Quantification of folate in fruits and vegetables: A fluorescence-based homogeneous assay

Martin, Harry; Comeskey, Daniel; Simpson, Robert M.; Laing, William A.; McGhie, Tony K.

doi:10.1016/j.ab.2010.03.032

Cited by 16 publications

(8 citation statements)

References 38 publications

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“…Currently, HPLC coupled with mass spectrometry (LC-MS-MS) is increasingly applied (Phillips et al, 2008;Vishnumohan et al, 2011). Alternative protein-binding and immunoassay methods exhibit unique advantages due to the inherent biospecific nature of the detection molecule (Alaburda et al, 2008;Martin et al, 2010). Besides, the novel photosynthetic proteins-based devices, called biosensors, have been successfully developed in food analysis applications with promising expectations for the detection of folate content (Lavecchia et al, 2011).…”

Section: Determination Of Folate Content In Ready-to-eat Productsmentioning

confidence: 99%

“…Chromatographic techniques involve two distinct steps after digestion of the samples: separation and purification of deconjugated extract, and detection and quantification of eluted monoglutamates (Arcot and Shrestha, 2005;Martin et al, 2010).…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…However, these technologies are limited by sensitivity or lack of selectivity and require complicated clean-up procedures as well as expensive affinity columns (AC) using folate-binding protein (FBP) for purification (Finglas et al, 1999;Martin et al, 2010). In order to overcome these barriers, the more recent use of HPLC, liquid chromatography-mass spectrometry (LC-MS) and/or liquid chromatography-tandem mass spectrometry (LC-MS/MS) presents the potential for measuring individual folate mono-glutamates with accuracy, precision, high sensitivity and selectivity at trace-level analyte, in complex samples, in a short time.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

See 2 more Smart Citations

Lack of data on folate in convenience foods: Should ready-to-eat products be considered relevant for folate intake? The European challenge

Fajardo

Alonso-Aperte

Varela‐Moreiras

2012

Journal of Food Composition and Analysis

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Section: Determination Of Folate Content In Ready-to-eat Productsmentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

See 1 more Smart Citation

Lack of data on folate in convenience foods: Should ready-to-eat products be considered relevant for folate intake? The European challenge

Fajardo

Alonso-Aperte

Varela‐Moreiras

2012

Journal of Food Composition and Analysis

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“…The published homogeneous binding assays for folate are based on fluorescence polarization and intensity 13 , chemiluminescence 14 , or enzyme labels with either bioluminescence 15 or absorbance measurement 16 . However, from the published homogeneous assay concepts only in CEDIA ® Red Blood Cell Folate (Roche Diagnostics/Boehringer Mannheim GmbH) assay whole blood is used as sample matrix.…”

Section: Introductionmentioning

confidence: 99%

Homogeneous Assay for Whole Blood Folate Using Photon Upconversion

et al. 2015

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Red blood cell folate is measured for folate deficiency diagnosis, because it reflects the long-term folate level in tissues, whereas serum folate only represents the dietary intake. Direct homogeneous assay from whole blood would be ideal but conventional fluorescence techniques in blood suffer from high background and strong absorption of light at ultraviolet and visible wavelengths. In this study, a new photon upconversion-based homogeneous assay for whole blood folate is introduced based on resonance energy transfer from upconverting nanophosphor donor coated with folate binding protein to a near-infrared fluorescent acceptor dye conjugated to folate analogue. The sensitized acceptor emission is measured at 740 nm upon 980 nm excitation. Thus, optically transparent wavelengths are utilized for both donor excitation and sensitized acceptor emission to minimize the sample absorption, and anti-Stokes detection completely eliminates the Stokes-shifted autofluorescence. The IC50 value of the assay was 6.0 nM and the limit of detection (LOD) was 1 nM. The measurable concentration range was 2 orders of magnitude between 1.0-100 nM, corresponding to 40-4000 nM folate in the whole blood sample. Recoveries of added folic acid were 112%-114%. A good correlation was found when compared to a competitive heterogeneous assay based on the DELFIA-technology. The introduced assay provides a simple and fast method for whole blood folate measurement.

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“…Currently, HPLC coupled with tandem mass spectrometry (LCeMSeMS) is increasingly applied to the analysis of folate in foods (Vishnumohan, Arcot, & Pickford, 2011). Alternative protein-binding and immunoassay methods exhibit unique advantages due to the inherent biospecific nature of the detection molecule (Ho, Hung, Wu, & Liao, 2009;Martin, Comeskey, Simpson, Laing, & McGhie, 2010;Prasad, Madhuri, Tiwari, & Sharma, 2010).…”

Section: Introductionmentioning

confidence: 99%

An optical biosensor assay for the determination of folate in milk and nutritional dairy products

Indyk

2011

International Dairy Journal

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Quantification of folate in fruits and vegetables: A fluorescence-based homogeneous assay

Cited by 16 publications

References 38 publications

Lack of data on folate in convenience foods: Should ready-to-eat products be considered relevant for folate intake? The European challenge

Lack of data on folate in convenience foods: Should ready-to-eat products be considered relevant for folate intake? The European challenge

Homogeneous Assay for Whole Blood Folate Using Photon Upconversion

An optical biosensor assay for the determination of folate in milk and nutritional dairy products

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