus Ranae.

High‐performance liquid chromatography–based fingerprint analysis with chemical pattern recognition for evaluation of Mahonia bealei (Fort.) Carr

“…The values of the three parameters range between 0 and 1. A reliable model could be obtained if these values are the closer to 1 [26].…”

Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

“…Among them, UFAs, as important bioactive substances, have excellent biological activities in regulating immunity and preventing a series of cardiovascular and cerebrovascular diseases such as hyperlipidemia, hypertension and thrombosis [ 10 , 11 , 12 , 13 , 14 ]. The content of UFA is considered to be closely related to the quality of Oviductus Ranae [ 15 ]. However, due to the influence of breeding environment, production technology and preservation conditions [ 2 ], the content of UFA in Oviductus Ranae in different regions varies, resulting in the uneven quality of Oviductus Ranae in the market [ 15 ].…”

“…The content of UFA is considered to be closely related to the quality of Oviductus Ranae [ 15 ]. However, due to the influence of breeding environment, production technology and preservation conditions [ 2 ], the content of UFA in Oviductus Ranae in different regions varies, resulting in the uneven quality of Oviductus Ranae in the market [ 15 ]. Therefore, it is necessary to develop an economical and convenient method to monitor the content of UFA in Oviductus Ranae to ensure its quality.…”

Rana chensinensis Ovum Oil Based on CO2 Supercritical Fluid Extraction: Response Surface Methodology Optimization and Unsaturated Fatty Acid Ingredient Analysis

“…Due to the relatively small amount of research on the by-products, RCO is usually treated as waste, causing serious wastage of Rana chensinensis resources. In our previous research, we found that Rana chensinensis ovum oil (RCOO) was rich in a variety of unsaturated fatty acids (UFAs), and its UFA content is several times higher than that of Oviductus Ranae [ 7 ]. Currently, UFAs are attracting much attention due to their extensive physiological activities [ 8 , 9 ].…”