Quantification of fenoprofen in human plasma using <scp>UHPLC</scp>–tandem mass spectrometry for pharmacokinetic study in healthy subjects

Bharwad, Kirtikumar D.; Shah, Priyanka A.; Shrivastav, Pranav S.; Sharma, Vinay S.; Singhal, Puran

doi:10.1002/bmc.4708

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…The method was validated per the US FDA guidelines (FDA, 2018) and was similar to our previous report (Bharwad, Shah, Shrivastav, Sharma, & Singhal, 2020).…”

Section: Methodsmentioning

confidence: 75%

Selective quantification of lacosamide in human plasma using UPLC–MS/MS: Application to pharmacokinetic study in healthy subjects with different doses

Bharwad

Shah

Shrivastav

et al. 2020

Biomedical Chromatography

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A practical, sensitive, and robust UPLC-MS/MS method was developed and validated to quantify lacosamide in human plasma. A simple one-step protein precipitation was used to extract lacosamide and labeled lacosamide-13C, D3 as an internal standard (IS) from 150-μL plasma. The extracts were analyzed on an Eclipse Plus C18 column (50 × 2.1 mm, 1.8 μm) using 0.1% formic acid in water and methanol:acetonitrile (50:50, v/v) under gradient conditions. The extracts were quantified on LCMS-8040 using electrospray ionization source operated in positive ionization and multiple reaction monitoring modes. The method showed good linearity from 0.02 to 20 μg/mL, which was adequate to cover lacosamide concentration assayed in formulations with different strengths. The bioanalytical assay was fully validated as per current regulatory guidelines. The intra-batch and inter-batch precision values of lacosamide were less than 4.6%. Lacosamide was found to be stable at different storage conditions. The extraction recoveries and IS-normalized matrix factors for lacosamide ranged from 97.17 to 99.68% and from 0.973 to 1.012, respectively. The validated method was successfully applied to a pharmacokinetic study with three lacosamide formulations (50, 100, and 200 mg) in 36 healthy subjects. The assay reliability was determined by reanalysis of 81 subject samples.

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“…The method was validated per the US FDA guidelines (FDA, 2018) and was similar to our previous report (Bharwad, Shah, Shrivastav, Sharma, & Singhal, 2020).…”

Section: Methodsmentioning

confidence: 75%

Selective quantification of lacosamide in human plasma using UPLC–MS/MS: Application to pharmacokinetic study in healthy subjects with different doses

Bharwad

Shah

Shrivastav

et al. 2020

Biomedical Chromatography

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Analysis of Nonsteroidal Anti-inflammatory Drugs by using Microfluidic Techniques: A Review

Fanali

D’Orazio

Gentili³

et al. 2021

CPA

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: In this review paper, miniaturized techniques, including both electromigration and liquid chromatographic ones, have been considered discussing their main features in the analytical field for the separation and analysis of nonsteroidal anti-inflammatory drugs (NSAIDs). In capillary electrophoresis (CE) and nano-liquid chromatography (nano-LC), separation are performed in capillaries with internal diameter (I.D.) lower than 100 m and therefore flow rates in the range 100- 1000 nL/min are applied. Therefore due to the low flow rate, high mass sensitivity can be obtained. Usually conventional UV detectors are used on-line; however these techniques can be coupled with mass spectrometry (MS). CE and nano-LC have been also applied to the separation of NSAIDs using silica stationary phases (SP) modified with C18 promoting interaction with analytes mainly based on hydrophobic interaction. In addition the use of chiral SP resulted effective for the chiral resolution of these compounds. In addition to silica phases, monolithic (both organic and inorganic) material has also been used. Although most of the presented studies aimed to demonstrate the usefulness of the considered microfluidic techniques, some applications to real samples have also been reported.

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Analysis of Different Methods of Extracting NSAIDs in Biological Fluid Samples for LC-MS/MS Assays: Scoping Review

et al. 2022

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The aim of this study was to carry out a systematic investigation and analysis of different drug extraction methods, specifically non-steroidal anti-inflammatory drugs in biological fluid samples, for Liquid Chromatography in Mass Spectrometry assays (LC-MS/MS). A search was carried out in the main databases between 1999 and 2021, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) checklist. Data were obtained through PubMed, Lilacs, Embase, Scopus, and Web of Science databases using the Boolean operators AND and OR. Studies were pre-selected by title and abstract by two independent reviewers. The selected texts were read in full, and only those that were complete and compatible with the inclusion and exclusion criteria were eligible for this research. A total of 248 references were obtained in the databases. After removing the duplicates and analyzing the titles and abstracts, 79 references were evaluated and passed to the next phase, which comprised the complete reading of the article. A total of 39 publications were eligible for this study. In 52% of the studies, the authors used the liquid–liquid extraction method (LLE), while in 41%, the solid-phase extraction method (SPE) was used. A total of 5% used microextraction methods and 2% used less-conventional techniques. The literature on the main methods used, the LLE and SPE methods, is extensive and consolidated; however, we found other studies that reported modifications of these traditional techniques, which were equally validated for use in LC-MS/MS. From this review, it is concluded that the diversity of techniques, reliability, and practical information about each analytical method used in this study can be adapted to advances in LC-MS/MS techniques; however, more ecological, economic, and sustainable approaches should be explored in the future.

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Quantification of fenoprofen in human plasma using UHPLC–tandem mass spectrometry for pharmacokinetic study in healthy subjects

Cited by 3 publications

References 15 publications

Selective quantification of lacosamide in human plasma using UPLC–MS/MS: Application to pharmacokinetic study in healthy subjects with different doses

Selective quantification of lacosamide in human plasma using UPLC–MS/MS: Application to pharmacokinetic study in healthy subjects with different doses

Analysis of Nonsteroidal Anti-inflammatory Drugs by using Microfluidic Techniques: A Review

Analysis of Different Methods of Extracting NSAIDs in Biological Fluid Samples for LC-MS/MS Assays: Scoping Review

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