Quantification of antileukemic drug Dasatinib in human plasma: Application of a sensitive liquid chromatographic method

Pretreatment and analysis techniques development of TKIs in biological samples for pharmacokinetic studies and therapeutic drug monitoring

“…The parameter was optimized using a 3-level factorial design approach, and the best results were obtained under the following conditions: C 18 column, ammonium format buffer consisting of acetonitrile (28.19:71.81, V / V ), and buffer with pH 3. This optimized method was successfully applied to a pharmacokinetic study, demonstrating high recovery (99.91%), specificity (99.94%), precision (RSD < 0.543%), and robustness (RSD < 0.500%) ( Table S1 ) [ 2 , 3 , 5 , 8 , 12 , [15] , [16] , [17] , [26] , [27] , [28] , [29] , [30] , [31] , [32] , [33] , [34] , [35] , [37] , [38] , [39] , 41 , [48] , [49] , [50] , 52 , 54 , 55 , 61 , 63 , 64 , 76 , 80 , 83 , 84 , 86 , [88] , [89] , [90] , [91] , [92] , [93] , [94] , [95] , [97] , [98] , [99] , [100] , [101] , [102] , [103] , [104] , [105] , 109 , [111] , [112] , [113] , [114] ,…”

“…In the case of protein-rich materials, such as plasma and urine, it is necessary to employ different techniques for protein precipitation prior to analysis [ 26 , 27 ]. Typically, sample pre-purification involves a one-step simple protein precipitation using a protein precipitant followed by sample collection through centrifugation (∼15 min) [ 28 ], with a small volume of sample [ 29 ]. Commonly used protein precipitants include methanol, acetonitrile, dichloromethane, among others.…”