Quantification of CRP in human serum using a handheld fluorescence detection system for capillary-based ELISA

Morioka, Kazuhiro; Sato, Hiroto; Kuboyama, Minori; Yanagida, Akio; Shoji, Atsushi

doi:10.1016/j.talanta.2020.121725

Cited by 20 publications

(6 citation statements)

References 25 publications

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“…Currently, the detection limit of CRP in conventional enzyme-linked immunosorbent assay (ELISA) can reach the level of 0.1 ng/mL. , There are several innovative methods developed for the detection of CRP with varying degrees of sensitivity. , The plasmonic ELISA utilizes etched Ag-coated Au nanobipyramids for multicolorimetric detection and achieves an LOD of 0.09 ng/mL . The fluorescent fullerene nanoparticle-based LFIA can detect CRP concentrations between 0.1 and 10 ng/mL in serum in just 15 min .…”

Section: Resultsmentioning

confidence: 99%

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Lin,

Chen,

Huang

et al. 2024

ACS Sens.

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Surface-enhanced Raman scattering (SERS) technology has been widely recognized for its remarkable sensitivity in biochip development. This study presents a novel sandwich immunoassay that synergizes SERS with magnetoplasmonic nanoparticles (MPNs) to improve sensitivity. By taking advantage of the unique magnetism of these nanoparticles, we further enhance the detection sensitivity of SERS biochips through the applied magnetic field. Despite the high sensitivity, practical applications of SERS biochips are often limited by the issues of stability and reproducibility. In this study, we introduced a straightforward statistical method known as "Gaussian binning", which involves initially binning the two-dimensional Raman mapping data and subsequently applying Gaussian fitting. This approach enables a more consistent and reliable interpretation of data by reducing the variability inherent in Raman signal measurements. Based on our method, the biochip, targeting for C-reactive protein (CRP), achieves an impressive detection limit of 5.96 fg/mL, and with the application of a 3700 G magnetic field, it further enhances the detection limit by 5.7 times, reaching 1.05 fg/mL. Furthermore, this highly sensitive and magnetically tunable SERS biochip is easily designed for versatile adaptability, enabling the detection of other proteins. We believe that this innovation holds promise in enhancing the clinical applicability of SERS biochips.

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Section: Resultsmentioning

confidence: 99%

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Lin,

Chen,

Huang

et al. 2024

ACS Sens.

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“…Each of the five subunits is similar to a discoid orientation toward a central pore folded into two antiparallel two-layered β -sheets [ 32 ]. Native CRP (nCRP) dissociates to monomeric/modified isoform of CRP (mCRP) across lysophosphatidylcholine in platelets, apoptotic monocytic THP-1, and Jurkat T cells [ 33 , 34 ]. Moreover, a study by Ji et al demonstrates that nCRP, when bound to the cell membrane, dissociates into subunits while retaining some native conformation before entirely dissociating into mCRP subunits, which detaches from the membrane [ 35 ].…”

Section: Crpmentioning

confidence: 99%

“…Immunoturbidimetry is the most commonly used method for clinical CRP determination [ 58 , 59 ]. Conventional enzyme-linked immunosorbent assay (ELISA) and fluorescence-linked immunosorbent assay (FLISA) have also been widely used for the quantification of CRP [ 33 , 60 ]. The main limitations of these methods are the use of complex features and the lack of cost-effectiveness.…”

Section: Crpmentioning

confidence: 99%

Role of C-Reactive Protein in Diabetic Inflammation

Stanimirović

Radovanović

Banjac

et al. 2022

Mediators of Inflammation

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Even though type 2 diabetes mellitus (T2DM) represents a worldwide chronic health issue that affects about 462 million people, specific underlying determinants of insulin resistance (IR) and impaired insulin secretion are still unknown. There is growing evidence that chronic subclinical inflammation is a triggering factor in the origin of T2DM. Increased C-reactive protein (CRP) levels have been linked to excess body weight since adipocytes produce tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), which are pivotal factors for CRP stimulation. Furthermore, it is known that hepatocytes produce relatively low rates of CRP in physiological conditions compared to T2DM patients, in which elevated levels of inflammatory markers are reported, including CRP. CRP also participates in endothelial dysfunction, the production of vasodilators, and vascular remodeling, and increased CRP level is closely associated with vascular system pathology and metabolic syndrome. In addition, insulin-based therapies may alter CRP levels in T2DM. Therefore, determining and clarifying the underlying CRP mechanism of T2DM is imperative for novel preventive and diagnostic procedures. Overall, CRP is one of the possible targets for T2DM progression and understanding the connection between insulin and inflammation may be helpful in clinical treatment and prevention approaches.

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“…Furthermore, a tiny change of blood CRP concentration may portend chronic diseases, such as cardiovascular diseases, peripheral vascular diseases, and neonatal sepsis; therefore, high-sensitivity CRP (hs-CRP) detection at a level of 1 ng/mL is demanded [ 3 , 4 ]. Clinically, CRP is detected by the enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay, exhibiting high accuracy and sensitivity [ 5 , 6 ] but requiring professional operators and long testing time. The ideal hs-CRP detection should be implemented outside of central labs with features of quantification, rapidity, user friendliness, and low cost [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection

Jin

Zhang

et al. 2022

Biosensors

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Inflammation caused by viral or bacterial infection is a major threat to human health globally. Blood C-reactive protein (CRP) has been proven to be a sensitive indicator for the occurrence and development of inflammation. Furthermore, a tiny change of blood CRP concentration may portend chronic diseases; therefore, high-sensitivity CRP (hs-CRP) detection in a quantitative, rapid, user-friendly, and low-cost manner is highly demanded. In this paper, we developed a europium-chelate polystyrene microsphere (EuPSM)-based lateral flow immunoassay (LFIA) integrating with a benchtop fluorescence analyzer for hs-CRP detection. The optimization of the EuPSM-based LFIA was implemented through adjusting the antibody density on EuPSM from 100% to 60% of the saturated density. Finally, the limit of detection of 0.76 pg/mL and detection range of 0.025–250 ng/mL were obtained. Moreover, the clinical application capability of the proposed platform was validated through detecting CRP in clinical serum samples, showing high consistency with the results obtained from the clinical standard method. Hence, the proposed EuPSM-based LFIA has been verified to be well suitable for hs-CRP detection, while also showing great applicability for sensitively and rapidly detecting other biomarkers.

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Quantification of CRP in human serum using a handheld fluorescence detection system for capillary-based ELISA

Cited by 20 publications

References 25 publications

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Improving Sensitivity and Reproducibility of Surface-Enhanced Raman Scattering Biochips Utilizing Magnetoplasmonic Nanoparticles and Statistical Methods

Role of C-Reactive Protein in Diabetic Inflammation

Eu-Chelate Polystyrene Microsphere-Based Lateral Flow Immunoassay Platform for hs-CRP Detection

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