Quality of leucoreduced red blood cell concentrates: 5 years of follow‐up in France

Chabanel, A.; Carrat, Fabrice; Bégué, S.; Masse, M; Perrault, M; Andreu, G.

doi:10.1111/j.1423-0410.2007.00981.x

Cited by 17 publications

(8 citation statements)

References 6 publications

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“…It is possible that they indicate normal quality fluctuations between different donor groups due to seasonal variability. Indeed, samples collected in the warmest month (Group 1, collected in September) had the poorest storage quality, consistent with results that have been reported elsewhere and have been associated with the residual white blood cell count after leukoreduction (24). All RBC units in our study had a leukocyte count <10E6/unit.…”

Section: Discussionsupporting

confidence: 89%

“…Indeed, samples collected in the warmest month (Group 1, collected in September) had the poorest storage quality, consistent with results that have been reported elsewhere and have been associated with the residual white blood cell count after leukoreduction (24). The observed intergroup differences were shown to be real and not an artifact as confirmed by standard biochemical reference measurements.…”

Section: Discussionsupporting

confidence: 88%

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Flow morphometry to assess the red blood cell storage lesion

Melzak

Janetzko

et al. 2017

Cytometry Pt A

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We present a novel automated system for morphology analysis of red blood cells (RBC) under flow. RBC concentrates collected by blood banks for transfusions are stored for periods of up to several weeks, during which time a number of changes occur, collectively termed the storage lesion. Typically the extent of hemolysis is the defining criterion to determine the acceptability of the RBCs for transfusions. Morphological changes are related with biochemical alteration during the storage of RBCs. The typical blood smear procedure for determining such changes is a labor-intensive and potentially biased manual process. The advantage of the flow morphometry system presented here is that it provides fully automated morphological classification of RBCs with large sample numbers in a short time. Our system uses a commercially available flow cell and flow conditions that prevent adhesion of RBCs, thus eliminating the need for blocking agents such as albumin that affect the distribution of cell shapes. Our morphometry results are validated by comparison with standard biochemical assays (hemolysis, ATP) for blood from 17 donors stored under blood bank conditions for 13 weeks. We show that the percentage of spherocytes present can be used to estimate the status of RBC concentrates. © 2017 International Society for Advancement of Cytometry.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 88%

Flow morphometry to assess the red blood cell storage lesion

Melzak

Janetzko

et al. 2017

Cytometry Pt A

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“…1). Also others found similar platelet content and mean platelet volume comparing BC‐derived and apheresis PCs [17,18] or similar platelet content in BC‐derived PCs [19,20] and similar number of leukocytes [21,22].…”

Section: Quality Control Data: Content Of Platelets and Other Componentsmentioning

confidence: 99%

Buffy-coat-derived pooled platelet concentrates and apheresis platelet concentrates: which product type should be preferred?

2010

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There is an ongoing debate whether platelet concentrates (PCs) prepared from either whole-blood donations or by plateletpheresis are superior. Usage of these two product types varies greatly between countries and individual institutions. Some use mainly apheresis PCs; others prefer pooled PCs which are produced from whole-blood donations. This review summarizes the existing information on these product types. In the first part data on quality, efficacy and safety are reviewed. It is important to note that the issue cannot be answered just by comparing 'the' apheresis platelet concentrate versus 'the' pooled platelet concentrate. Other factors which determine the quality of a product, e.g. residual leukocyte count, plasma content, additive solution or storage period may be even more important. The focus of the debate should be shifted. It is much more needed to further improve the overall quality of PCs and to optimize treatment of thrombocytopenic patients than to concentrate on a single-edged view on just the preparation method. In the second part of this review we compare the product types from the donor's point of view. If PCs which are equally safe and effective can be obtained by various methods, ethics and the safety of the healthy volunteer donor tips the scales. The decision on the use of a particular product type should take into account all aspects of efficacy, side effects and availability of the product as well as the donor's perspective and the commitment to maximize the use of the valuable whole-blood donation.

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“…In a recent report, it is described that universal WBC reduction in RCC failed up to 3% of the products [4]. Quality control was based on flow cytometric NC counting where all nuclear DNA signals are equated with WBCs [1].…”

Section: Discussionmentioning

confidence: 99%

Quality control of leucocyte‐reduced blood components: overestimation of WBC content due to nucleated red blood cells

Fischer¹,

Moog²,

Giers³

2011

Vox Sanguinis

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Most methods for quality control of white blood cell (WBC) depletion in blood products are based on flow cytometric techniques. Nearly all commercial kits are based on propidium iodide staining of the DNA and subsequently counting those DNA based events as residual WBC. Here, we could show that a substantial proportion of those events are derived from nucleated red blood cells and therefore not specific for WBCs (e.g. in erythrocyte products 30%). We developed a flow cytometric method for residual WBC counting applying simultaneous DNA- and WBC-specific surface staining to enable this.

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Quality of leucoreduced red blood cell concentrates: 5 years of follow‐up in France

Abstract: Our QC data suggest an overall compliance with the standard. Our data bank is useful to inform on the performance of leucoreduced RBC preparation processes carried out with market available devices.

Cited by 17 publications

References 6 publications

Flow morphometry to assess the red blood cell storage lesion

Flow morphometry to assess the red blood cell storage lesion

Buffy-coat-derived pooled platelet concentrates and apheresis platelet concentrates: which product type should be preferred?

Quality control of leucocyte‐reduced blood components: overestimation of WBC content due to nucleated red blood cells

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