Quantification of corticosteroids in bovine urine using selective solid phase extraction and reversed-phase liquid chromatography/tandem mass spectrometry

Novel Fragmentation Pathways of Anionic Adducts of Steroids Formed by Electrospray Anion Attachment Involving Regioselective Attachment, Regiospecific Decompositions, Charge-Induced Pathways, and Ion–Dipole Complex Intermediates

“…From a mobile phase of acetonitrile/water/ formic acid (0.01 %), Pozo et al [41] reported [M+HCOO] when performing analysis of corticosteroid metabolites in urine. Tolgysea et al [42] documented that when acetate buffer is employed to adjust the pH in preparation for APCI-MS/MS of corticosteroids, more intense precursor ions were formed in the negative ion mode than in the positive ion mode. The formation of acetate adduct has been reported as common for anabolic steroids and corticosteroids, which have hydroxyl groups in the steroid skeleton [40].…”

Regioselective anion attachment leading to regiospecific decompositions of bifunctional steroids in negative ion electrospray tandem mass spectrometry

“…In addition, acetate adducts have also been observed when acetate buffer was employed in LC/APCI-MS. [27] The formation of acetate adducts of anabolic steroids and corticosteroids has been linked to the presence of hydroxyl substituents on the steroid backbone. [27,28] In this study, we have used a variety of anions (including A À ¼ fluoride, bicarbonate, acetate, and chloride) to generate anionic adduct signals,…”

Evaluation of Molecularly Imprinted Polymers for the Simultaneous SPE of Six Corticosteroids in Milk

“…Corticosteroids are steroid hormones which are known to reduce inflammation, as well as to suppress allergic reactions and immune system activity [1]. They have many physiological functions but they are mainly employed as antipyretic, anti-inflammatory and anti-allergy drugs [2] in both human and veterinary medicine.…”

“…Gas chromatography-mass spectrometry (GC-MS) [8,9], liquid chromatography-mass spectrometry (LC-MS/MS) [4,10] and methods based on immunoaffinity chromatography followed by reverse-phase highperformance liquid chromatography (IAC-HPLC) with diode array detection (DAD) [2] have been developed to identify corticosteroids in biological samples. In recent years, liquid chromatography coupled to different massspectrometry detectors has become a frequently used to quantitatively determine corticosteroids in complex matrices [10][11][12].…”