Quantification of biofilm produced by clinical, environment and hands’ isolates Klebsiella species using colorimetric and classical methods

Silva, Nagela Bernadelli Sousa; Alves, Priscila Guerino Vilela; Marques, Lara de Andrade; Silva, Samuel Fernandes; Faria, Gabriel de Oliveira; Araújo, Lúcio Borges de; Pedroso, Reginaldo dos Santos; Penatti, Mário Paulo Amante; Menezes, Ralciane de Paula; Röder, Denise Von Dolingër de Brito

doi:10.1016/j.mimet.2021.106231

Cited by 5 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the definition proposed in the protocol prepared by Stepanovic et al, biofilm-forming clinical strains are classified into four categories as strong biofilm producers, moderate biofilm producers, weak biofilm producers and non-biofilm producers [ 31 ]. The proposed criteria for classifying biofilm producers are also widely used by researchers working with K. pneumoniae strains [ 32 , 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation of Clinical Klebsiella pneumoniae Strains Isolated from Tracheostomy Tubes and Their Association with Antimicrobial Resistance, Virulence and Genetic Diversity

2021

View full text Add to dashboard Cite

(1) Background: Due to the commonness of tracheotomy procedures and the wide use of biomaterials in the form of tracheostomy tubes (TTs), the problem of biomaterial-associated infections (BAIs) is growing. Bacterial colonization of TTs results in the development of biofilms on the surface of biomaterials, which may contribute to the development of invasive infections in tracheostomized patients. (2) Methods: Clinical strains of K. pneumoniae, isolated from TTs, were characterized according to their ability to form biofilms, as well as their resistance to antibiotics, whether they harbored ESβL genes, the presence of selected virulence factors and genetic diversity. (3) Results: From 53 patients, K. pneumoniae were detected in 18 of the TTs examined, which constitued 34% of all analyzed biomaterials. Three of the strains (11%) were ESβL producers and all had genes encoding CTX-M-1, SHV and TEM enzymes. 44.4% of isolates were biofilm formers, SEM demonstrating that K. pneumoniae formed differential biofilms on the surface of polyethylene (PE) and polyvinyl chloride (PVC) TTs in vitro. A large range of variation in the share of fimbrial genes was observed. PFGE revealed sixteen genetically distinct profiles. (4) Conclusions: Proven susceptibility of TT biomaterials to colonization by K. pneumoniae means that the attention of research groups should be focused on achieving a better understanding of the bacterial pathogens that form biofilms on the surfaces of TTs. In addition, research efforts should be directed at the development of new biomaterials or the modification of existing materials, in order to prevent bacterial adhesion to their surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

Biofilm Formation of Clinical Klebsiella pneumoniae Strains Isolated from Tracheostomy Tubes and Their Association with Antimicrobial Resistance, Virulence and Genetic Diversity

2021

View full text Add to dashboard Cite

show abstract

“…Studies describe a vital virulence trait used by Klebsiella pneumoniae: the ability to form biofilms on biotic and abiotic surfaces [64][65][66][67][68][69][70][71][72]. There are two types of biofilms: hydrated biofilms (in drains and catheters) and dry surface biofilms (DSB) (on surfaces and some medical devices).…”

Section: Studies On Mdr Klebsiella Pneumoniae Biofilmmentioning

confidence: 99%

TiO2 Nanocomposite Coatings and Inactivation of Carbapenemase-Producing Klebsiella Pneumoniae Biofilm—Opportunities and Challenges

Bereanu,

Vintilă,

Bereanu

et al. 2024

Microorganisms

View full text Add to dashboard Cite

The worldwide increase of multidrug-resistant Gram-negative bacteria is a global threat. The emergence and global spread of Klebsiella pneumoniae carbapenemase- (KPC-) producing Klebsiella pneumoniae represent a particular concern. This pathogen has increased resistance and abilities to persist in human reservoirs, in hospital environments, on medical devices, and to generate biofilms. Mortality related to this microorganism is high among immunosuppressed oncological patients and those with multiple hospitalizations and an extended stay in intensive care. There is a severe threat posed by the ability of biofilms to grow and resist antibiotics. Various nanotechnology-based strategies have been studied and developed to prevent and combat serious health problems caused by biofilm infections. The aim of this review was to evaluate the implications of nanotechnology in eradicating biofilms with KPC-producing Klebsiella pneumoniae, one of the bacteria most frequently associated with nosocomial infections in intensive care units, including in our department, and to highlight studies presenting the potential applicability of TiO2 nanocomposite materials in hospital practice. We also described the frequency of the presence of bacterial biofilms on medical surfaces, devices, and equipment. TiO2 nanocomposite coatings are one of the best long-term options for antimicrobial efficacy due to their biocompatibility, stability, corrosion resistance, and low cost; they find their applicability in hospital practice due to their critical antimicrobial role for surfaces and orthopedic and dental implants. The International Agency for Research on Cancer has recently classified titanium dioxide nanoparticles (TiO2 NPs) as possibly carcinogenic. Currently, there is an interest in the ecological, non-toxic synthesis of TiO2 nanoparticles via biological methods. Biogenic, non-toxic nanoparticles have remarkable properties due to their biocompatibility, stability, and size. Few studies have mentioned the use of nanoparticle-coated surfaces as antibiofilm agents. A literature review was performed to identify publications related to KPC-producing Klebsiella pneumoniae biofilms and antimicrobial TiO2 photocatalytic nanocomposite coatings. There are few reviews on the antibacterial and antibiofilm applications of TiO2 photocatalytic nanocomposite coatings. TiO2 nanoparticles demonstrated marked antibiofilm activity, but being nano in size, these nanoparticles can penetrate cell membranes and may initiate cellular toxicity and genotoxicity. Biogenic TiO2 nanoparticles obtained via green, ecological technology have less applicability but are actively investigated.

show abstract

Reusable and non-invasive TiO2-based photodynamic transdermal patch (RPT) for treating MDR-negative bacteria strain and promote wound healing through a synergistic approach of ROS-induced RNS

Machina,

Parveen

et al. 2025

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

Quantification of biofilm produced by clinical, environment and hands’ isolates Klebsiella species using colorimetric and classical methods

Cited by 5 publications

References 20 publications

Biofilm Formation of Clinical Klebsiella pneumoniae Strains Isolated from Tracheostomy Tubes and Their Association with Antimicrobial Resistance, Virulence and Genetic Diversity

Biofilm Formation of Clinical Klebsiella pneumoniae Strains Isolated from Tracheostomy Tubes and Their Association with Antimicrobial Resistance, Virulence and Genetic Diversity

TiO2 Nanocomposite Coatings and Inactivation of Carbapenemase-Producing Klebsiella Pneumoniae Biofilm—Opportunities and Challenges

Reusable and non-invasive TiO2-based photodynamic transdermal patch (RPT) for treating MDR-negative bacteria strain and promote wound healing through a synergistic approach of ROS-induced RNS

Contact Info

Product

Resources

About