Quantification of cutaneous mast cells using morphometric point counting and a conjugated avidin stain

Kasper, Candace S.; Tharp, Michael D.

doi:10.1016/s0190-9622(87)70044-9

Cited by 37 publications

(22 citation statements)

References 12 publications

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“…16 In another report, 2 which included 12 canine mast cell tumors, both avidin peroxidase and Giemsa stained tumors. Therefore, the authors concluded that avidin peroxidase was no better than Giemsa.…”

Section: Discussionmentioning

confidence: 99%

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Simões

Schoning

1994

J VET Diagn Invest

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Abstract. Twelve histochemical methods; affinity staining with avidin peroxidase, wheat germ agglutinin, and concavalin-A agglutinin; and an immunohistochemical stain with Kpl (CD68) antibody were compared for their relative effectiveness in staining canine mast cell tumors. Stains were compared in 28 mast cell tumors and 19 histiocytomas. The effectiveness of the histochemical methods and the lectins decreased as the mast cells became less differentiated. None of the staining methods were positive on histiocytomas. Periodic acidSchiff (PAS) gave positive results in a few cases of mast cell tumors where other histochemical stains were negative. Although avidin peroxidase and Kpl antibody stained more mast cell tumors than any other method, they did not differ significantly from Luna's method, toluidine blue pH 0.5, toluidine blue pH 4.5, alcian blue pH 2.5, safranin O, Unna's method, and Giemsa. No stain was ideal for the diagnosis of canine mast cell tumors; however, this study suggests that the use of avidin peroxidase, Kpl antibody, and PAS may give additional information for individual poorly differentiated tumors without substantial increase in time or cost.

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Section: Discussionmentioning

confidence: 99%

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Simões

Schoning

1994

J VET Diagn Invest

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“…Mast cells aggregate around blood vessels, and are sometimes associated with eosinophils. [19] In nodular forms of UP and mastocytomas, mast cells may infiltrate the entire dermis and extend into the subcutaneous tissues. Electron microscopy of the mast cells shows round and spindle-shaped cells that stain with both tryptase and chymase, sometimes in sheet-like distribution.…”

Section: Classification Of Cutaneous Mastocytosis In Childrenmentioning

confidence: 99%

Diagnosis and Treatment of Cutaneous Mastocytosis in Children

Castells

Metcalfe

Escribano

2011

American Journal of Clinical Dermatology

183

240

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Mastocytosis is a disease with many variants, all of which are characterized by a pathologic increase in mast cells in cutaneous tissue and extracutaneous organs such as the bone marrow, liver, spleen and lymph nodes. The disease presents in two primary age-related patterns: pediatric-onset mastocytosis and adult-onset mastocytosis, which may differ in their clinical manifestations and disease course. Pediatric-onset mastocytosis commonly is diagnosed prior to 2 years of age, and usually consists of cutaneous disease, with urticaria pigmentosa (UP) the most common pattern. The course of pediatric-onset mastocytosis is variable. This is in contrast to adult onset disease which generally presents with systemic findings and increases in extent and severity over time. Because pediatric forms of mastocytosis often differ in presentation and prognosis from adult variants, it is most important to understand pediatric mastocytosis and not rely on adult approaches as a guide on how to identify and manage disease. This is especially important in selecting therapy where antiproliferative agents have a very different set of concerns when used to treat adult mastocytosis compared to pediatric mastocytosis, especially in terms of long-term toxicity. This review is directed at providing age-specific information surrounding the care of the child with mastocytosis.

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“…sia, samples of neonatal foreskin were surgically removed from 3 newborns and a 1-year-old infant. JNormal adult skin was obtained from the trunk area of 2 adult cadaver donors (age 65 and 66) and an adult volunteer (age 36) as described previously (16). Diagnostic truncal cutaneous biopsies were removed following local anesthesia from 2 patients with systemic mastocytosis (ages 28 and 65), a patient with a basal cell carcinoma (BCC) (age 42) and a patient with a hemangioma (age 36).…”

Section: Methodsmentioning

confidence: 99%

“…Although these observations have been informative, they have been primarily descriptive in nature. As a result of our interest in mast cells from normal human skin as well as from pathologic cutaneous lesions (12)(13)(14)(15)(16), we have been intrigued by apparent variations in mast cell morphology under different conditions. In an attempt to quantitate these subjective morph-78 ologic differences, we have combined the methods of morphometrie analysis and electron microscopy for the assessment of human cutaneous mast cells.…”

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confidence: 99%

Ultrastructural morphometric analysis of human mast cells in normal skin and pathological cutaneous lesions

1988

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Electron micrographs of human mast cells in normal neonatal and adult skin and in cutaneous lesions of basal cell carcinoma (BCC), hemangioma and mastocytosis were assessed by morphometric analysis. Using this quantitative histologic approach, adult skin mast cells were found to be significantly larger (47.7 microns 2 +/- 2.4 SEM vs. 38.3 microns 2 +/- 1.8 SEM, p less than or equal to 0.001) and have larger granules (0.63 micron +/- .02 SEM vs. 0.53 micron +/- .02 SEM, p less than or equal to 0.001) than infant mast cells while both mast cell populations had comparable nuclear sizes (13.7 microns 2 +/- 0.9 SEM vs. 14.3 microns 2 +/- 0.8 SEM) and numbers of cytoplasmic granules (72 +/- 4.0 SEM vs. 66 +/- 4.0 SEM). Morphometric analysis of mast cell infiltrates in the adult skin lesions of BCC and hemangioma revealed that these cells were larger than neonatal mast cells but were similar to normal adult controls. Cutaneous mast cells from 2 mastocytosis patients, however, had significantly larger mean cell surface areas (78.0 microns 2 +/- 3.4 SEM and 70.6 microns 2 +/- 3.2 SEM, p less than or equal to 0.001), nuclear areas (20.8 microns 2 +/- 1.1 SEM and 21.3 microns 2 +/- 1.2 SEM p less than or equal to 0.001) and granule diameters (0.82 micron +/- 0.4 SEM and 0.83 micron +/- .03 SEM, p less than or equal to 0.001) when compared with mast cells in normal adult skin and in the other pathologic lesions. No difference in the total number of cytoplasmic granules was observed in the different mast cell populations.(ABSTRACT TRUNCATED AT 250 WORDS)

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Quantification of cutaneous mast cells using morphometric point counting and a conjugated avidin stain

Cited by 37 publications

References 12 publications

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Canine Mast Cell Tumors: A Comparison of Staining Techniques

Diagnosis and Treatment of Cutaneous Mastocytosis in Children

Ultrastructural morphometric analysis of human mast cells in normal skin and pathological cutaneous lesions

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