Quantification of 5‐aminolevulinic acid by <scp>CE</scp> using dynamic p<scp>H</scp> junction technique

Hsieh, Bo-Chuan; Chen, Richie L.C.; Tsai, Tsung‐Han

doi:10.1002/jssc.201200889

Cited by 5 publications

(4 citation statements)

References 50 publications

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“…In order to overcome this issue, the sample matrix conductivity was adjusted to be lower than that of the BGE. With this dilution, very good sensitivity improvement of about 50‐fold and an LOD of 1.0 mg/mL were achieved through prolonged sample injection up to 10.4% of capillary length .…”

Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

et al. 2015

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One of the most cited limitations of capillary (and microchip) electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of on-line/in-line concentration methods, covering the period July 2012-July 2014. It includes developments in the field of stacking, covering all methods from field-amplified sample stacking and large-volume sample stacking, through to ITP, dynamic pH junction, and sweeping. Attention is also given to on-line or in-line extraction methods that have been used for electrophoresis.

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Section: Stackingmentioning

confidence: 99%

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

et al. 2015

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“…A method for the analysis of 5‐aminolevulinic acid and its decomposition products in pharmaceutical sol‐gel formulations after aqueous extraction by CE was published by Hsieh et al. . Online dynamic pH junction preconcentration was performed by using 40 mM sodium borate buffer at pH 9.3 as the BGE and by applying the sample in a 40 mM sodium phosphate buffer matrix at pH 2.5.…”

Section: Ph‐mediated Stackingmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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“…In our work, a pH‐mediated sweeping technique was selected for the assay of low concentrations of MEL in real food samples, since MEL contains three amino groups and it could be strongly positively charged under acidic conditions and would be neutralized under neutral and alkaline condition. Several characteristics, such as sample pretreatment, injection ratio, buffer pH, concentration, stacking/separation voltages, etc., were carefully investigated and selected.…”

Section: Introductionmentioning

confidence: 99%

Assay of melamine in milk products with a pH‐mediated stacking technique in capillary electrophoresis

Yuan

Wang

et al. 2014

J of Separation Science

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A pH-mediated stacking method in capillary electrophoresis as an assay for low concentrations of melamine in milk products was established. Real samples were treated with acetone and sodium acetate and injected directly after centrifugation and filtration. Several experimental factors, such as buffer pH, buffer concentration, sample matrix, injection/sweeping ratio, sweeping time/voltages, separation voltages, as well as sample pretreatment, which affected stacking and separation, were investigated and optimized. Under the selected condition, a low LOD of 0.01 μmol/L (S/N = 5) and a wide range of linearity of 0.01∼1.0 μmol/L could be easily achieved with a good reproducibility (RSDs < 5.8% for both migration time and peak area) and an acceptable recovery of 94.0∼103.2% (for milk, infant formula, yogurt, and milk products). The proposed method was suitable for routine assay of melamine in real milk samples.

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Quantification of 5‐aminolevulinic acid by CE using dynamic pH junction technique

Cited by 5 publications

References 50 publications

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2012–2014)

Contemporary sample stacking in analytical electrophoresis

Assay of melamine in milk products with a pH‐mediated stacking technique in capillary electrophoresis

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