Background:
Melasma, oxidative stress, tyrosinase, antioxidants, Homogenized skin tissues, hyperpigmentation disease.
Aims:
To compare the levels of oxidative stress and tyrosinase activity in melasma patients with
healthy volunteers.
Methods:
After written consent, 130 patients were enrolled in a case–control study. 65 cases were
of melasma disorder, and 65 were served as control. Homogenized skin tissues were taken and
used to estimate superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione
peroxidase (GPx) (antioxidants), malondialdehyde (MDA) and tyrosine hydroxylase
(TH).
Results:
Melasma patients had lower basal levels of systemic antioxidants than healthy subjects.
Tyrosinase activity was shown to be greater in lesional skin than in non-lesional skin. In controls,
there was a good positive relationship between TH and MDA and an excellent negative relationship
between GPx and GSH. In melasma patients, there were significant associations between
CAT, GPx, SOD and MDA.
Conclusions:
Increased oxidative stress may affect tyrosinase activity and eumelanin synthesis
via the anabolic pathway of melanin synthesis, according to our findings. In conclusion, we discovered
a negative relationship between antioxidants and tyrosinase activity.