Quantification of aflatoxin M1 in raw milk by a core-shell column on a conventional HPLC with large volume injection and step gradient elution

Mao, Jianfei; Lei, Shaorong; Liu, Yunhua; Xiao, Dan; Fu, Chaomei; Zhong, Lingli; Ouyang, Huaxue

doi:10.1016/j.foodcont.2014.11.022

Cited by 24 publications

(18 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[3][4][5] Once present in dairy products, AFM 1 poses a hazard to humans (especially infants) who consume them. 6 Taking this threat into consideration, many countries have set maximum limits for AFM 1 and established various regulations. 7,8 The European Union (EU) set a maximum tolerated level of AFM 1 to 0.050 μg/kg for adult consumption and subsequently more restrictively to 0.025 μg/kg for food for infants and children.…”

Section: Introductionmentioning

confidence: 99%

“…9 In China and the United States, the maximum limit for AFM 1 is 0.5 μg/kg in milk. 6,10 Hence, the development of simple, sensitive and selective methods to determine the presence and level of AFM 1 is much in demand for food safety organizations to implement regulatory requirements.…”

Section: Introductionmentioning

confidence: 99%

“…Approaches have been developed in recent years for quantitative determination of AFM 1 including high-performance liquid chromatography (HPLC) with fluorescence detection (FLD), 6,[11][12][13] high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS). 8,14,15 However, the quantitative approaches require complicated pretreatment, professional operators and expensive instruments.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

View full text Add to dashboard Cite

. In addition, the aptasensor developed here exhibits high selectivity for AFM 1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM 1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets. IntroductionAflatoxin M 1 (AFM 1 ), one of the most toxic contaminants in dairy products, is a metabolite produced by dairy cows as a result of being fed with feeds contaminated with Aflatoxin B 1 (AFB 1 ). [3][4][5] Once present in dairy products, AFM 1 poses a hazard to humans (especially infants) who consume them. Results and Discussion Optimization of the amplification of complementary ssDNAThe complementary ssDNA is applied as the subsequent PCR template. The Optimization of streptavidin and the biotinylated aptamerOther factors would affect the performance of this method including the concentrations of streptavidin, the biotinylated aptamer and the complementary ssDNA and these all should be optimized. Under a fixed concentration of complementary ssDNA at 10 nM, the concentrations of streptavidin and the 6 biotinylated aptamer were analyzed for the PCR amplification signal change (Fig. S3).The adsorptive power of streptavidin to PCR tubes and the binding ability of streptavidin-coated tubes to biotinylated aptamer was primarily detected using different concentrations of streptavidin (Fig. S3). This also shows that there was a clear difference of Ct values between the control group and streptavidin-coated tubes, which showed the intense adsorptive ability between biotinylated aptamer and streptavidin-coated tubes. In analysis of the Ct values at different concentrations of streptavidin, the Ct value reached the lowest level when the concentration of streptavidin was 2.5 ng mL -1 . This graph also shows that the Ct values decreased with the increase of the amount of aptamer when aptamer levels were below 10.0 nM andalso that the Ct values increased with the amount of aptamer above 10.0 nM mainly because of steric hindrance. Thus, 2.5 ng mL -1 of streptavidin and 10 nM of aptamer were the optimal conditions for RT-qPCR amplification. AFM 1 determinationUnder optimal conditions, the amplification curves and calibration curve of this aptasensor for different levels of AFM 1 with AFM 1 DNA1 were determined by RT-qPCR (Fig. 1). As is shown in Fig. 1(A), the cycle number increased with an increase in AFM 1 . More complementary ssDNA would be released when more AFM 1 is present in the reaction system, which leads to a decrease in the amount of the PCR template and an increase in cycle number. Meanwhile, a good linear relationshipbetween Ct values and AFM 1 levels in the range of 1×10 -4 to 1 µg L -1 was obtained as (Table 1). Specificity analysisThe specificity of the aptasensor plays an important role in the development and practicality of this method. In ...

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A qPCR aptasensor for sensitive detection of aflatoxin M1

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The same approach has been applied to the determination of Aflatoxin M1, a carcinogenic compound occurring in milk, in which EU limit is set at 0.05 mg/kg for adult consumption and was set more restrictively to 0.025 mg/kg for infants and young children, baby food products. This method has been optimized by the application of a large volume injection (100 μ L) and stepwise gradient elution that together with a sensitivity improvement contributed to fulfil strict legal requirements avoiding any solvent replacement [ 47 ].…”

Section: Applications In Food Analysismentioning

confidence: 99%

Core-Shell Columns in High-Performance Liquid Chromatography: Food Analysis Applications

Preti

2016

International Journal of Analytical Chemistry

View full text Add to dashboard Cite

The increased separation efficiency provided by the new technology of column packed with core-shell particles in high-performance liquid chromatography (HPLC) has resulted in their widespread diffusion in several analytical fields: from pharmaceutical, biological, environmental, and toxicological. The present paper presents their most recent applications in food analysis. Their use has proved to be particularly advantageous for the determination of compounds at trace levels or when a large amount of samples must be analyzed fast using reliable and solvent-saving apparatus. The literature hereby described shows how the outstanding performances provided by core-shell particles column on a traditional HPLC instruments are comparable to those obtained with a costly UHPLC instrumentation, making this novel column a promising key tool in food analysis.

show abstract

“…Confirmatory and quantitative approaches for the detection of mycotoxins mainly include thin layer chromatography (TLC) (Var et al, 2007), high-performance liquid chromatography (HPLC) (Herzallah, 2009;Wang et al, 2012;Yazdanpanah et al, 2013;Lee and Lee, 2015;Mao et al, 2015;Pietri et al, 2016), and liquid chromatography coupled with mass spectrometry (LC-MS) (Corcuera et al, 2011;Abia et al, 2013;Warth et al, 2013). However, expensive and special instruments, complicated pretreatment, and professional personnel are required for the use of these typical equipment methods (Shim et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Biosensor for Detection of Mycotoxins

et al. 2020

View full text Add to dashboard Cite

Mycotoxins are a large type of secondary metabolites produced by fungi that pose a great hazard to and cause toxic reactions in humans and animals. A majority of countries and regulators, such as the European Union, have established a series of requirements for their use, and they have also set maximum tolerance levels. The development of high sensitivity and a specific analytical platform for mycotoxins is much in demand to address new challenges for food safety worldwide. Due to the superiority of simple, rapid, and low-cost characteristics, aptamer-based biosensors have successfully been developed for the detection of various mycotoxins with high sensitivity and selectivity compared with traditional instrumental methods and immunological approaches. In this article, we discuss and analyze the development of aptasensors for mycotoxins determination in food and agricultural products over the last 11 years and cover the literatures from the first report in 2008 until the present time. In addition, challenges and future trends for the selection of aptamers toward various mycotoxins and aptasensors for multi-mycotoxins analyses are summarized. Given the promising development and potential application of aptasensors, future research studies made will witness the great practicality of using aptamer-based biosensors within the field of food safety.

show abstract

Quantification of aflatoxin M1 in raw milk by a core-shell column on a conventional HPLC with large volume injection and step gradient elution

Cited by 24 publications

References 38 publications

A qPCR aptasensor for sensitive detection of aflatoxin M1

A qPCR aptasensor for sensitive detection of aflatoxin M1

Core-Shell Columns in High-Performance Liquid Chromatography: Food Analysis Applications

Aptamer-Based Biosensor for Detection of Mycotoxins

Contact Info

Product

Resources

About