Quantification and biodistribution of iron oxide nanoparticles in the primary clearance organs of mice using T
            <sub>1</sub>
            contrast for heating

Zhang, Jinjin; Ring, Hattie L.; Hurley, Katie R.; Shao, Qi; Carlson, Cathy S.; Idiyatullin, Djaudat; Manuchehrabadi, Navid; Hoopes, P. Jack; Haynes, Christy L.; Bischof, John C.

doi:10.1002/mrm.26394

Cited by 37 publications

(55 citation statements)

References 39 publications

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“…Initial measurements were focused on the longitudinal relaxation rate constant R 1 (= 1/T 1 ) of a range of silica shell thicknesses at 1 mg Fe mL −1 in 1% agarose (Figure b). The R 1 values for all of the sIONPs are lower than those for uncoated EMG308 and msIONPs 23b. The decrease in R 1 can be attributed to reduced water accessibility to the core.…”

Section: Resultsmentioning

confidence: 79%

“…b) The longitudinal relaxation rate constant (R 1 ) at 9.4T of EMG308, msIONPs, and sIONPs with various shell thicknesses at 1 mg Fe mL −1 . For comparison, the R 1 at 1 mg Fe mL −1 is shown for EMG308 (sIONP with shell thickness = 0 nm) and msIONP 23b. Error bars indicate the standard deviation across the sample and are not visible because they are smaller than the circular marker shown.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, we also present, for the first time, a demonstration that an ex vivo rat kidney can be uniformly loaded and washed out with CPA and sIONPs. sIONP loading and washout were evaluated using microcomputed tomography (µCT) and magnetic resonance imaging (MRI) . In short, we demonstrate that sIONPs are an effective and scalable embodiment of IONPs for nanowarming use in organs.…”

Section: Introductionmentioning

confidence: 98%

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Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

et al. 2020

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Cryopreservation technology allows long‐term banking of biological systems. However, a major challenge to cryopreserving organs remains in the rewarming of large volumes (>3 mL), where mechanical stress and ice formation during convective warming cause severe damage. Nanowarming technology presents a promising solution to rewarm organs rapidly and uniformly via inductive heating of magnetic nanoparticles (IONPs) preloaded by perfusion into the organ vasculature. This use requires the IONPs to be produced at scale, heat quickly, be nontoxic, remain stable in cryoprotective agents (CPAs), and be washed out easily after nanowarming. Nanowarming of cells and blood vessels using a mesoporous silica‐coated iron oxide nanoparticle (msIONP) in VS55, a common CPA, has been previously demonstrated. However, production of msIONPs is a lengthy, multistep process and provides only mg Fe per batch. Here, a new microporous silica‐coated iron oxide nanoparticle (sIONP) that can be produced in as little as 1 d while scaling up to 1.4 g Fe per batch is presented. sIONP high heating, biocompatibility, and stability in VS55 is also verified, and the ability to perfusion load and washout sIONPs from a rat kidney as evidenced by advanced imaging and ICP‐OES is demonstrated.

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

et al. 2020

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“…Further, we showed that the addition of 1 to 10 mg Fe/mL VS55 had no further impact on viability ( P < 0.05). In addition, previous data demonstrated that the msIONPs are nontoxic when cultured with HDF over time at low concentration (0.07 mg Fe/mL) (19), and when injected at higher concentration (20 mg Fe/mL in 200 μL) intravenously into mice (31). This biocompatibility is due to both the mesoporous silica coating and the PEG bound to it as described previously (19).…”

