Quantification of growth and differentiation of C2C12 skeletal muscle cells on PSS–PAH-based polyelectrolyte layer-by-layer nanofilms

Ricotti, Leonardo; Taccola, Silvia; Bernardeschi, Irene; Dario, P.; Menciassi, Arianna

doi:10.1088/1748-6041/6/3/031001

Cited by 40 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was quantified by determination of fusion index and total myotube area (Fig. 4C,D) using established approaches (Ricotti et al, 2011). To examine possible effects of miR-1/miR-206 inhibition after the onset of differentiation, we modified miRNA levels, by antimiR-206 transfection at day 3 (in DM).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation

et al. 2012

View full text Add to dashboard Cite

SummaryMicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/ miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we identified novel target mRNAs by microarray and characterized their function in C2C12 myoblasts. Candidate targets from the screen were experimentally validated together with target genes that were predicted by three different algorithms. Some targets characterised have a known function in skeletal muscle development and/or differentiation and include Meox2, RARB, Fzd7, MAP4K3, CLCN3 and NFAT5, others are potentially novel regulators of myogenesis, such as the chromatin remodelling factors Smarcd2 and Smarcb1 or the anti-apoptotic protein SH3BGRL3. The expression profiles of confirmed target genes were examined during C2C12 cell myogenesis. We found that inhibition of endogenous miR-1 and miR-206 by antimiRs blocked the downregulation of most targets in differentiating cells, thus indicating that microRNA activity and target interaction is required for muscle differentiation. Finally, we show that sustained expression of validated miR-1 and/or miR-206 targets resulted in increased proliferation and inhibition of C2C12 cell myogenesis. In many cases the expression of genes related to non-muscle cell fates, such as chondrogenesis, was activated. This indicates that the concerted downregulation of multiple microRNA targets is not only crucial to the skeletal muscle differentiation program but also serves to prevent alternative cell fate choices.

show abstract

Section: Discussionmentioning

confidence: 99%

“…For adipogenic differentiation, C2C12 cells were grown to confluency in GM and switched to an adipogenic medium as described (Akimoto et al, 2005). Apoptosis was induced by 2 mM H 2 O 2 for 6 hours as described (Salucci et al, 2010).…”

Section: Dna Constructs and Luciferase Assaymentioning

confidence: 99%

Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation

et al. 2012

View full text Add to dashboard Cite

show abstract

“…However, up to date, few studies have combined this effect with the skeletal muscle behavior. In particular, different surface charges of a layer-by-layer polyelectrolyte on C2C12 skeletal muscle cells for skeletal 55 muscle regeneration capabilities was studied and it has been shown that negatively charged surfaces supported higher cell attachment than positively charged ones 49 .…”

mentioning

confidence: 99%

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

et al. 2013

View full text Add to dashboard Cite

This work reports on the influence of polarization and morphology of electroactive poly(vinylidene fluoride), PVDF, on the biological response of myoblast cells. Non-poled, ''poled +'' and "poled-" -PVDF were prepared in the form of films. Further, random and aligned electrospun -PVDF fiber mats were also prepared. It is demonstrated that negatively charged surfaces improve cell adhesion and proliferation and that the directional growth of the myoblast cells can be achieved by the cell culture on oriented fibers. Therefore, 10 the potential application of electroative materials for muscle regeneration is demonstrated.

show abstract

“…Confluent cultured of C2C12cells washed with PBS, detached from petri dish by trypsinizing (.25% trypsin, Sigma Co., St. Louis, MO). Trypsinated cell-medium solution was centrifuged to get cell pallet to seed cell on the PV devices @ 12,000/cm 2 [17]. The cell cultures were maintained in DMEM growth medium and incubated maintaining 5% CO 2 atmosphere at 37°C, and 100% humidity.…”

Section: Methodsmentioning

confidence: 99%

Silicon substrate as a novel cell culture device for myoblast cells

et al. 2014

View full text Add to dashboard Cite

BackgroundTissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy.ResultsIt was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate.ConclusionsThis study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.

show abstract

Quantification of growth and differentiation of C2C12 skeletal muscle cells on PSS–PAH-based polyelectrolyte layer-by-layer nanofilms

Cited by 40 publications

References 29 publications

Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation

Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

Silicon substrate as a novel cell culture device for myoblast cells

Contact Info

Product

Resources

About