Quantification of 4 antidepressants and a metabolite by LC–MS for therapeutic drug monitoring

Choong, Eva; Rudaz, Serge; Kottelat, Astrid; Haldemann, Sophie; Guillarme, Davy; Veuthey, Jean‐Luc; Eap, Chin B.

doi:10.1016/j.jchromb.2011.03.049

Cited by 22 publications

(14 citation statements)

References 34 publications

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“…Therefore, the chromatographic conditions were optimized at pH 8.1 using ammonium acetate as buffer, with molarity varying from 5 to 50 mM. A XBridge C18 column was chosen as analytical support, as different analytical methods were successfully developed on this column in our laboratory [44][45][46]. This approach allowed us to run different methods overnight on the same HPLC-MS equipped with a single column.…”

Section: Hplc-msmentioning

confidence: 99%

Simultaneous quantification of selective serotonin reuptake inhibitors and metabolites in human plasma by liquid chromatography–electrospray mass spectrometry for therapeutic drug monitoring

Ansermot

Brawand-Amey

Eap

2012

Journal of Chromatography B

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Section: Hplc-msmentioning

confidence: 99%

Simultaneous quantification of selective serotonin reuptake inhibitors and metabolites in human plasma by liquid chromatography–electrospray mass spectrometry for therapeutic drug monitoring

Ansermot

Brawand-Amey

Eap

2012

Journal of Chromatography B

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“…Various analytical methods have been reported in the literature to detect each medication separately with their metabolites and/or in combination with other medications in rat and human plasma as well as urine samples. These include gas chromatography (GC; da Fonseca et al ., ), capillary electrophoresis (CE; Deng et al ., ), thin‐layer chromatography (TLC; Soliman and Youssef, ), high‐performance liquid chromatography (HPLC; Belal et al ., ; Yeniceli and Dogrukol‐Ak, ; Suryakant and Sadhana, ), liquid chromatography–mass spectrometry (LC/MS; Zhou et al ., ; Raman et al ., ; Choong et al ., ), gas chromatography–mass spectrometry (GC/MS; Kakde et al ., ), liquid chromatography–tandem mass spectrometry (LC/MS/MS; Moreira et al ., ; Suresh et al ., ; Fisher et al ., ; Pan et al ., ; Parekh et al ., ) and ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC/MS/MS) (Li et al ., ; Tu et al ., ; Denooz et al ., ). However, no method has been reported to simultaneously determine BUP, ESC and QUE in plasma.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Park

Lee

et al. 2014

Biomedical Chromatography

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In the present study, an effective high performance liquid chromatography-tandem mass spectrometric (HPLC/MS/MS) method was developed and validated to simultaneously determine bupropion (BUP), quetiapine (QUE) and escitalopram (ESC) in human plasma using carbidopa as the internal standard. Chromatographic separation was achieved on a Waters Sun Fire C18 column using reversed-phase chromatography. The MS/MS experiment was performed in positive ion multiple reaction monitoring mode to produce product ions of m/z 240.3 → 184.2 for BUP, 384.2 → 253.1 for QUE, 325.3 → 109.3 for ESC and 227.2 → 181.2 for the internal standard. The method showed good linearity (R(2) ≥ 0.997), precision (relative standard deviation ≤7.5%), satisfactory intra- and interday accuracy (88.4-113.0%) and acceptable extraction recovery (87.2-115.0%), matrix effect (84.5.5-108.7%) and stability (92.3-103.5%). The method was successfully applied to determine the concentrations of BUP, QUE and ESC in human plasma samples.

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“…Thus, the plasma concentration of hydroxybupropion exceeds that of bupropion (Carroll et al, ; Hoiseth, Haslemo, Uthus, & Molden, ; Wille et al, ). Hydroxylation to the main and active metabolite hydroxybupropion (Choong et al, ; Laib et al, ) is mediated by CYP2B6 (Brunen et al, ; Wille et al, ). Therefore, poor metabolizers of CYP2B6 were associated with significantly lower hydroxybupropion concentrations in serum and consequently a higher risk of reduced clinical response (Hoiseth et al, ).…”

Section: Introductionmentioning

confidence: 99%

Determination of hydroxybupropion in human serum for routine therapeutic drug monitoring in psychiatry: A tool for dose‐individualization in treatment with bupropion

Scherf‐Clavel

Wahl

Menke

et al. 2019

Biomedical Chromatography

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Therapeutic drug monitoring (TDM) has become a clinical routine in psychiatry. Nevertheless, for bupropion there is only one method available that is suitable for routine use. However, it involves a complex sample clean-up. Owing to the instability of bupropion in serum, the main and active metabolite hydroxybupropion was chosen as the target substance. Therefore, a simple and robust high-performance liquid chromatography method for the quantification of hydroxybupropion in serum was developed and validated. A volume of 30 μL serum was used for easy sample clean-up, based on protein precipitation with acetonitrile followed by online solid-phase extraction. As hydroxybupropion was present in high serum concentrations, UV detection was possible. Owing to the commonly available instrumentation, the method could easily be integrated in routine TDM. The newly developed method was validated following the guidelines for bioanalytical method validation of the European Medicines Agency and US Food and Drug Administration. The lower limit of quantification was 100 ng/mL (0.391 μM) and linearity was shown between 100 and 2500 ng/mL. Intraday and interday precision ranged from 1.17 to 6.79% and from 6.07 to 9.41%, respectively. Intraday and interday accuracy ranged from 89.97 to 110.86% and from 95.05 to 101.2%. The method was shown to be selective, accurate and precise. Additionally, the method was successfully implemented in the therapeutic drug monitoring laboratory of the Department of Psychiatry, Psychosomatics and Psychotherapy at the University Hospital of Würzburg, Germany. Six months of routine analysis showed a rather low correlation between applied dose and serum concentration and therefore the necessity of TDM for dose-individualization in the treatment with bupropion.

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Quantification of 4 antidepressants and a metabolite by LC–MS for therapeutic drug monitoring

Cited by 22 publications

References 34 publications

Simultaneous quantification of selective serotonin reuptake inhibitors and metabolites in human plasma by liquid chromatography–electrospray mass spectrometry for therapeutic drug monitoring

Simultaneous quantification of selective serotonin reuptake inhibitors and metabolites in human plasma by liquid chromatography–electrospray mass spectrometry for therapeutic drug monitoring

Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Determination of hydroxybupropion in human serum for routine therapeutic drug monitoring in psychiatry: A tool for dose‐individualization in treatment with bupropion

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