Quantification of Exemestane Accumulation During Microbial Bioconversion by TLC Image Analysis

Patil, Prachi; Sharma, Rajesh; Banerjee, Tushar; Patil, Sandeep

doi:10.22159/ijpps.2017v9i8.19201

Cited by 2 publications

(2 citation statements)

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“…1 It is known to be a major reason for mortality throughout the world. [3][4][5][6] Several glycosides such as malvidin-3-b-glycoside, delphinidin-3-bglycoside, anthocyanins, ternatins (C1-C5 and O3) and preternatins (A3 and A4) have been reported to exist in the plant. In the current era, many plants have been documented to treat cancer; for instance vinca alkaloids are used against leukemia, Podophyllum is used for small lung and testicular cancer, Taxus spp.…”

Section: Introductionmentioning

confidence: 99%

“…It belongs to the Fabaceae family with common names such as anesthetic and sedative, hypoglycemic, anthelmintic, anxiolytic, antidote, hypoglycemic and anticancer properties. [3][4][5][6] Several glycosides such as malvidin-3-b-glycoside, delphinidin-3-bglycoside, anthocyanins, ternatins (C1-C5 and O3) and preternatins (A3 and A4) have been reported to exist in the plant. The owers of Ct have also been reported to contain many therapeutic bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

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A novel bioassay based gold nanoribbon biosensor to aid the preclinical evaluation of anticancer properties

et al. 2016

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In this work, we report a microbial biosensor fabricated for the preclinical assay of anticancer compounds.Gold nanoribbons were used as a transducer for mounting the microbe. For the synthesis of these unique Au nanostructures, quercetin stabilized gold nanoparticles (Q-AuNPs) were synthesized as a first step using onion peel. Later, dityrosine peptide was used as a sacrificial template for the synthesis of the gold nanoribbons (AuNRs). The structural morphology of the as-synthesized Au nanomaterial was examined using UV spectroscopy, XRD, SEM and TEM. The AuNRs were found to be <10 nm in diameter, which provided a good biocompatible environment and effective protection for the immobilization of Agrobacterium tumefaciens (At), a causative agent of crown gall disease. At is reported to cause tumors in plants through a tumorigenic mechanism similar to that of humans. Inhibition of At indicates that the inhibitory compound being screened exhibits anticancer activity. Clitoria ternatea (Ct) is traditionally used to cure many diseases and is known to possess anticancer activity. Therefore, we have used a Ct flower extract in the preclinical study of its anticancer activity against At by fabricating a simple electrochemical sensor. We have employed electrochemical techniques such as CV and EIS for the characterization of the developed microbial biosensor. Moreover, the as-synthesized AuNRs behave as an ideal transducer and platform, thus improving the electrode surface area and providing good biocompatibility for the immobilization of At. In contrast to other immobilization techniques and biosensors that often require elaborate procedures, cross-linking agents and rigorous chemical reactions, At was directly adsorbed onto the electrode under optimum conditions without any mediators. The results show that the developed biosensor is useful in the pre-clinical analysis of anticancer properties. Indeed the study examines the use of electrochemistry, demonstrating the rapid response and high sensitivity of the proposed sensor in contrast to bioassay procedures. In conclusion, the experimental results indicate that the developed biosensor accentuates the excellent properties of the synthesized AuNRs, which promises to be a novel avenue in designing biosensors.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel bioassay based gold nanoribbon biosensor to aid the preclinical evaluation of anticancer properties

et al. 2016

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A Facile, Efficient and Catalyst Free Synthesis of Imidazole, Tetrazole and Pyrimidine Combined Moiety as Potential Antimicrobial and Antitubercular Agents

Joshi¹,

Panchani²

2021

HETEROCYCLES

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The tetrazole fused pyrimidine system possesses a broad spectrum of biological activities. So, we have synthesized 5-(substitutedphenyl)-7-(1Himidazol-4-yl)-4,7-dihydrotetrazolo [1,5-a]pyrimidine derivatives (5a-n) by reaction of chalcones with 5-aminotetrazole without catalyst in appropriate solvent. The structural elucidation of these compounds is based on MS, IR, 1 H-NMR and 13 C-NMR spectral data. The in vitro anti-microbial activity was investigated against Gram-positive and Gram-negative bacterial and fungal strains.It was found that the compounds 5a, 5b, 5c, 5d, 5e showed significant activities against tested organisms as compared to standard drugs (Ampicillin and Griseofulvin) while compounds 5a, 5c, 5i, and 5k showed good percentage of average inhibition in the dormant and active stage of tuberculosis.We carried out the synthesis of tetrazolo [1,5-a]pyrimidines in the acidic and basic catalytic amount at reflux temperature, but there was not much satisfied with the yield and reaction time of obtained products.Again the same reaction was carried out without any solvent or catalyst in fused condition but yield was very low. So from the above conclusion, we have chosen, an alcoholic solvents such as methanol, n-propanol, isopropanol, n-butanol and polar solvent like dimethylformamide (DMF) and 1,4-dioxane.After doing various optimizations in the experimental conditions, it was concluded that, when the reaction was carried out in n-butanol solvent showed better yield (87%) in less reaction time.All the newly synthesized compounds were characterized using IR, 1 H-NMR, 13 C-NMR, and mass spectroscopy. The IR spectrum of synthesized compounds exhibited characteristic absorption band at 3075 cm -1 for aromatic C=C stretching vibration band. Characteristic absorption band observed at 1315 cm -1 for C-N stretching in imidazole ring and at 1605 for C=N of the pyrimidine ring system and all other general frequency band are in good agreement with the structure. 1 H-NMR spectra of the compound 5b showed characteristic singlet signals at 12.16 ppm due to the proton of imidazole-NH and at 10.44 ppm due to the proton of fused pyrimidine-NH. Chiral carbon proton was observed doublet at 5.28 ppm with 2.80 Hz coupling constant value. The 1 H-NMR spectrum of compound 5c exhibited two doublets, arising from the two equivalent phenyl ring protons at 7.54 and 7.24 ppm. The 13 C-NMR spectrum of compound 5c showed signal at 53.23 ppm for the chiral carbon while at 96.00 ppm for the pyrimidine ring C=C carbon and at 21.12 ppm for the methyl carbon attached to phenyl ring. Molecular ion peak was observed in agreement with molecular weight of respective compound i.e. the molecular formula of compounds 5c and 5m by displaying molecular ion peaks at m/z 279 (M + ) and 325 (M + ) respectively. Scheme 1 PLAUSIBLE REACTION MECHANISM: Scheme 2The examination of the data of the Table 2 revealed that many of the compounds shown good activity against bacterial strain when compared with the standard drugs like Ampicillin, Chloramphenicol, and...

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Quantification of Exemestane Accumulation During Microbial Bioconversion by TLC Image Analysis

Cited by 2 publications

References 22 publications

A novel bioassay based gold nanoribbon biosensor to aid the preclinical evaluation of anticancer properties

A novel bioassay based gold nanoribbon biosensor to aid the preclinical evaluation of anticancer properties

A Facile, Efficient and Catalyst Free Synthesis of Imidazole, Tetrazole and Pyrimidine Combined Moiety as Potential Antimicrobial and Antitubercular Agents

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