2019
DOI: 10.1016/j.fshw.2019.09.002
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Quantification and discovery of PCR inhibitors found in food matrices commonly associated with foodborne viruses

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Cited by 23 publications
(18 citation statements)
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“…In animal species including humans, diagnostic methods, such as nucleic acid testing, allow for the direct detection of the microorganism causing the infection, typically after an amplification step using, preferentially but not exclusively, the polymerase chain reaction (PCR), whilst indirect methods, such as serological assays, allow for the detection of antibodies against microorganism antigens. Pathogen detection in plants that lack an immune system do rely on the proper detection of the pathogen, but with a couple of notable exceptions: the amplification of specific targets through PCR is often proven difficult due to the presence of inhibitors in plant cells ( Dorn et al, 1999 ; Schrader et al, 2012 ; Rački et al, 2014 ; Lacroix et al, 2016 ; Lardeux et al, 2016 ; Suther and Moore, 2019 ), whereas, the role of the detected pathogen component is rather obscure at least from the etiological point of view. In the case of viral plant pathogens, for instance, the capsid protein subunits or the virus particles containing the viral genome may be detected simultaneously ( Manoussopoulos and Tsagris, 2015 ), indicating the establishment of the virus in its host.…”
Section: Discussionmentioning
confidence: 99%
“…In animal species including humans, diagnostic methods, such as nucleic acid testing, allow for the direct detection of the microorganism causing the infection, typically after an amplification step using, preferentially but not exclusively, the polymerase chain reaction (PCR), whilst indirect methods, such as serological assays, allow for the detection of antibodies against microorganism antigens. Pathogen detection in plants that lack an immune system do rely on the proper detection of the pathogen, but with a couple of notable exceptions: the amplification of specific targets through PCR is often proven difficult due to the presence of inhibitors in plant cells ( Dorn et al, 1999 ; Schrader et al, 2012 ; Rački et al, 2014 ; Lacroix et al, 2016 ; Lardeux et al, 2016 ; Suther and Moore, 2019 ), whereas, the role of the detected pathogen component is rather obscure at least from the etiological point of view. In the case of viral plant pathogens, for instance, the capsid protein subunits or the virus particles containing the viral genome may be detected simultaneously ( Manoussopoulos and Tsagris, 2015 ), indicating the establishment of the virus in its host.…”
Section: Discussionmentioning
confidence: 99%
“… Reque et al (2014) also showed an increase in the activity of anthocyanins in frozen blueberries after 3 months of storage followed by a decrease during the subsequent 3 months. Lastly, the presence of pectin found in blueberries may also inhibit RT-qPCR ( Suther and Moore, 2019 ). These findings are consistent with the significant variations seen in our detections of HAV RNA at 60 and 90 days of storage.…”
Section: Discussionmentioning
confidence: 99%
“…Despite its advantages, saliva, like other biological samples, is prone to matrix-specific factors that influence diagnostics including intrinsic degrading enzymes, changes in salivary flow over time (e.g., circadian rhythms), or oral microbiome composition [8][9][10]12]. Moreover, various PCR inhibitors have been described in milk, vegetables, and foods high in protein and fat which interfere with robust diagnostic detection of pathogen nucleic acids [8,9]. Together with unique dietary habits of children, these factors pose challenges to molecular microbiology diagnostics and are essential to consider as we learn to exploit saliva for capturing infections with upper respiratory pathogens.…”
Section: Discussionmentioning
confidence: 99%
“…Despite its advantages, saliva, like other biological samples, is prone to matrix-specific factors that influence diagnostics including intrinsic degrading enzymes, changes in salivary flow over time (e.g., circadian rhythms), or oral microbiome composition [8][9][10]12]. Moreover, various PCR inhibitors have been described in milk, vegetables, and foods high in protein and fat which interfere with robust diagnostic detection of pathogen nucleic acids [8,9].…”
Section: Discussionmentioning
confidence: 99%
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