Quantification of a latex agglutination assay for bacterial pathogen detection in a low-cost capillary-driven fluidic platform

Pak, On Shun; Pietrzyk, Kyle; Ly, Andy; Maldonado-Liu, Andres; Fukuoka, Scott; Kim, Unyoung

doi:10.1109/ghtc.2016.7857364

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…In contrast, capillary-driven flow microfluidics has the potential to provide robust AMR devices for POC diagnostics. Several such studies were performed; Pak et al [ 64 ], for example, developed a latex agglutinating for the quantification of E. coli contamination levels in samples. In this study, agglutinates were formed via antigen binding to functionalized particles ( Figure 3 a).…”

Section: Tackling Antimicrobial Resistance In Capillary-driven Flomentioning

confidence: 99%

“…( a ) Immobilization of antibodies via latex agglutination test in which latex particles agglutinate upon the binding of the antibody with antigens loaded into the microchannel with the capillary flow ( A , B ). Reproduced with permission from [ 64 ]; ( b ) Fluoropolymer microcapillary-based identification of bacterial samples and antimicrobial susceptibility testing ( a – c ). Reproduced with permission from [ 16 ]; ( c ) Chip-and-dip capillary flow device fabrication and working schematic for beta-lactamase activity testing in microchannels ( a , b ).…”

Section: Figurementioning

confidence: 99%

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Capillary-Driven Flow Microfluidics Combined with Smartphone Detection: An Emerging Tool for Point-of-Care Diagnostics

et al. 2020

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Point-of-care (POC) or near-patient testing allows clinicians to accurately achieve real-time diagnostic results performed at or near to the patient site. The outlook of POC devices is to provide quicker analyses that can lead to well-informed clinical decisions and hence improve the health of patients at the point-of-need. Microfluidics plays an important role in the development of POC devices. However, requirements of handling expertise, pumping systems and complex fluidic controls make the technology unaffordable to the current healthcare systems in the world. In recent years, capillary-driven flow microfluidics has emerged as an attractive microfluidic-based technology to overcome these limitations by offering robust, cost-effective and simple-to-operate devices. The internal wall of the microchannels can be pre-coated with reagents, and by merely dipping the device into the patient sample, the sample can be loaded into the microchannel driven by capillary forces and can be detected via handheld or smartphone-based detectors. The capabilities of capillary-driven flow devices have not been fully exploited in developing POC diagnostics, especially for antimicrobial resistance studies in clinical settings. The purpose of this review is to open up this field of microfluidics to the ever-expanding microfluidic-based scientific community.

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Section: Tackling Antimicrobial Resistance In Capillary-driven Flomentioning

confidence: 99%

Section: Figurementioning

confidence: 99%