Quality of chilled and cold-stored (5 °C) canine spermatozoa submitted to different rapid cooling rates

Rodenas, Carmen; Parrilla, Inmaculada; Roca, Jordi; Rodríguez‐Martínez, H.; Lucas, Xiomara

doi:10.1016/j.theriogenology.2014.05.022

Cited by 6 publications

(7 citation statements)

References 30 publications

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“…Our finding agrees with the study of Rijsselaere et al, which noted a significant decline in TM and PM over the 72 h of cooling period independent of the centrifugation groups [ 10 ]. Other canine studies also reported negative effects of 24–96 h of cold storage on sperm motility variables [ 38 , 39 ]. This finding is similar to studies in other species, mainly stallions, where PM declines from initial to 48–72 h of cooled storage [ 20 , 40 ].…”

Section: Discussionmentioning

confidence: 95%

Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing

Sugai

Werre

Cecere

et al. 2023

Animals

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Our objective was to determine a clinically relevant range of centrifugation parameters for processing canine semen. We hypothesized that higher gravitational (g) force and longer time of centrifugation would result in improved spermatozoa recovery rate (RR) but poorer semen quality. Cooled storage under standard shipping conditions was used as a stressor to evaluate long-term treatment effects. Individual ejaculates collected from 14 healthy dogs were split into six treatment groups (400 g, 720 g, and 900 g for 5 or 10 min). Sperm RR (%) was calculated post-centrifugation, and plasma membrane integrity (%, Nucleocounter® SP-100™), total and progressive motility (%, subjective and computer-assisted sperm analysis), and morphology (%, eosin-nigrosin staining) were assessed on initial raw semen (T0), post-centrifugation (T1), and 24 h (T2) and 48 h (T3) after cooling. Sperm losses were minimal, and RRs were similar across treatment groups (median >98%, p ≥ 0.062). Spermatozoa membrane integrity was not different between centrifugation groups at any time point (p ≥ 0.38) but declined significantly during cooling (T1 vs. T2/T3, p ≤ 0.001). Similarly, total and progressive motility did not differ across treatments but declined in all groups from T1 to T3 (p ≤ 0.02). In conclusion, our study showed that centrifugation within a range of 400 g–900 g for 5–10 min is appropriate for processing canine semen.

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Section: Discussionmentioning

confidence: 95%

Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing

Sugai

Werre

Cecere

et al. 2023

Animals

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“…They can also prevent premature sperm capacitation and acrosome reaction (Witte et al 2009). It is noteworthy that egg yolk is a conventional but effective extender for canine sperm preservation and cryopreservation (Silva et al 2002;Ponglowhapan et al 2004;Verstegen et al 2005;Shahiduzzaman & Linde-Forsberg 2007;Witte et al 2009;Batista et al 2012;Goericke-Pesch et al 2012;Treulen et al 2012;Rodenas et al 2014;Hori et al 2017). However, the results also indicated that although soybean lecithin extenders were lower than egg yolk Table 6.…”

Section: Discussionmentioning

confidence: 99%

“…; Rodenas et al . ). Recently, soybean lecithin used as an alternative to egg yolk in extender to avoid hygiene problems from bacterial contamination has obtained equal or superior results (Beccaglia et al .…”

Section: Introductionmentioning

confidence: 97%

“…To improve the quality of chilled sperm during storage time, spermatozoa are diluted with an appropriate extender to provide energy, maintain pH and osmolality and protect the plasma membrane integrity, acrosome membrane integrity, mitochondrial membrane potential and DNA fragmentation against damage. In previous studies, the Tris-citric-fructose or glucose extender with 20% egg yolk was considered one of the most common extenders for chilled canine sperm that best maintains the quality of sperm during cooling storage (Rota et al 1995;Ponglowhapan et al 2004;Verstegen et al 2005;Shahiduzzaman & Linde-Forsberg 2007;Batista et al 2012;Goericke-Pesch et al 2012;Rodenas et al 2014). Recently, soybean lecithin used as an alternative to egg yolk in extender to avoid hygiene problems from bacterial contamination has obtained equal or superior results (Beccaglia et al 2009a,b;Kmenta et al 2011;Kasimanickam et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Nguyen

Ponchunchoovong

Kupittayanant

et al. 2019

Veterinary Medicine & Sci

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The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer‐assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate‐conjugated Pisum sativum agglutinin (FITC‐PSA) and 5,5′,6,6′‐tetrachloro‐1,1′,3,3′‐tetraethylbenzimidazolyl‐carbocyanine iodide (JC‐1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris‐citric‐fructose extender could maintain the motility better than other extenders, egg yolk in Tris‐citric‐fructose‐mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris‐citric‐fructose‐mineral salts extender was lower than that of soybean lecithin in Tris‐citric‐fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris‐citric‐fructose or Tris‐citric‐fructose‐mineral salts extenders, and egg yolk in Tris‐citric‐fructose‐mineral salts extender is superior to other extenders in chilling canine sperm.

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“…In contrast to frozen semen, chilled semen requires fewer special techniques and inexpensive equipment for processing and transportation (Hori et al, 2017). But to preserve the quality of semen, chilled sperm has a limited shelf life, necessitating the use of extenders (Rodenas et al, 2014). According to Bencharif et al (2013), semen preservation during liquid storage can also be aided by factors like refrigeration speed and storage temperature.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Freezing and Chilling Effects on Survival and Oxidative Characteristics of Funaab Alpha (Normal Feather) Chicken Sperm

OJO,

OGUNJIMI,

DARAMOLA

2024

FUDMAJAAT

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The impact of freezing and chilling on the semen of FUNAAB Alpha (Normal feather) chickens was examined in this study. Pooled semen collected from ten FUNAAB Alpha (Normal feather) chicken were diluted with Tris –based extender. Diluted semen was divided into two fraction, the first fraction was subjected to chilling at 4oC and the second fraction was subjected to freezing at 1oC for 24hrs. Thereafter semen were evaluated for viability, functional integrity and oxidative stress (Malondialdehyde concentrations MDA and seminal leukocytes). The result showed a consistent variation in the viability and functional integrity. However, Sperm motility and livability preserved at 4oC was significantly higher (p<0.05) than those preserved at 1oC. Abnormal sperm (head, mid piece and tail) recorded for those preserved at 4oC was significantly lower (p<0.05) than those preserved at 1oC. The findings indicate that sperm viability, functional integrities and oxidative stress parameters of FUNAAB alpha (Normal feather) were better preserved by chilling compared to freezing method.

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Quality of chilled and cold-stored (5 °C) canine spermatozoa submitted to different rapid cooling rates

Cited by 6 publications

References 30 publications

Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing

Defining an Optimal Range of Centrifugation Parameters for Canine Semen Processing

Effects of egg yolk and soybean lecithin on sperm quality determined by computer‐assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm

Comparative Study of Freezing and Chilling Effects on Survival and Oxidative Characteristics of Funaab Alpha (Normal Feather) Chicken Sperm

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