Background: The number of studies to address the Quality of life (QOL) after a motorcycle accident in the context of developing countries is low. In this study, we investigated the QOL of injured motorcyclists up to three months after their accident and determining the associated factors.Methods: In the present study, we included 190 injured motorcyclists who admitted at two referral specialized hospitals (Emam Reza and Shohada) in Tabriz, between June 2018 and January 2019, and agreed to participate. All injured motorcyclists were contacted through the telephone one and 171 of them (90%) three months after their accident to complete an EQ-5D-3L questionnaire. The baseline measurements were gathered by using face to face interviews in the hospitals. The QOL score could vary between 1-3. The higher score showed a lower QOL. The Generalized Estimating Equation (GEE) models were used to determine the factors affecting the motorcyclists’ QOL. Results: The injured motorcyclist’s QOL was relatively better three months after the accident (mean ± Standard Deviation: 2.15 ± 0.65) in comparison with their status a month after the accident (mean ± SD: 1.78 ± 0.51) (p<0.001). The multivariable model showed that individuals whose pelvis (Coef: 0.29, (95% Confidence Intervals [CI]: 0.16, 0.42), P= 0.001) and knee (Coef: 0.26, (95% CI: 0.10, 0.42), P= 0.001) were injured, experienced a lower QOL. Also, those whose accident had happened in rainy weather experienced lower QOL (Coef: 0.33, (95% CI: 0.12, 0.53), P= 0.001). Those who were in an accident with a vehicle experienced a better QOL than others (Coef: -0.26, (95% CI: -0.43, -0.09), P=0.002).Conclusion: The assessment of three-month post-discharge showed that the QOL of the motorcyclists was better. It is recommended that by designing multi-year cohort studies, the QOL of motorcyclists be assessed for a longer time to identify the practical factors that improve the QOL of injured motorcyclists. In hospital discharge victims (especially for those with lower limb injuries such as injury to pelvis and knee, victims with Post-traumatic stress disorder, depression, and anxiety problems), social support, psychological support, financial support, and rehabilitation should be considered to improve the QOL.