Quality Evaluation of Tricholoma matsutake Based on the Nucleic Acid Compounds by UPLC-TOF/MS and UPLC-QqQ/MS

Abstract

So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, 2′-deoxyuridine, adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, and uridine 5′-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake.

A Practical Method for Determination of Nine Nucleosides in Tricholoma matsutake by UPLC/MS and Quantitative Analysis of Multicomponents Using Single Marker Method

“…After extraction, experiments were performed to optimize the UPLC/MS method. A total of 17 nucleoside compounds have previously been successfully separated using a porous graphitic carbon column by our team [ 7 , 17 ]. Since the use of porous graphitic carbon columns is not common, conventional C18 analytical columns were selected in this work for the purpose of developing a QAMS UPLC/MS method with good universality.…”

“…We used boiling water to extract nucleosides from T. matsutake, as previously described [7,21]. A room temperature ultrasonic extraction method is more commonly reported for the extraction of nucleosides in the literature [16][17][18][19], perhaps due to the convenience of the method.…”

“…Nucleic acid constituents that can regulate various physiological processes in vivo through the purine/pyrimidine receptors are considered suitable markers for the quality evaluation of T. matsutake [ 7 , 8 ]. Sichuan Food and Drug Administration commissioned researchers from several units, including our group, to jointly draft local standards for the safety of T. matsutake and its products, which were implemented on July 20, 2018.…”

“…On this basis, our team conducted an in-depth study on the active ingredients in T. matsutake and proposed for the first time that nucleic acid compounds be used as the quality control markers for commercial T. matsutake products. Subsequently, a validated UPLC/MS method based on the external standard method was developed to determine the content of each nucleic acid compound in 80 samples from different regions of Sichuan Province [ 7 ]. However, the external standard method, as a classical quantitative test method, requires the purchase of all reference substances and preparation of each corresponding solution as well as other operations, which may be considered wasteful of time and money.…”

Identification of new trace related impurities in adenosine products using ultra‐high‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

“…Several chromatographic procedures such as high‐performance liquid chromatography (HPLC) with ultraviolet detection (UV), thin‐layer chromatography (TLC), capillary electrochromatography (CEC), LC/mass spectrometry (MS), or LC/MS/MS have been adopted for the analysis of adenosine and its related impurities. However, these techniques are not suitable for structural determination of unknown compounds at trace levels.…”