Diamondback moth, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), is a vegetable pest of the genus Brassica worldwide. The development of new, safer bio insecticides with less negative impacts on human health, ora, fauna, and speci c to the target is needed to combat this pest, particularly in smallscale organic agriculture. In this sense, the e ciency of 7 plant species in the form of aqueous botanical extracts was evaluated regarding the bio insecticide effect, using three extraction methods (orbital agitation, decoction, and infusion). There was a difference between the treatments, highlighting the decoction of Couroupita guianensis, which presented the highest e ciency (39%) for second-instar larvae of P. xylostella, followed by infusions of Codiaeum variegatum and Ruta graveolens, both with a 29% e ciency rate. The 3 extraction methods were statistically different, with decoction and orbital agitation presenting the best results.