Quality management in cardiovascular tissue engineering

Jockenhoevel, S; Kanert, A; E, Strehlau; Cornelissen, Christian; Glasmacher, Birgit; Chalabi, Khaled; Sachweh, JS; Vázquez, John Alexander Campuzano

doi:10.1055/s-2005-922361

Cited by 2 publications

(2 citation statements)

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“…[26] However, it is not mechanically strong enough to withstand the physiological pressure in the vascular networks of our body. [27] It can be combined with suitable matrices to improve mechanical strength and support vascularization. The silk scaffolds, besides enhancing the attachment and proliferation of cells, can guide the formation of capillary-like structures in the fibrin gel as the cell distribution in the gel alone is not very uniform.…”

Section: Introductionmentioning

confidence: 99%

Co‐Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk–Fibrin Scaffolds Supports Vascularization

Samal

Weinandy²,

Weinandy

et al. 2015

Macromolecular Bioscience

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A successful strategy to enhance the in vivo survival of engineered tissues would be to prevascularize them. In this study, fabricated silk fibroin scaffolds from mulberry and non-mulberry silkworms are investigated and compared for supporting the co-culture of human umbilical vein endothelial cells and human foreskin fibroblasts. Scaffolds are cytocompatible and when combined with fibrin gel support capillary-like structure formation. Density and interconnectivity of the formed structures are found to be better in mulberry scaffolds. ELISA shows that levels of vascular endothelial growth factor (VEGF) released in co-cultures with fibrin gel are significantly higher than in co-cultures without fibrin gel. RT PCR shows an increase in VEGFR2 expression in mulberry scaffolds indicating these scaffolds combined with fibrin provide a suitable microenvironment for the development of capillary-like structures.

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Section: Introductionmentioning

confidence: 99%

Co‐Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk–Fibrin Scaffolds Supports Vascularization

Samal

Weinandy²,

Weinandy

et al. 2015

Macromolecular Bioscience

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“…There are also efforts based on the use of the biopolymer fibrin, as used herein. The Jockenhoevel group molds valves from fibrin gel containing entrapped cells 9 in a completely biological approach similar to ours, although with key differences. For example, longitudinal shortening that we exploit to achieve circumferential alignment in the root is blocked in their approach.…”

Section: Introductionmentioning

confidence: 99%

Implantation of a Tissue-engineered Heart Valve from Human Fibroblasts Exhibiting Short Term Function in the Sheep Pulmonary Artery

Syedain

Lahti

Johnson

et al. 2011

Cardiovasc Eng Tech

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Abstract-We have previously demonstrated the feasibility of fabricating a fibrin-based tissue-engineered heart valve (TEHV) using neonatal human dermal fibroblasts (nhDF), including leaflets with structural and mechanical anisotropy similar to native leaflets. The aim here was to evaluate the performance of this TEHV in a pilot study using the sheep model. Bi-leaflet TEHV were conditioned in a cyclic stretching bioreactor, then implanted within a polymeric sleeve interpositionally into the pulmonary artery of four sheep, with the pulmonary valve either left intact or rendered incompetent. Heparin and immunosuppression were administered for the duration. Echocardiography was performed at implantation and at 4 and 8 weeks. Explants were examined histologically, biochemically, and mechanically. In all sheep, echocardiography at implantation showed coapting leaflets, with minimal valve regurgitation and no turbulence. Orifice area and pressure gradients at systole approached the native pulmonary valve values. Echocardiography at 4 weeks revealed both leaflets functional with moderate regurgitation and turbulence in three sheep; in one sheep, only one leaflet was evident. Explanted leaflets had thickness and tensile properties comparable to the implanted leaflets. There was extensive endothelialization of the root lumenal surface. In the two sheep continued to 8 weeks, only one shortened leaflet remained in both cases. Immunocytochemistry indicated this was due to sustained tissue contraction caused by the nhDF and not by the invading host cells, which included a subpopulation consistent with bone marrow-derived cells. Short-term success was thus achieved in terms of excellent valve function at implantation and some valve function for at least 4 weeks; however, an apparent progressive tissue contraction needs to be resolved for long-term success.

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Quality management in cardiovascular tissue engineering

Cited by 2 publications

References 0 publications

Co‐Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk–Fibrin Scaffolds Supports Vascularization

Co‐Culture of Human Endothelial Cells and Foreskin Fibroblasts on 3D Silk–Fibrin Scaffolds Supports Vascularization

Implantation of a Tissue-engineered Heart Valve from Human Fibroblasts Exhibiting Short Term Function in the Sheep Pulmonary Artery

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