Quantification of growth factors in advanced platelet‐rich fibrin and concentrated growth factors and their clinical efficacy as adjunctive to the GTR procedure in periodontal intrabony defects

Lei, Lihong; Yu, Yuanyuan; Han, Jiayin; Danhui, Shi; Sun, Weilian; Zhang, Diya; Chen, Lili

doi:10.1002/jper.19-0290

Cited by 70 publications

(81 citation statements)

References 39 publications

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“…This hyper-concentrated growth factors media may result in a cytotoxic environment under in vitro conditions. This hypothesis is in agreement with previous reports that highlight the role of the fibrin network in the release kinetics of growth factors [3,45,46].…”

Section: Discussionsupporting

confidence: 94%

Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour

Fernandez-Medina

Vaquette

Ivanovski

2019

IJMS

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Hemoderivatives have utilized in an empirical manner, driven by clinical considerations, leading to the development of a plethora of manufacturing protocols. The purpose of this study was to investigate the composition and bioactivity of four common clinical-grade hemoderivates prepared using standardised methods. Four different hemoderivatives were obtained from sheep blood and divided into two groups: A-PRF/i-PRF (fresh) and P-PRP/L-PRP (anticoagulated). Thrombus (CLOT) was used as a control. Thrombocyte quantification, growth factor composition (IGF-I, VEGF, PDGF-BB, BMP-2), cell viability, migration and mineralization assay were evaluated. Platelet recovery was superior for L-PRP followed by P-PRP. A significant cumulative release of IGF-I and PDGF-BB was noted for A-PRF and L-PRP groups at early time points. Similar release profiles of BMP-2 and VEGF were noted in all protocols. Cell viability and migration assay have demonstrated a detrimental effect when the concentration was ≥60%. Moreover, at Day 21, i-PRF have demonstrated superior mineralisation properties when compared to all groups. A negative impact of A-PRF was demonstrated at high concentrations. Despite its low content in growth factors, i-PRF was the best performing blood product for inducing osteoblast mineralisation, and therefore could be the candidate of choice for utilisation in bone tissue engineering applications.

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Section: Discussionsupporting

confidence: 94%

Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour

Fernandez-Medina

Vaquette

Ivanovski

2019

IJMS

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“…We believe, therefore, that the growth factors in CGF could stimulate the proliferation of mesenchymal cells, but they do not have the ability to differentiate undifferentiated mesenchymal cells into osteoblasts or chondroblasts. The histological findings were consistent with the report that the concentration of BMP-2 in CGF was fairly low and almost undetectable by ELISA [ 29 ]. The addition of 1.0 µg of rhBMP-2 to the CGF membrane (5 × 5 × 2 mm 3 ) might provide synergistic effects with several growth factors in CGF for bone and cartilage induction.…”

Section: Discussionsupporting

confidence: 91%

Human Fresh Fibrin Membrane with Bone Morphogenetic Protein-2 (BMP-2) Induces Bone Formation in the Subcutaneous Tissues of Nude Mice

et al. 2020

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Autologous blood-derived fibrin glue with platelets, called the concentrated growth factor (CGF), can be prepared immediately by only the decided centrifuge without the addition of coagulation factors. Collagen materials combined with recombinant human BMP-2 have been commercially available for clinical use. The fresh CGF is auto-clot with wettability and elasticity, while most collagen membranes are derived from the cow or pig. The fresh CGF has wettability and elasticity, while collagen membranes are dry materials without elasticity. The aim of this study was to observe the microstructures of human CGF membrane and evaluate its behavior as a delivery scaffold of rhBMP-2 in the subcutaneous tissues of nude mice. Twenty-four nude mice (5-week-old, male) were used for the assessment of in vivo ectopic bone formation. Mice were received the CGF membrane as the controls and the CGF/rhBMP-2 membrane as the experimental group in the subcutaneous tissues, and harvested at 7, 10, and 14 days after the graft. Harvested samples were evaluated for the histological examination and the histomorphometric measurement was conducted to compare the residue of the CGF, as well as the new bone. Mature fibrin fibers assembled from multiple fibrillary elements and platelets with the rhBMP-2 membrane induced several bony islands and cartilage without residues of CGF at 14 days, while the CGF membrane alone was almost absorbed at 10 days and failed to induce bone formation at 14 days. These results demonstrated that the fresh, human CGF membrane could contribute to a short-term, sticky fibrin matrix for the delivery of rhBMP-2.

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“…In this regard, with excluding case series (without a control group), 18 studies on bone regeneration and six studies on regeneration of other tissues are listed in Tables and . Two articles were randomized clinical trials and one was a cohort study in which the treated pathologies were peri‐implantitis, guided tissue regeneration (GTR), and gingival recessions (Bozkurt Dogan et al, ; Isler et al, ; Lei et al, ). Most studies stated improvement of tissue healing or regeneration in the presence of CGF, yet three studies investigating its effect on peri‐implantitis, implant stability, and immediate implants reported no significant difference or lower effect of CGF (Isler et al, ; Özveri Koyuncu, İçpınar Çelik, et al, ; Yang et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…Combination of CGF with stem cells or grafts resulted in better results than CGF alone (Chen et al, ; Durmuslar et al, ; Honda et al, ; Kizilaslan et al, ; Qiao et al, ; Wang et al, ). Three studies compared CGF to other platelets concentrates; one study reported no significant difference between CGF, PRP, and PRF on bone formation in rabbit‐skull defect (Kim et al, ), another study also confirmed no significant difference in GTR between A‐PRF and CGF (Lei et al, ); while Park et al indicated better bone formation of CGF than PRF in femur defect of adult dogs (Park et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…In this regard, with excluding case series (without a control group), 18 studies on bone regeneration and six studies on regeneration of other tissues are listed inTables 4 and 5. Two articles were randomized clinical trials and one was a cohort study in which the treated pathologies were peri-implantitis, guided tissue regeneration (GTR), and gingival recessions(Bozkurt Dogan et al, 2015;Isler et al, 2018;Lei et al, 2019).…”

mentioning

confidence: 99%

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In vitro and in vivo effects of concentrated growth factor on cells and tissues

Tabatabaei

Aghamohammadi

Tayebi

2020

J Biomedical Materials Res

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This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty‐five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, freeze‐dried, membrane, extract, or exudate. Most studies demonstrate the positive effects of CGF in a dose‐dependent manner under certain concentrations. Studies comparing CGF with other platelet concentrates, report lower efficiency, no statistically significant differences, or better results for CGF. Combination of CGF with stem cells and biomaterials significantly improves bone regeneration and the effect of allograft or collagen membrane is better than CGF alone. For a better examination of the biological outcomes of CGF, the standardization of CGF preparation regarding the choice of the test tube material for blood collection, the required volume of blood, the necessary count of platelets in CGF, and the most appropriate type of CGF are recommended.

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Quantification of growth factors in advanced platelet‐rich fibrin and concentrated growth factors and their clinical efficacy as adjunctive to the GTR procedure in periodontal intrabony defects

Cited by 70 publications

References 39 publications

Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour

Systematic Comparison of the Effect of Four Clinical-Grade Platelet Rich Hemoderivatives on Osteoblast Behaviour

Human Fresh Fibrin Membrane with Bone Morphogenetic Protein-2 (BMP-2) Induces Bone Formation in the Subcutaneous Tissues of Nude Mice

In vitro and in vivo effects of concentrated growth factor on cells and tissues

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