Quantification and Pathogenicity of Candida albicans in Denture-Wearing and Nondenture-Wearing Elderly

Abstract

Objective The primary purpose of this study was to evaluate and compare the microbial loads and pathogenicity traits of oral Candida albicans in denture-wearing (DW; n = 15) and nondenture-wearing (NDW; n = 15) elderly persons. Materials and Methods The fungal counts of the saliva, tongue dorsa, and prosthesis-fitting surfaces of the participants were assessed using real-time polymerase chain reaction to compare the quantity and expression of selected C. albicans biofilm-associated genes (ALS3, HWP1, and YWP1). Statistical Analysis The obtained data were analyzed by one-way analysis of variance, followed by Bartlett’s test. When appropriate, the Student’s t-test was also used; a value of p < 0.05 was considered statistically significant. Results In both groups, the count of C. albicans was found to be significantly higher in saliva than in other oral samples. The expression of the hypha-specific genes (ALS3 and HWP1) in the tongue dorsa was higher in the DW group (p < 0.05), whereas the transcription level of the yeast-specific gene (YWP1) was significantly higher in the NDW group. Conclusion Both tongue dorsa and dentures appear to be sharing factors that are important for C. albicans biofilm growth in abiotic and biotic oral surfaces of the elderly.

The Discrepancy between Clove and Non-Clove Cigarette Smoke-Promoted Candida albicans Biofilm Formation with Precoating RNA-aptamer

“…In this study, we used a clinical isolate that we collected previously from the denture surface of denture wearer subject 17 and selected by using CHROMAgar 18 , while the reference strain ( C. albicans ATCC 10231), which was used as a targeted aptamer ligand 16 , was needed for the evaluation and validation of this experimental study. Briefly, all microorganisms were taken from stock cultures frozen in 15% glycerol at -80°C and sub-cultured onto yeast peptone agar plates (1% yeast extract, 2% peptone, 2% glucose;YPD) or when indicated in yeast nitrogen base medium, supplemented with 50 mM glucose (YNB).…”

“…This was done to evaluate CSC's involvement in the transition C. albicans morphogenetic, from yeast or blastospore to hyphae form. All the procedures used as described in a previous study 17 . Briefly, RNA isolation, purification, and reverse transcription of cDNA were conducted using TRIzol™ Reagent (Invitrogen Life Technologies, Carlbad, California, United States) followed by reverse transcription using the TaqMan® Reverse Transcription Reagents (Applied Biosystems, Waltham, Massachusetts, USA).…”

“…The samples were collected, after informed consent was obtained from all the participants 17 , in accordance with the approved protocol of the Bioethics Committee of the Faculty of Dentistry, Universitas Indonesia (protocol number 020950818). The protocol conformed to the criteria of the Helsinki Declaration and the good clinical practical guidelines of the International Council on Harmonization.…”

The discrepancy between Clove and Non-Clove Cigarette Smoke-Promoted Candida albicans Biofilm Formation with precoating RNA-aptamer

“…This was done to evaluate CSC's involvement in the transition C. albicans morphogenetic, from yeast or blastospore to hyphae form. All the procedures used as described in a previous study 17 . Briefly, RNA isolation, purification, and reverse transcription of cDNA were conducted using TRIzol™ Reagent (Invitrogen Life Technologies, Carlbad, California, United States) followed by reverse transcription using the Taq-Man® Reverse Transcription Reagents (Applied Biosystems, Waltham, Massachusetts, USA).…”

The Discrepancy between Clove and Non-Clove Cigarette Smoke-Promoted Candida albicans Biofilm Formation with Precoating RNA-aptamer

“…This was done to evaluate CSC's involvement in the transition C. albicans morphogenetic, from yeast or blastospore to hyphae form. All the procedures used as described in a previous study 17 . Briefly, RNA isolation, purification, and reverse transcription of cDNA were conducted using TRIzol™ Reagent (Invitrogen Life Technologies, Carlbad, California, United States) followed by reverse transcription using the…”