Quantification and Determination of Stability of Tylvalosin in Pig Plasma by Ultra-High Liquid Chromatography with Ultraviolet Detection

Abstract

Tylvalosin (TV) is a macrolide antibiotic that is used for treating respiratory and enteric bacterial infections in swine and in poultry. In the coming years, the use of this drug will probably be widely studied in different species, but before its use in each veterinary species, macrolide analytical determination in various biological fluids is a pre-requisite step for the rational dose calculation of TV based on specific pharmacokinetic information. Its quantification is essential for detecting and avoiding the appearance of residues in animal products intended for human consumption. Therefore, a robust chromatographic method coupled with an ultraviolet detector was fully validated for the quantification of TV in pig plasma. A mixture (78:22) of (A) 0.3% formic acid in water and (B) acetonitrile was used as the mobile phase. TV and enrofloxacin (internal standard) were eluted at 14.1 and 5.9 min, respectively. Calibration curves ranged from 0.1 to 5 μg/mL. The accuracy and precision parameters for the quality controls were always <13.0%. Recovery ranged from 89.66 to 96.92%. The detection and quantification limits were found to be 0.05 μg/mL and 0.1 μg/mL, respectively. This method could be applied to develop pharmacokinetic studies.

Comparison of pharmacokinetics of two tylvalosin oral formulations in broiler chickens

“…Macrolides are an important group of antimicrobial agents targeting ribosomal synthesis in sensitive gram‐positive and some gram‐negative bacteria (Abo El‐Ela et al, 2015; Hernandis et al, 2022). Macrolides are widely used in veterinary medicine because of their safety, fast and wide tissue distribution after parenteral as well as oral administration (Hernandis et al, 2022; Kanoh & Rubin, 2010).…”

“…Macrolides are an important group of antimicrobial agents targeting ribosomal synthesis in sensitive gram‐positive and some gram‐negative bacteria (Abo El‐Ela et al, 2015; Hernandis et al, 2022). Macrolides are widely used in veterinary medicine because of their safety, fast and wide tissue distribution after parenteral as well as oral administration (Hernandis et al, 2022; Kanoh & Rubin, 2010). The newest member of macrolides tylvalosin, a derivative of tylosin, is synthesized by changing the 3‐acetyl‐40‐isovaleryl group to acetylisovaleryl tylosin tartrate group in tylosin (Elbadawy et al, 2019; Stuart et al, 2007; Zhao et al, 2014).…”

“…Macrolides are an important group of antimicrobial agents targeting ribosomal synthesis in sensitive gram-positive and some gramnegative bacteria (Abo El-Ela et al, 2015;Hernandis et al, 2022).…”

“…Macrolides are widely used in veterinary medicine because of their safety, fast and wide tissue distribution after parenteral as well as oral administration (Hernandis et al, 2022;Kanoh & Rubin, 2010).…”