Section: Discussionmentioning

confidence: 99%

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

et al. 2017

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Vitrification, a kinetic process of liquid solidification into glass, poses many potential benefits for tissue cryopreservation including indefinite storage, banking, and facilitation of tissue matching for transplantation. To date, however, successful rewarming of tissues vitrified in VS55, a cryoprotectant solution, can only be achieved by convective warming of small volumes on the order of one mL. Successful rewarming requires both uniform and fast rates in order to reduce thermal mechanical stress and cracks, and to prevent rewarming phase crystallization. Here we present a scalable nanowarming technology for 1 to 80 mL samples using radiofrequency-excited mesoporous silica coated iron oxide nanoparticles in VS55. Advanced imaging including Sweep Imaging with Fourier Transform and micro computed tomography were used to verify loading and unloading of VS55 and nanoparticles and successful vitrification of human dermal fibroblast cells, porcine arteries, and porcine aortic heart valve leaflet tissue. Nanowarming was then used to demonstrate uniform and rapid rewarming at > 130 °C/min in both physical (1 to 80 mL) and biological systems (1 to 50 mL). Nanowarming yielded viability that matched control and/or exceeded gold standard convective warming in 1 to 50 mL systems, and improved viability compared to slow warmed (crystallized) samples. Finally, biomechanical testing displayed no significant biomechanical property changes in blood vessel length or elastic modulus after nanowarming compared to untreated fresh control porcine arteries. In aggregate, these results demonstrate new physical and biological evidence that nanowarming can improve the outcome of vitrified cryogenic storage of tissues in larger sample volumes.

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“…However, coating the IONPs with mesoporous silica has been shown to improve stability [152, 153]. Moreover, this coating has demonstrated a high stability within biological environments and has maintained that stability within CPAs [154, 155]. …”

Section: Bioengineering Applicationsmentioning

confidence: 99%

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Taylor¹,

Weegman²,

Baicu³

et al. 2019

Transfus Med Hemother

135

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In this concept article, we outline a variety of new approaches that have been conceived to address some of the remaining challenges for developing improved methods of biopreservation. This recognizes a true renaissance and variety of complimentary, high-potential approaches leveraging inspiration by nature, nanotechnology, the thermodynamics of pressure, and several other key fields. Development of an organ and tissue supply chain that can meet the healthcare demands of the 21st century means overcoming twin challenges of (1) having enough of these lifesaving resources and (2) having the means to store and transport them for a variety of applications. Each has distinct but overlapping logistical limitations affecting transplantation, regenerative medicine, and drug discovery, with challenges shared among major areas of biomedicine including tissue engineering, trauma care, transfusion medicine, and biomedical research. There are several approaches to biopreservation, the optimum choice of which is dictated by the nature and complexity of the tissue and the required length of storage. Short-term hypothermic storage at temperatures a few degrees above the freezing point has provided the basis for nearly all methods of preserving tissues and solid organs that, to date, have proved refractory to cryopreservation techniques successfully developed for single-cell systems. In essence, these short-term techniques have been based on designing solutions for cellular protection against the effects of warm and cold ischemia and basically rely upon the protective effects of reduced temperatures brought about by Arrhenius kinetics of chemical reactions. However, further optimization of such preservation strategies is now seen to be restricted. Long-term preservation calls for much lower temperatures and requires the tissue to withstand the rigors of heat and mass transfer during protocols designed to optimize cooling and warming in the presence of cryoprotective agents. It is now accepted that with current methods of cryopreservation, uncontrolled ice formation in structured tissues and organs at subzero temperatures is the single most critical factor that severely restricts the extent to which tissues can survive procedures involving freezing and thawing. In recent years, this major problem has been effectively circumvented in some tissues by using ice-free cryopreservation techniques based upon vitrification. Nevertheless, despite these promising advances there remain several recognized hurdles to be overcome before deep-subzero cryopreservation, either by classic freezing and thawing or by vitrification, can provide the much-needed means for biobanking complex tissues and organs for extended periods of weeks, months, or even years. In many cases, the approaches outlined here, including new underexplored paradigms of high-subzero preservation, are novel and inspired by mechanisms of freeze tolerance, or freeze avoidance, in nature. Others apply new bioengineering techniques such as nanotechnology, isochoric pressure preserv...

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Quantification and biodistribution of iron oxide nanoparticles in the primary clearance organs of mice using T ₁ contrast for heating

Cited by 37 publications

References 39 publications

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

New Approaches to Cryopreservation of Cells, Tissues, and Organs

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Quantification and biodistribution of iron oxide nanoparticles in the primary clearance organs of mice using T 1 contrast for heating

Cited by 37 publications

References 39 publications

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Preparation of Scalable Silica‐Coated Iron Oxide Nanoparticles for Nanowarming

Improved tissue cryopreservation using inductive heating of magnetic nanoparticles

New Approaches to Cryopreservation of Cells, Tissues, and Organs

Contact Info

Product

Resources

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Quantification and biodistribution of iron oxide nanoparticles in the primary clearance organs of mice using T ₁ contrast for heating