Quantification of 4‐OH‐2,6‐Xylidine and its Conjugates in Human Urine Samples Utilising Microextraction in Packed Syringe On‐line with Liquid Chromatography and Electrospray Tandem Mass Spectrometry (MEPS‐LC‐MS/MS)

Abdel‐Rehim, Mohamed; Askemark, Yvonne; Norsten‐Höög, Carina; Pettersson, Karl‐Johan; Halldin, Magnus M.

doi:10.1080/10826070600864882

Cited by 40 publications

(17 citation statements)

References 14 publications

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“…Thus, a fully automated method using MEPS is a promising approach to reduce time and labor effort in sample preparation and analysis [20][21][22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Determination of organic priority pollutants and emerging compounds in wastewater and snow samples using multiresidue protocols on the basis of microextraction by packed sorbents coupled to large volume injection gas chromatography–mass spectrometry analysis

Prieto

Schrader

Moeder

2010

Journal of Chromatography A

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“…Thus, a fully automated method using MEPS is a promising approach to reduce time and labor effort in sample preparation and analysis [20][21][22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Determination of organic priority pollutants and emerging compounds in wastewater and snow samples using multiresidue protocols on the basis of microextraction by packed sorbents coupled to large volume injection gas chromatography–mass spectrometry analysis

Prieto

Schrader

Moeder

2010

Journal of Chromatography A

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“…MEPS can be connected on-line to a liquid chromatography (LC) or a gas chromatography (GC) equipment without any modification of the instrument. Any sorbent material such as silica-based (C2, C8, C18), strong cation exchange (SCX), or molecular imprinted polymers (MIPs) can be used as packing bed or as a coating [9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…The MEPS technique has been used to extract a wide range of analytes from biological samples such as plasma, urine, and blood [9][10][11][12][13][14][15][16][17][18][19][20]. A key factor in MEPS is that the solvent volume employed in the elution of analytes during the extraction process must be of a suitable order of magnitude to allow for direct injection into an LC or GC system.…”

Section: Introductionmentioning

confidence: 99%

Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection

Chaves

Leandro

Carris

et al. 2010

Journal of Chromatography B

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“…6 Several sorbent material such as silica based matrices (C2, C8, and C18), strong cation exchange, restricted access material (RAM), or molecular imprinted polymers (MIPs) can be employed. 7,8 The MEPS technique has been used to extract a wide range of analytes from different matrices, such as biological fluids (plasma, blood, and urine), [7][8][9][10][11][12][13][14][15][16][17][18][19][20] water, 21,22 hair 23 and wine. 24,25 In the areas of food analysis, several published applications describe MEPS for the analysis of aflatoxin B2 and M2 metabolite trace analysis in milk, mycotoxin trace analysis in cereal, atrazine in cereal, and sulfonamide trace analysis in meat.…”

mentioning

confidence: 99%

Microextraction in packed sorbent for determination of sulfonamides in egg samples by liquid chromatography and spectrophotometric detection

Salami¹,

Queiroz²

2011

J. Braz. Chem. Soc.

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O presente trabalho descreve a aplicação bem sucedida da microextração em sorvente empacotado e cromatografia líquida com detecção de arranjo de diodos (MEPS/LC-DAD) para a determinação simultânea das sulfonamidas (sulfacetamida, sulfadiazina, sulfatiazol, sulfametazina sulfametoxipiridazina e sulfametoxazol) em amostras de ovos. As variáveis MEPS (pH da amostra, número de ciclos aspirar/dispensar, força iônica e procedimento de dessorção) foram otimizados para aumentar a sensibilidade analítica do método. O método apresentou linearidade na faixa de concentração de 30 ng g -1 (limite de quantificação, LOQ) a 300 ng g -1. Este valor de LOQ é inferior ao limite máximo de resíduo (LMR) preconizado para sulfonamidas em amostras de ovos (100 µg kg -1 ). As taxas de recuperação foram adequadas para todos os analitos (> 94%), bem como os dados de precisão inter-ensaio, com coeficientes de variação inferiores a 10%. Com base na validação analítica, a metodologia MEPS/LC é adequada para a determinação de sulfonamidas em amostras de ovos.The present work describes a successful application of microextraction packed sorbent and liquid chromatography with diode array detection (MEPS/LC-DAD) for simultaneous determination of the sulfonamides (sulfacetamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamethoxypiridazine and sulfamethoxazole) in egg samples. The MEPS variables (pH of the sample, draw-eject cycles, ionic strength, and desorption procedure) were optimized, in order to improve the sensitivity of the proposed method. The method was shown to be linear at concentrations ranging from 30 ng g -1 (limit of quantification, LOQ) to 300 ng g -1 . This LOQ value is lower than those established as the maximum residue limit (MRL) for egg samples (100 µg kg -1 ). The accuracy values were adequate for all analytes (> 94%), as well as the inter-day precision data, with coefficient of variation lower than 10%. On the basis of analytical validation, the MEPS/LC methodology has been shown to be a promising alternative for analysis of sulfonamides in egg samples. Keywords: MEPS, sulfonamides, liquid chromatography, egg IntroductionSulfonamides (SAs) are a group of synthetic antibiotics that have played an important role as effective chemotherapeutics in bacterial and protozoan infections in veterinary medicine practice. Subtherapeutic doses of more than ten SAs are routinely used in food-producing animals for prophylactic and growth-promoting purposes. SAs residues could be potentially toxic to aquatic organisms and humans because of their carcinogenic potency and possible antibiotic resistance. [1][2][3][4] There are no sulfonamides approved for use in laying hens. The use of veterinary drugs for medicinal purposes in laying hens could result in violative drug residues in food meant for human consumption. Of all the marketed SAs, only sulfamethazine and sulfadimethoxine have been approved for use in chickens. Furthermore, this approval extends to broilers only, not laying hens. 4,5 To safeguard human health, many countries ...

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Quantification of 4‐OH‐2,6‐Xylidine and its Conjugates in Human Urine Samples Utilising Microextraction in Packed Syringe On‐line with Liquid Chromatography and Electrospray Tandem Mass Spectrometry (MEPS‐LC‐MS/MS)

Cited by 40 publications

References 14 publications

Determination of organic priority pollutants and emerging compounds in wastewater and snow samples using multiresidue protocols on the basis of microextraction by packed sorbents coupled to large volume injection gas chromatography–mass spectrometry analysis

Determination of organic priority pollutants and emerging compounds in wastewater and snow samples using multiresidue protocols on the basis of microextraction by packed sorbents coupled to large volume injection gas chromatography–mass spectrometry analysis

Microextraction in packed sorbent for analysis of antidepressants in human plasma by liquid chromatography and spectrophotometric detection

Microextraction in packed sorbent for determination of sulfonamides in egg samples by liquid chromatography and spectrophotometric detection

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