Quality Evaluation of Traditional Chinese Drug Toad Venom from Different Origins through a Simultaneous Determination of Bufogenins and Indole Alkaloids by HPLC

Abstract

A simple and efficient HPLC method has been developed to evaluate the quality of traditional Chinese medicine toad venoms. The major bioactive ingredients, including 10 bufogenins and 4 indole alkaloids in the drug, were separated and quantified on a phenyl-hexyl column with a UV detector. A total of 27 toad venom samples from two species, Bufo bufo gargarizans CANTOR and Bufo melanostictus SCHNEIDER, from the different drug production regions of China, were analyzed. The chromatograms showed significant differences with respect to the samples from different origins. These toad venom samples can be distinctly classified into 4 groups by cluster analysis using the contents of the 14 main constituents, including toad venom samples from B. bufo gargarizans from north China, median China and south China, and samples from B. melanostictus from south China. Toad venom samples from B. bufo gargarizans from median China were considered to be of the highest quality.

Discrimination of cinnamon bark and cinnamon twig samples sourced from various countries using HPLC-based fingerprint analysis

“…Moreover, HPLC methods, in combination with pattern 0308-8146/$ -see front matter Ó 2011 Elsevier Ltd. All rights reserved. doi:10.1016/j.foodchem.2011.01.011 analysis, have been used to successfully classify and discriminate different herbs (Feng et al, 2001;Zhang et al, 2005;Wan, Yang, Li, Wang, & Cui, 2006). Although many previous studies have reported on the quality of cinnamon using TLC, GC-MS, HPLC, or pattern analysis (Gong, Liang, Xu, & Chau, 2001;Gong, Liang, & Fung, 2004;Ross, 1976;Wen, Huang, & Liu, 1992;Zhu, Liu, Luo, & Bu, 1996;), to the best of our knowledge, we are the first to comparatively study CB and CT using discriminant analysis.…”

Efficacy and safety of gemcitabine-oxaliplatin combined with huachansu in patients with advanced gallbladder carcinoma

“…In addition, more and more people have paid their close attention to Chinese medicines for the prevention and treatment of cancer. Huachansu (cinobufagin) is just a widely used antitumor agent of traditional Chinese medicine in China [10,11] . Because the clinical data have demonstrated the effectiveness of gemcitabine on pancreatic cancer, and gallbladder shares a common embryological origin with the exocrine pancreas, we used gemcitabine-oxaliplatin (GEMOX) combined with huachansu injection (cinobufagin) in treatment of advanced GBC.…”

Comprehensive chemical analysis of Venenum Bufonis by using liquid chromatography/electrospray ionization tandem mass spectrometry

“…The quality control of VB is critical for its safety and effective usage in clinic. Several quality control methods have been carried out by using TLC, HPLC and GC analysis [25][26][27][28][29]. However, most of these investigations mainly focus on the analysis of the free-type hydrophobic bufadienolides.…”

“…Through the METLIN accurate mass matching database analysis, yield 97 preliminary matches. Among them, 30 high quality matches were obtained through a further comparison of both their accurate mass and retention times with those in our personal database (AMRT database) established according to the literatures [1][2][3][27][28][29]. Their MRM chromatograms in four solvent extracts of VB were shown in Fig.…”