Quantification of estrogenic mycotoxins at the ng/L level in aqueous environmental samples using deuterated internal standards

Hartmann, Niccolo; Erbs, Marianne; Wettstein, Felix E.; Schwarzenbach, René P.; Bucheli, Thomas D.

doi:10.1016/j.chroma.2006.10.045

Cited by 73 publications

(70 citation statements)

References 23 publications

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“…36 α-zearalonol and β-zearalonol were identified in the urine of pasture-raised cattle and attributed to f usarium mold occurring in grasses. 37 In this study, the occurrence of α-zearalanol could be attributed to excretion from implanted cattle, or also due metabolism of zearalenone produced by fusarium mold which is commonly found in fermented corn 38,39 (SI Figure S6). In the current study, attribution of the α-zearalanol identified in manure, feedlot soils and runoff to the implant is difficult without additional information regarding α-zearalanol metabolism within the animal as well as information on processes controlling environmental transformation of naturally occurring mycotoxins.…”

Section: Environmental Science and Technologymentioning

confidence: 81%

Effect of Growth Promotants on the Occurrence of Endogenous and Synthetic Steroid Hormones on Feedlot Soils and in Runoff from Beef Cattle Feeding Operations

Bartelt–Hunt

Snow

Kranz

et al. 2012

Environ. Sci. Technol.

133

110

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Supplements and growth promotants containing steroid hormones are routinely administered to beef cattle to improve feeding efficiency, reduce behavioral problems, and enhance production. As a result, beef cattle manure will contain both synthetic steroids as well as a range of endogenous steroids including androgens, estrogens, and progestogens. A two-year controlled study was conducted in which beef cattle were administered steroid hormones via subcutaneous implants and feed additives and the occurrence of 16 endogenous and synthetic steroid hormones and metabolites was evaluated in runoff from beef cattle feedlots and in manure and soil collected from feedlot surfaces. Samples were extracted and analyzed using liquid chromatography tandem mass spectrometry for metabolites of the synthetic androgen trenbolone acetate, 17α-trenbolone, 17β-trenbolone, for the nonsteroidal semisynthetic estrogen agonist, α-zearalanol, and the synthetic progesterone melengesterol acetate, as well as a wide range of endogeneous estrogens, androgens, and f usarium metabolites. Synthetic steroids including trenbolone metabolites and melengestrol acetate were detected in fresh manure and in feedlot surface soils from cattle administered synthetic steroids at concentrations up to 55 ± 22 ng/g dry weight (dw) (17α-trenbolone) and 6.5 ± 0.4 ng/g dw (melengesterol acetate). Melengesterol acetate was detected in 6% of runoff samples from feedlots holding cattle administered synthetic steroids at concentrations ranging up to 115 ng/L. The presence of melengesterol acetate in runoff from beef cattle feeding operations has not been previously reported. Synthetic steroids were not detected in manure or runoff from control cattle. A wide range of endogenous hormones were detected in runoff and feedlot surface soils and manure from cattle given synthetic steroids and from control cattle, with no statistically significant differences in concentration. These results indicate that runoff from confined animal production facilities is of environmental and public health concern regardless of the use of growth promotants.

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Section: Environmental Science and Technologymentioning

confidence: 81%

Effect of Growth Promotants on the Occurrence of Endogenous and Synthetic Steroid Hormones on Feedlot Soils and in Runoff from Beef Cattle Feeding Operations

Bartelt–Hunt

Snow

Kranz

et al. 2012

Environ. Sci. Technol.

133

110

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“…Analysis of zearalenone was performed as described Hartmann et al (23). Deoxynivalenol was separated from other trichothecenes on a 50 × 2.0 mm i.d., 3 µm Polaris C18 A column (Varian, Inc., Walnut Creek, CA) at room temperature by applying the following elution gradient: 0 min, 5% B (95% A); 1 min, 5% B; 4 min, 30% B; 5 min, 100% B; 7 min, 100% B; 7.5 min, 5% B; and 15 min, 5% B; with eluent A consisting of Milli-Q water/ methanol (95:5, v/v) and eluent B of Milli-Q water/methanol (5:95, v/v).…”

Section: Methodsmentioning

confidence: 99%

“…Data processing was carried out using the software Varian MS Workstation (version 6.8, SP1). Analytical quality control parameters for deoxynivalenol in drainage and river water (ion suppression, absolute and relative recoveries, method precision method detection limits, and instrument linearity) were determined as described for zearalenone (23).…”

Section: Methodsmentioning

confidence: 99%

Fusarium Mycotoxins: Overlooked Aquatic Micropollutants?

