Quantification of a pegylated interferon-alpha2a product by a customised and validated reverse phase-high performance liquid chromatography method

Ghazaly, Maria El; Meager, Anthony; Zikry, Heidi; Ebaed, Mina; Shaker, Sami; Müeller, Frank; Rohde, Jan

doi:10.1016/j.jpba.2013.05.032

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…In recent years, liquid chromatography tandem mass spectrometry (LC-MS/MS) has been widely used in the quantification of therapeutic drugs, including PEGylated protein drugs. − Gong and his co-workers used LC-MS/MS coupled with in-source collision-induced dissociation to quantify PEG and PEGylated proteins . Thus, along this line, we hypothesize that PEG and PEGylated drugs without tags also can be exactly quantified via LC-MS/MS with in-quadrupole collision-induced dissociation (CID).…”

Section: Introductionmentioning

confidence: 96%

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

et al. 2018

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Fundamental studies on the cellular uptake and drug release of PEGylated nanomedicines are beneficial to understand their fate in vivo and construct ideal nanoparticle formulations. In this work, the detailed metabolic process of PEGylated doxorubicin (Dox) nanomedicines were investigated via confocal laser scanning microscopy (CLSM), flow cytometry (FCM), cytotoxicity test, fluorescence imaging in vivo (FLIV) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Among them, only LC-MS/MS could accurately determine the content of PEGylated Dox and Dox in vitro and in vivo. To the best of our knowledge, this was the first time the PEGylated Dox and released Dox were simultaneously quantified. The interplay of molecular structures, cellular uptake, drug release, and antitumor effect was well characterized. PEG with high molecular weight impeded the cellular uptake of nanoparticles, and the acid-labile hydrazone bond between Dox and PEG promoted Dox release significantly. Cellular uptake and drug release play decisive roles in cytotoxicity and antitumor effect, as evidenced by LC-MS/MS. We emphasized that LC-MS/MS would be a practicable method to quantify PEGylated drugs without complex tags, which could be more in-depth to understand the interaction between PEGylated nanomedicines and their antitumor efficacy.

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Section: Introductionmentioning

confidence: 96%

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

et al. 2018

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“…São descritas na literatura diversas técnicas de análise de IFN a partir de amostras puras, preparações farmacêuticas, e até mesmo de amostras de soro (CUETO-ROJAS et al, 2010;EL GHAZALY et al, 2013;SILVA et al, 2009;ZARRIN et al, 2006;ZIMMERMANN et al, 2013…”

Section: Produção De Ifn Em Escala Laboratorialunclassified

Desenvolvimento de processo de produção e caracterização de interferon-α2a secretado no espaço periplásmico de <i>Escherichia Coli</i>

Dias¹

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Aos meus pais e à minha irmã AGRADECIMENTOS A Deus por estar presente em todos os momentos da minha vida, por me mostrar que sempre posso ser uma pessoa melhor. Pelo amor incondicional. Aos meus pais e à minha irmã, por todos esses anos de ensinamentos do verdadeiro sentido da palavra amor. À toda a minha família pelo apoio, conselhos, suporte e carinho. Ao prof. Carlos pela oportunidade de desenvolver este trabalho. Por todo o apoio e (muita) paciência. Aos amigos do IPEN

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Assessment of the Degradation Pattern and Extent of PEG Interferon α-2b Using a Stability-Indicating SE-HPLC Assay

Abed¹,

Al‐Ghobashy

Fathalla³

et al. 2014

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Quantification of a pegylated interferon-alpha2a product by a customised and validated reverse phase-high performance liquid chromatography method

Cited by 9 publications

References 19 publications

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

Desenvolvimento de processo de produção e caracterização de interferon-α2a secretado no espaço periplásmico de <i>Escherichia Coli</i>

Assessment of the Degradation Pattern and Extent of PEG Interferon α-2b Using a Stability-Indicating SE-HPLC Assay

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Quantification of a pegylated interferon-alpha2a product by a customised and validated reverse phase-high performance liquid chromatography method

Cited by 9 publications

References 19 publications

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

The Effect of Molecular Structure on Cytotoxicity and Antitumor Activity of PEGylated Nanomedicines

Desenvolvimento de processo de produção e caracterização de interferon-&alpha;2a secretado no espaço periplásmico de <i>Escherichia Coli</i>

Assessment of the Degradation Pattern and Extent of PEG Interferon α-2b Using a Stability-Indicating SE-HPLC Assay

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Desenvolvimento de processo de produção e caracterização de interferon-α2a secretado no espaço periplásmico de <i>Escherichia Coli</i>