Quality Markers of Dendrobium officinale by “Oligosaccharide-Spectrum-Effect” Relationships

Abstract

Dendrobium officinale Kimura et Migo has been used as a traditional Chinese medicine (TCM) and a functional food for thousands of years. Carbohydrate is one of the most important effective substances and indicative components in D. officinale. However, since the qualitative and quantitative analysis of polysaccharides in D. officinale remains a challenge and limitation, herein, an oligosaccharide-quality marker approach was newly developed for quality assessment of D. officinale by spectrum–effect relationships between high performance liquid chromatographic (HPLC) fingerprints and anti-inflammatory effects. The HPLC fingerprints of 48 batches of oligosaccharides from D. officinale (DOOS) were developed and analyzed with similarity analysis (SA) and hierarchical cluster analysis (HCA), and eight common peaks were identified. In vitro screening experiment indicated that DOOS potentially inhibited nitric oxide (NO) production and effectively reduced the release of inflammatory cytokines, such as TNF-α, IL-6, and IL-1β in RAW 264.7 cells, thereby reducing the inflammatory response of cells. Finally, the HPLC fingerprint of different batches of DOOS was combined with in vitro anti-inflammatory activity to assess the spectrum–effect relationships of DOOS by gray correlation analysis (GCA), in addition, the purified oligosaccharide components were identified and validated for NO inhibitory activity. Our results showed four DOOS (maltotetraose, maltopentaose, maltohexaose, and mannohexaose) were relevant to anti-inflammatory effects and could be as quality markers for the quality control of D. officinale. It suggests that the “oligosaccharide-spectrum-effect” relationships approach is a simple and reliable method for the quality control of herb medicines or nutritious foods.

Oligosaccharides fromAsparagus cochinchinensisfor ameliorating LPS-induced acute lung injury in mice

“…29 It is suggested that the natural oligosaccharides present in the plant have a lower molecular weight and greater solubility, foreshadowing that oligosaccharides may exhibit similar or even greater activity. 30 Therefore, oligosaccharides, the main components in the water decoction of Asparagi radix, may be worth investigating and are of great significance for the development of Asparagus cochinchinensis .…”

“…32 TCM oligosaccharides play an important role in nutrition, healthcare, and disease prevention and have broad application prospects in the field of medicine. Numerous in vitro and in vivo experiments exhibited that oligosaccharides possess various activities, including anti-inflammatory, 28,30 antidiabetic, 33,34 antidepressant, 35,36 antioxidant, 37 antineoplastic, 38 anti-ageing, immunopotentiation, enhanced memory and neuroprotective, 39 which have been revealed in recent years. Furthermore, its anti-inflammatory property is one of the most important biological effects of Asparagi radix.…”

Qualitative and quantitative method for quality control of Itea ilicifolia based on antioxidant Q‐markers

“…Although Q‐marker research is still in its infancy, several research strategies have been developed to discover and identify quality markers, such as systems pharmacology, pharmacokinetic analysis, chimidomics integrating serum pharmacochemistry and metabolomics technique, chemical‐activity‐based systematic screening, and so on (Lu, Jin, et al, 2022; Ren et al, 2020). Among them, the spectrum–effect relationship‐based strategy, which correlates the biological and chemical information and reflects the key components related to efficacy and safety, has been a general practice for Q‐marker screening (Li et al, 2019; Liu et al, 2022; Lu, Li, et al, 2022).…”