Bucheli¹,

Wettstein²,

Hartmann³

et al. 2008

J. Agric. Food Chem.

Self Cite

112

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Deoxynivalenol and zearalenone are among the most prevalent toxins produced by Fusarium spp. They have been investigated in food and feed products for decades but rarely in the environment. We therefore established solid-phase extraction and liquid chromatography-mass spectrometry (LC-MS) methods to quantify these mycotoxins at trace concentrations in aqueous natural samples. In a model emission study, we inoculated a winter wheat field with Fusarium graminearum and subsequently monitored deoxynivalenol and zearalenone in its drainage water. Before during and after harvest in June and July 2007, these toxins were emitted in concentrations from 23 ng/L to 4.9 µg/L for deoxynivalenol and from not detected to 35 ng/L for zearalenone. Simultaneously, in July and August 2007, deoxynivalenol was also detected in a number of Swiss rivers in concentrations up to 22 ng/L and zearalenone was present in several river samples below the method quantification limit. Other mycotoxins might be emitted from Fusarium-infected fields as well, because some of them are produced in similar amounts as deoxynivalenol and zearalenone and exhibit similar or even higher water solubility than deoxynivalenol. The ecotoxicological consequences of the presence of mycotoxins in surface waters remain to be elucidated.

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“…ZEA pollution of the aquatic ecosystems has repeatedly been repeatedly confi rmed during the past decade (9,14,16,(27)(28)(29)(30)(31). However, no information is available on the pathways of toxin transport to surface waters.…”

Section: Zearalenone In Cereal Samplesmentioning

confidence: 99%

“…However, no information is available on the pathways of toxin transport to surface waters. According to Hartmann et al (27), the occurrence of mycotoxins in the aquatic environment is a consequence of surface run-off from cropped fi elds. Furthermore, mycotoxin producers may also develop in water (32)(33)(34).…”

Section: Zearalenone In Cereal Samplesmentioning

confidence: 99%

Zearalenone Contamination of the Aquatic Environment as a Result of its Presence in Crops / Pojava Mikotoksina U Vodenom Okolišu Zbog Njihove Prisutnosti U Usjevima

Waśkiewicz

Gromadzka

Bocianowski

et al. 2012

Archives of Industrial Hygiene and Toxicology

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The aim of this study was to establish a relation between zearalenone contamination of crops in the Polish province of Wielkopolska and its occurrence in aquatic ecosystems close by the crop fi elds. Water samples were collected from water bodies such as drainage ditches, wells, or watercourses located in four agricultural areas. Moreover, control water samples were collected from the Bogdanka river, which was located outside the agricultural areas and near an urban area. Cereal samples were collected in the harvest season from each agricultural area close to tested water bodies. Zearalenone (ZEA) was found in all water and cereal samples. The highest concentrations were recorded in the postharvest season (September to October) and the lowest in the winter and spring. Mean ZEA concentrations in water ranged between 1.0 ng L -1 and 80.6 ng L -1 , and in cereals from 3.72 ng g -1 to 28.97 ng g -1 . Our results confi rm that mycotoxins are transported to aquatic systems by rain water through soil.

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Quantification of estrogenic mycotoxins at the ng/L level in aqueous environmental samples using deuterated internal standards

Cited by 73 publications

References 23 publications

Effect of Growth Promotants on the Occurrence of Endogenous and Synthetic Steroid Hormones on Feedlot Soils and in Runoff from Beef Cattle Feeding Operations

Effect of Growth Promotants on the Occurrence of Endogenous and Synthetic Steroid Hormones on Feedlot Soils and in Runoff from Beef Cattle Feeding Operations

Fusarium Mycotoxins: Overlooked Aquatic Micropollutants?

Zearalenone Contamination of the Aquatic Environment as a Result of its Presence in Crops / Pojava Mikotoksina U Vodenom Okolišu Zbog Njihove Prisutnosti U Usjevima

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