Quantification of capillary blood cell flow using reflectance confocal microscopy

Cinotti, Élisa; Gergelé, Laurent; Perrot, Jean; Dominé, Alexandre; Labeille, B.; Borelli, Primavera; Cambazard, F.

doi:10.1111/srt.12128

Cited by 27 publications

(21 citation statements)

References 13 publications

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“…In a selected capillary, the number of peaks of luminous intensity in the capillary area (corresponding to bright blood cells) was calculated by Image J Ò software (freeware downloaded at http://rsbweb.nih.gov/ij/). A good intra observer reproducibility of the measurement has been previously reported [16]. Flow was measured on 2 different capillaries and average.…”

Section: In Vivo Confocal Microscopy Measurementmentioning

confidence: 99%

“…Reactive hyperaemia can be assessed with NIRS technology with the recovery slope, measured after the release of the vascular occlusion [14]. IVCM is an emerging imaging technique for the assessment of skin microcirculatory blood flow [15,16]. Contrast in confocal imaging occurs by backscattering of a focused laser beam from different structures of the skin tissue and blood cells within dermal capillaries.…”

Section: Introductionmentioning

confidence: 99%

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The venous–arterial difference in CO2 should be interpreted with caution in case of respiratory alkalosis in healthy volunteers

Morel

Gergelé

Dominé

et al. 2016

J Clin Monit Comput

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The venous-arterial difference in CO (ΔCO) has been proposed as an index of the adequacy of tissue perfusion in shock states. We hypothesized that the variation in PaCO (hyper- or hypocapnia) could impact ΔCO, partly through microcirculation adaptations. Fifteen healthy males volunteered to participate. For hypocapnia condition (hCO), the subjects were asked to hyperventilate, while they were asked to breathe a gas mixture containing 8 % CO for hypercapnia condition (HCO). The 2 conditions were randomly assigned. Blood gases were measured at baseline before each condition, and after 5-7 min of either hCO or HCO condition. Microcirculation was assessed by the muscle reoxygenation slope measured with near infrared spectroscopy following a vascular occlusion test and by skin circulation with in vivo reflectance confocal microscopy. ΔCO was significantly increased with hCO while it tended to decrease with HCO (non-significant). HCO induced a moderate increase of the resaturation slope of NIRS oxygenation. Skin microcirculatory blood flow significantly dropped with hCO, while it remained unchanged with hypercapnia. Our results warrant cautious interpretation of ΔCO as an indicator of tissue perfusion during respiratory alkalosis.

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Section: In Vivo Confocal Microscopy Measurementmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The venous–arterial difference in CO2 should be interpreted with caution in case of respiratory alkalosis in healthy volunteers

Morel

Gergelé

Dominé

et al. 2016

J Clin Monit Comput

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show abstract

“…[1][2][3] The nail bed vascular architecture in red lunula and the respective blood flow can be better observed intraoperatively and in vivo using reflectance confocal microscopy. 6,7 In our study, mottled lunula (on naked eye examination) was visible as LLB on onychoscopy (9 50). Similar bands have been reported only once previously in the literature; Jakhar and Kaur reported them as an onychoscopic feature of nail dystrophy secondary to lichen striatus.…”

Section: Reportmentioning

confidence: 50%

Intraoperative onychoscopy and histopathological correlation of red lunula in nail lichen planus: a case series

Kaur

Jakhar

2020

Clin Exp Dermatol

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Summary The lunula is the white half‐moon‐shaped area located at the base of the fingernails and toenails, and is the only visible part of nail matrix. Red lunula can present as a complete form (the whole lunula is red), incomplete form (only the proximal lunula is red) or mottled form. Red lunula has been associated with a number of dermatological and systemic conditions. It is one of the less common manifestations of nail lichen planus (LP). However, the pathogenesis of the red lunula is not yet clear. We present a series of six patients with nail LP presenting with red lunula, and describe the findings of intraoperative onychoscopy and histopathology in an attempt to understand the pathogenesis underlying the condition.

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“…This operating wavelength of 830 nm is at an "optical window" of the skin (mean free path for scattering ~100 µm) and thus the system facilitates investigation of the epidermis and papillary dermis to a depth of ~350 µm with a lateral resolution of 0.4 µm and an axial resolution of 1.9 µm in the center of the image field. The laser power is relatively low at 30 mW, 7 therefore avoiding any tissue damage. An imaging frame rate of 9 fps is achievable with a field of view of 1000 x 1000 µm.…”

Section: Wide Field/confocal Microscopes and Oct Systemmentioning

confidence: 99%

“…4,5 More recently, reflectance-mode confocal microscopy has also been introduced to assess the human skin microcirculation. 6,7,8 Optical coherence tomography (OCT) is an emerging noninvasive imaging technique which can generate depth resolved images of the microcirculation 9,10,11 . However OCT itself does not directly produce these images of microcirculation.…”

Section: Introductionmentioning

confidence: 99%

In vivo correlation mapping microscopy

et al. 2016

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Abstract. To facilitate regular assessment of the microcirculation in-vivo, non-invasive imaging techniques such as nailfold capillaroscopy are required in clinics. Recently a correlation mapping technique has been applied to Optical Coherence Tomography (OCT) which extends the capabilities of OCT to microcirculation morphology imaging. This technique, known as cmOCT has been shown to extract parameters such as capillary density and vessel diameter, key clinical markers associated with early changes in microvascular diseases. However, OCT has limited spatial resolution in both the transverse and depth directions. Here, we extend this correlation mapping technique to other microscopy modalities, including confocal microscopy and take advantage of the higher spatial resolution offered by these modalities. The technique is achieved as a processing step on microscopy images and does not require any modification to the microscope hardware. Results are presented which show that this correlation mapping microscopy technique can extend the capabilities of conventional microscopy to enable mapping of vascular networks in-vivo with high spatial resolution in both the transverse and depth directions.

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Quantification of capillary blood cell flow using reflectance confocal microscopy

Cited by 27 publications

References 13 publications

The venous–arterial difference in CO2 should be interpreted with caution in case of respiratory alkalosis in healthy volunteers

The venous–arterial difference in CO2 should be interpreted with caution in case of respiratory alkalosis in healthy volunteers

Intraoperative onychoscopy and histopathological correlation of red lunula in nail lichen planus: a case series

In vivo correlation mapping microscopy

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