2001
DOI: 10.1038/sj.onc.1204917
|View full text |Cite
|
Sign up to set email alerts
|

Quantification of estrogen receptor α and β expression in sporadic breast cancer

Abstract: The recent cloning of a second estrogen receptor (ER), designated ERb, has prompted a reevaluation of the role of ERs in breast cancer. We have developed and validated a real-time RT ± PCR assay to quantify ERa and ERb gene expression at the mRNA level in a series of 131 patients with unilateral invasive primary breast cancer. Although ERb expression showed wide variations in tumor tissues, its range (nearly three orders of magnitude) was smaller than that of ERa (nearly four orders of magnitude), suggesting t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

10
101
0

Year Published

2002
2002
2023
2023

Publication Types

Select...
10

Relationship

6
4

Authors

Journals

citations
Cited by 123 publications
(111 citation statements)
references
References 20 publications
10
101
0
Order By: Relevance
“…The malignancy of infiltrating carcinomas was scored according to Scarff Bloom Richardson (SBR) histoprognostic system. Estrogen receptor (ERa), progesterone receptor (PR), and human EGF receptor 2 (ERBB2) status was determined at the protein level by using biochemical methods [dextran-coated charcoal method, enzyme immunoassay, or immunohistochemistry (IHC)] and confirmed by real-time quantitative RT-PCR assays (14,15 Supplementary Table S1. Patients (n ¼ 169) developed metastasis during a median follow-up of 8.9 years (range, 6 months to 29 years).…”
Section: Patients and Samplesmentioning
confidence: 99%
“…The malignancy of infiltrating carcinomas was scored according to Scarff Bloom Richardson (SBR) histoprognostic system. Estrogen receptor (ERa), progesterone receptor (PR), and human EGF receptor 2 (ERBB2) status was determined at the protein level by using biochemical methods [dextran-coated charcoal method, enzyme immunoassay, or immunohistochemistry (IHC)] and confirmed by real-time quantitative RT-PCR assays (14,15 Supplementary Table S1. Patients (n ¼ 169) developed metastasis during a median follow-up of 8.9 years (range, 6 months to 29 years).…”
Section: Patients and Samplesmentioning
confidence: 99%
“…Data analysis Real-time PCR was analysed using relative expression levels (Bieche et al, 2001). Expression of the genes was compared to both an endogenous RNA control, 18S RNA, and to levels in wild-type spleen as a tissue control for the tumors, similar to the method described by Bieche et al (2001 …”
Section: Real-time Pcr Analysismentioning
confidence: 99%
“…Primer sequences used to amplify human porphobilinogen deaminase (PBGD) (20), prostate-specific antigen (PSA) (21), ABCA1 (20), ABCG1 (22), LDLR (22), and HMGCR (22) cDNA have been previously described. Primer sequences for human SREBP-1c and SREBP-2 were provided by Dr. Etienne Lefai (Faculté de Médecine Lyon Sud).…”
mentioning
confidence: 99%