Quantification of Galantamine in &lt;i&gt;Sternbergia&lt;/i&gt; Species by High Performance Liquid Chromatography

Açıkara, Özlem Bahadır; Yılmaz, Bilal; Yazgan, Dilhun; Saltan, Gülçin

doi:10.4274/tjps.95967

Cited by 4 publications

(1 citation statement)

References 17 publications

(20 reference statements)

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“…In a study conducted by Tanker et al, S. clusiana was found to exhibit analgesic activity due to the presence of the major alkaloids, lycorine and hemanthamine [ 17 ]. Other studies have reported that S. clusiana species are important sources of the anticholinesterase agent galantamine, currently known for being highly effective in treating Alzheimer disease [ 18 , 19 ]. Besides its antifungal properties [ 20 ], S. clusiana was also suspected to have anticancerous potential due to its richness in Amaryllidaceae alkaloids (AA) such as lycorine [ 17 , 21 ], which was previously shown to be effective in the regulation of malignant cell invasion and metastasis [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro

Samarji

Younes

Khoury

et al. 2023

Plants

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Background: Sternbergia clusiana belongs to the Amaryllidaceae family and is recognized for the valuable biological activity of its major bioactive compounds. The aim of the current is to evaluate the anticancer effects of the ethanolic bulb extract of Sternbergia clusiana (ScBEE) on breast cancer cells in vitro and to further reveal the underlying cellular mechanism. Methods: An MTS cell viability assay was performed on MDA-MB-231 and MCF-7 cells, along with cell cycle analysis, cell death ELISA, Western blot analysis and an ROS production assay to decipher the mechanism of death. LC-MS/MS was also performed to identify the chemical composition of this ethanolic extract. Results: The results show a selective antiproliferative effect on both cell lines with no effect on normal mesenchymal stem cells. Further analysis suggested the activation of the apoptotic pathway as reflected by the increase in cellular and DNA fragmentation and alterations in apoptotic proteins such as Bax, Bcl-2 and c-PARP. ScBEE was also found to exhibit antioxidant effect, as shown by a decrease in ROS production. The underlying mechanism of action was explained by the presence of several bioactive compounds identified by LC-MS/MS, including alkaloids, terpenoids and phenols, which are elaborated in the manuscript. Conclusion: This study highlights the antioxidant and anticancerous properties of S.clusiana for breast cancer treatment.

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Section: Introductionmentioning

confidence: 99%

The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro

Samarji

Younes

Khoury

et al. 2023

Plants

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Rapid Determination of Galantamine in Human Plasma by Microchip Electrophoresis With a Highly Integrated Contactless Conductivity Detector

Li,

Lin,

Yang

2024

J of Separation Science

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A novel and rapid method was developed for the determination of galantamine in human plasma by microchip electrophoresis with a highly integrated contactless conductivity detector (CCD). The instrumental parameters affecting the response of the detector, such as excitation frequency and excitation voltage, were examined and optimized. The electrophoresis conditions that influenced the separation and detection of galantamine, including the composition of buffer solution, buffer pH, buffer concentration, additives, injection time, and separation voltage were systematically investigated. Under the optimal conditions, the peak height had a good linear relationship with the concentration of galantamine in human plasma from 10 to 160 µg/L, and the correlation coefficient was 0.9992, the limit of detection reached 1.1 µg/L. The recoveries were between 98.6% and 102.1%. This sensitive, rapid, and convenient method is a good alternative to existing methods for galantamine determination. Also, this highly integrated CCD holds great promise in clinical biochemical analysis.

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LC-MS bioanalysis of targeted nasal galantamine bound chitosan nanoparticles in rats’ brain homogenate and plasma

et al. 2021

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Quantification of Galantamine in <i>Sternbergia</i> Species by High Performance Liquid Chromatography

Cited by 4 publications

References 17 publications

The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro

The Antioxidant and Proapoptotic Effects of Sternbergia clusiana Bulb Ethanolic Extract on Triple-Negative and Estrogen-Dependent Breast Cancer Cells In Vitro

Rapid Determination of Galantamine in Human Plasma by Microchip Electrophoresis With a Highly Integrated Contactless Conductivity Detector

LC-MS bioanalysis of targeted nasal galantamine bound chitosan nanoparticles in rats’ brain homogenate and plasma

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