Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies

Alarcia-Lacalle, Ana; Barrasa, Helena; Maynar, Javier; Canut, Andrés; Gómez-González, Carmen; Solinís, Maria Ángelés; Isla, Arantxazu; Rodríguez-Gascón, Alicia

doi:10.3390/pharmaceutics13070959

Cited by 5 publications

(3 citation statements)

References 25 publications

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“…The HPLC system was controlled with the Waters Breeze HPLC software (version 6.20.00.00.; Waters, Milford, MA, USA). The quantification of ceftaroline was performed according to a previously validated method [19], and for avibactam we developed and validated a new method. Table 2 features the conditions for quantification.…”

Section: Methodsmentioning

confidence: 99%

Ceftaroline and Avibactam Removal by Continuous Renal Replacement Therapies: An in vitro Study

Alarcia-Lacalle¹,

Barrasa²,

Maynar³

et al. 2023

Blood Purif

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Introduction: Continuous renal replacement therapies (CRRTs) are frequently used in critically ill patients; however, there are scarce in vitro and in vivo studies showing the extracorporeal elimination of ceftaroline and avibactam. The aim of this study was to assess, through an in vitro model, the extracorporeal elimination of ceftaroline and avibactam by continuous veno-venous hemofiltration (CVVH), continuous veno-venous hemodiafiltration (CVVHDF), and continuous veno-venous hemodialysis (CVVHD), using a polysulfone hemofilter. Methods: Simulated in vitro experiments were performed using a multiFiltrate machine with a 1.4 m2 Ultraflux® AV600S polysulfone hemofilter. Isofundin® without or with bovine serum albumin was circulated as vehicle for ceftaroline or avibactam. Pre-filter, post-filter, and effluent samples were taken over a period of 60 min, and they were immediately stored at 4°C until processed in the same day. The quantification of ceftaroline and avibactam in the samples was performed by high-performance liquid chromatography with ultraviolet detection. Protein binding, extraction coefficient (EC), and extracorporeal clearance (CLCRRT) were calculated. Results: The elimination of both ceftaroline and avibactam during the three extracorporeal modalities followed first-order pharmacokinetics. Regardless of the CRRT technique, EC values for both molecules were around 1, similar to the unbound fraction of avibactam (0.96) and higher than the unbound fraction of ceftaroline (0.79). CLCRRT of ceftaroline ranged from 15.63 to 17.66 mL/min when CVVH and CVVHD were used with a flow rate of 1,000 mL/h, and from 29.25 to 32.95 mL/min for the CVVHDF modality with a flow rate of 2,000 mL/h. For avibactam, CLCRRT ranged from 15.07 to 18.82 mL/min for CVVH and CVVHD, and from 33.74 to 34.13 mL/min for CVVHDF. Discussion: Avibactam and ceftaroline are extensively removed through the polysulfone membrane, and a dose adjustment may be recommended for patients under CRRT to ensure pharmacodynamic target achievement.

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Section: Methodsmentioning

confidence: 99%

Ceftaroline and Avibactam Removal by Continuous Renal Replacement Therapies: An in vitro Study

Alarcia-Lacalle¹,

Barrasa²,

Maynar³

et al. 2023

Blood Purif

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“…Liquid chromatography methods coupled with mass–mass spectrometry (LC-MS/MS) or with UV detection (HPLC-UV) have been validated for the TDM of ceftaroline [ 35 , 36 ]. The clinical PK/PD targets for cephalosporin efficacy are set at 45–100%fT > MIC, based on the severity of the infection (MRSA vs. MSSA) and on the patients’ conditions (more aggressive targets should be considered in ICU settings) [ 37 ].…”

Section: β-Lactams: Fifth Generation Cephalosporinsmentioning

confidence: 99%

New Antimicrobials for Gram-Positive Sustained Infections: A Comprehensive Guide for Clinicians

Carcione,

Intra,

Andriani

et al. 2023

Pharmaceuticals

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Antibiotic resistance is a public health problem with increasingly alarming data being reported. Gram-positive bacteria are among the protagonists of severe nosocomial and community infections. The objective of this review is to conduct an extensive examination of emerging treatments for Gram-positive infections including ceftobiprole, ceftaroline, dalbavancin, oritavancin, omadacycline, tedizolid, and delafloxacin. From a methodological standpoint, a comprehensive analysis on clinical trials, molecular structure, mechanism of action, microbiological targeting, clinical use, pharmacokinetic/pharmacodynamic features, and potential for therapeutic drug monitoring will be addressed. Each antibiotic paragraph is divided into specialized microbiological, clinical, and pharmacological sections, including detailed and appropriate tables. A better understanding of the latest promising advances in the field of therapeutic options could lead to the development of a better approach in managing antimicrobial therapy for multidrug-resistant Gram-positive pathogens, which increasingly needs to be better stratified and targeted.

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“…El límite de cuantificación fue 0,5 mg/L. La curva de calibración fue linear en el rango de concentraciones de 0,5 a 100 mg/L, y la precisión y exactitud fueron ≤ 15% a altas, medias y bajas concentraciones y [ 3 ]…”

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Penetration in cerebrospinal fluid and use of ceftaroline in ventriculitis associated with external ventricular drainage

Ramos,

Benítez-Cano,

Luque

et al. 2024

Rev Esp Quimioter

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Quantification of Ceftaroline in Human Plasma Using High-Performance Liquid Chromatography with Ultraviolet Detection: Application to Pharmacokinetic Studies

Cited by 5 publications

References 25 publications

Ceftaroline and Avibactam Removal by Continuous Renal Replacement Therapies: An in vitro Study

Ceftaroline and Avibactam Removal by Continuous Renal Replacement Therapies: An in vitro Study

New Antimicrobials for Gram-Positive Sustained Infections: A Comprehensive Guide for Clinicians

Penetration in cerebrospinal fluid and use of ceftaroline in ventriculitis associated with external ventricular drainage

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