Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

Rodrigues, Lenize da Silva; Bovolato, Ana Lívia de Carvalho; Silva, Bárbara Esteves; Chizzolini, Leticia Victória; Cruz, Bianca Latance da; Moraes, Marcelo Padovani de Toledo; Lourenção, Pedro Luiz Toledo de Arruda; Bertanha, Matheus

doi:10.1590/acb361001

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…The most suitable scaffolds for tissue engineering of blood vessels should be able to withstand vascular stress without degeneration, do not promote thrombotic events, present good interaction with adjacent tissues and implanted cells, and enable nutrient transport through the porosity of their wall until they are fully developed [ 20 ]. More than one of our previous studies [ 15 ] have determined that the method used in the production of a biological scaffold produced by the decellularization of the vena cava in the rabbit model meets these requirements, and the current study demonstrates that the interaction with autologous ASCs promotes a favorable environment, generating the ideal adaptive conditions when it is implanted on the animal’s back.…”

Section: Discussionmentioning

confidence: 75%

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Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

et al. 2022

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Peripheral arterial disease (PAD) is the main cause of mortality in the western population and requires surgical intervention with the use of vascular substitutes, such as autologous veins or Dacron or PTFE prostheses. When this is not possible, it progresses to limb amputation. For cases where there is no autologous vascular substitute, tissue engineering with the production of neovessels may be a promising option. Previous experimental studies have shown in vitro that rabbit vena cava can be decellularized and serve as a scaffold for receiving mesenchymal stem cells (MSC), with subsequent differentiation into endothelial cells. The current study aimed to evaluate the behavior of a 3D product structure based on decellularized rabbit inferior vena cava (IVC) scaffolds seeded with adipose-tissue-derived stem cells (ASCs) and implanted in rabbits dorsally subcutaneously. We evaluated the induction of the inflammatory response in the animal. We found that stem cells were positive in reducing the inflammatory response induced by the decellularized scaffolds.

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Section: Discussionmentioning

confidence: 75%

“…Decellularization was carried out using the protocol with 1% SDS for 2 h under shaking in a Shaker (News Brunswick Scientific ® Edison, NJ, USA), at 37 °C. ( Supplementary file S1 ) [ 9 , 13 , 15 ]. The fragments were stored in a refrigerator at 4 °C in a sterile solution containing antibiotics and fungicide.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

et al. 2022

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“…When using biological scaffolds, the native architecture is highly preserved, and the antigen content is removed during the decellularization process [ 41 , 42 , 43 ]. The use of decellularized veins as biological scaffolds for tissue engineering in blood vessels was chosen as a biocompatible, resistant, and adaptable 3D tubular structure, which had good results in cell culture in experiments previously carried out by our study team [ 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Deciphering Key microRNA Regulated Pathways in Tissue-Engineered Blood Vessels: Implications for Vascular Scaffold Production

Rodrigues,

Felix,

Minutentag

et al. 2024

IJMS

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MicroRNAs (miRNAs) are non-coding RNAs involved in the regulation of gene expression associated with cell differentiation, proliferation, adhesion, and important biological functions such as inflammation. miRNAs play roles associated with the pathogenesis of chronic degenerative disorders including cardiovascular diseases. Understanding the influence of miRNAs and their target genes can effectively streamline the identification of key biologically active pathways that are important in the development of vascular grafts through the tissue engineering of blood vessels. To determine miRNA expression levels and identify miRNA target genes and pathways with biological roles in scaffolds that have been repopulated with adipose-derived stem cells (ASCs) generated through tissue engineering for the construction of blood vessels. miRNA quantification assays were performed in triplicate to determine miRNA expression in a total of 20 samples: five controls (natural inferior vena cava), five scaffolds recellularized with ASCs and differentiated into the endothelium (luminal layer), five samples of complete scaffolds seeded with ASCs differentiated into the endothelium (luminal layer) and smooth muscle (extraluminal layer), and five samples of ASC without cell differentiation. Several differentially expressed miRNAs were identified and predicted to modulate target genes with roles in key pathways associated with angiogenesis, vascular system control, and endothelial and smooth muscle regulation, including migration, proliferation, and growth. These findings underscore the involvement of these pathways in the regulatory mechanisms that are essential for vascular scaffold production through tissue engineering. Our research contributes to the knowledge of miRNA-regulated mechanisms, which may impact the design of vascular substitutes, and provide valuable insights for enhancing clinical practice. The molecular pathways regulated by miRNAs in tissue engineering of blood vessels (TEBV) allowed us to elucidate the main phenomena involved in cellular differentiation to constitute a blood vessel, with the main pathways being essential for angiogenesis, cellular differentiation, and differentiation into vascular smooth muscle.

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“…Furthermore, the physicochemical signals remain intact for regulating cell adhesion, proliferation, and differentiation, thus, providing a superior substrate for MSC seeding [ 7 ]. The scaffold produced through venous decellularization has biomechanical properties sufficient to resist the stresses of the urinary system and offers an extracellular matrix favorable to the cell population [ 12 , 13 , 14 ]. Tissue engineering research for the production and replacement of urinary bladder tissue has grown in recent decades; however, the clinical applications have not been completely established [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits

et al. 2022

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Background: To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces. Methods: New Zealand rabbits (n = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm2) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm2) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scanning electron microscopy (SEM). Results: The integrity of the urinary bladder was maintained in both groups. A superior regenerative process was observed in the G2 group, compared to the G1 group. We observed a greater urothelial epithelialization and maturity of the mucosa and submucosa fibroblasts. Furthermore, SEM demonstrated a notable amount of urothelial villus in the G2 group. Conclusion: Decellularized vena cava scaffolds were able to maintain the integrity of the urinary bladder in the proposed model. In addition, ASCs accelerated the regenerative process development, observed primarily by the new urothelial epithelization and the maturity of mucosa and submucosa fibroblasts.

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Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

Cited by 3 publications

References 26 publications

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits

Deciphering Key microRNA Regulated Pathways in Tissue-Engineered Blood Vessels: Implications for Vascular Scaffold Production

Urinary Bladder Patch Made with Decellularized Vein Scaffold Seeded with Adipose-Derived Mesenchymal Stem Cells: Model in Rabbits

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