Quantification of Epstein-Barr Virus and Human Cytomegalovirus in Chronic Periodontal Patients

Abstract

Background:Although studies focused mainly on the identification of periopathogenic bacteria, recent reports have suggested that various herpes viruses may also be involved in the occurrence and progression of different forms of periodontal diseases.Objectives:This study aimed to compare the prevalence and load of Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV) in subgingival tissue specimens between chronic periodontitis and healthy sites.Patients and Methods:A total of 60 samples from the systematically healthy patients with chronic periodontitis participated in this study (mean age, 35 ± 7). Clinical periodontal evaluation included the plaque index (PI) (Loe and Silness), bleeding on probing (BOP) (O’Leary), bleeding index, periodontal pocket depth (PPD) and clinical attachment level measurement. Tissue specimens harvested from > 6 mm periodontal pockets and from ≤ 3 mm sulcus depth in a quadrant of the same patient using periodontal curettes. Moreover, the unstimulated whole saliva was gathered as a shedding medium. A Taq-man Real-Time Polymerase Chain Reaction assay was used to identify genomic copies of periodontal HCMV and EBV. Data were analyzed by the Wilcoxon-signed ranks and Friedman tests using the SPSS 16 software.Results:Out of 60 samples of subgingival tissues taken from the patients with chronic periodontitis, EBV count was the highest in saliva and the least in the tissue sample with PD < 3 mm (P < 0.05). The highest HCMV count was in saliva and tissue samples with PD > 6 mm (P < 0.05).Conclusions:According to the results of this study, quantification of HCMV and EBV observed in this study is high in periodontal tissue samples of severe chronic periodontitis.

Occurrence and quantification of Anelloviruses and Herpesviruses in gingival tissue in Chinese Shanghai sub-population

“…Moreover, the EBV virus loads were significantly higher in the AP and CP groups ( P < 0.05). These results are consistent with earlier reports about EBV in periodontitis [ 6 , 9 , 26 , 29 , 30 ]. However, the occurrence of HCMV was 5.3 and 11.9% in the AP and CP groups in this study, lower than what many previous studies have reported [ 31 – 33 ].…”

Is the gingival sulcus a potential niche for SARS-Corona virus-2?

“…Gingival sulcus has also been implicated as a favored site for the proliferation of pathogenic viruses like Human papillomavirus and Human herpesviruses 6 and 7 [9][10][11][12]. Symbiotic relationship between gingival microbes could be a potential reason for the high viral proliferation in the gingival sulcus.…”

Detection of EBV, CMV and HSV-1 in subgingival samples of HIV positive and negative patients with chronic periodontitis.

“…However, in the last decade the presence of herpesviruses has been shown, including HSV1 and 2, EBV, and CMV in periodontal pockets of patients with periodontitis [16][17][18][19][20][21][22] .…”